Anti-Estrogen Receptor alpha (phospho S167) antibody (ab31478)
Key features and details
- Rabbit polyclonal to Estrogen Receptor alpha (phospho S167)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Estrogen Receptor alpha (phospho S167) antibody
See all Estrogen Receptor alpha primary antibodies -
Description
Rabbit polyclonal to Estrogen Receptor alpha (phospho S167) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Estrogen Receptor alpha (phospho S167).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab31478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 68 kDa.
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IHC-P | (1) |
1/50 - 1/100.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 68 kDa. |
IHC-P
1/50 - 1/100. |
Target
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Function
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
Post-translational
modificationsPhosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
Ubiquitinated. Deubiquitinated by OTUB1.
Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
Palmitoylated (isoform 3). Not biotinylated (isoform 3). -
Cellular localization
Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation. - Information by UniProt
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Database links
- Entrez Gene: 2099 Human
- Omim: 133430 Human
- SwissProt: P03372 Human
- Unigene: 208124 Human
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Alternative names
- 7*/654 isoform antibody
- 7*/819 2 isoform antibody
- 7*/822 isoform antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded breast carcinoma, using ab31478 at 1/50. Left: Untreated; Right: Treated with synthesized phosphopeptide.
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All lanes : Anti-Estrogen Receptor alpha (phospho S167) antibody (ab31478) at 1/500 dilution
Lane 1 : HeLa cells treated with EGF
Lane 2 : Untreated HeLa cell lysate
Predicted band size: 68 kDa -
ab31478 staining human non-small cell lung cancer tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in 10 mM sodium citrate (pH 6) prior to blocking with a commercially available blocking reagent for 20 minutes at 22°C. The primary antibody was diluted 1/200 and incubated with the sample for 24 hours at 4°C. A HRP conjugated antibody was used as the secondary.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab31478 has been referenced in 6 publications.
- Iida M et al. Compensatory role of insulin-like growth factor 1 receptor in estrogen receptor signaling pathway and possible therapeutic target for hormone therapy-resistant breast cancer. Breast Cancer 26:272-281 (2019). PubMed: 30328006
- Zhao G et al. MicroRNA-34a suppresses cell proliferation by targeting LMTK3 in human breast cancer mcf-7 cell line. DNA Cell Biol 32:699-707 (2013). WB . PubMed: 24050776
- Lucchetti C et al. The prolyl isomerase Pin1 acts synergistically with CDK2 to regulate the basal activity of estrogen receptor a in breast cancer. PLoS One 8:e55355 (2013). PubMed: 23390529
- Bobustuc GC et al. MGMT inhibition restores ERa functional sensitivity to antiestrogen therapy. Mol Med 18:913-29 (2012). PubMed: 22549111
- Skliris GP et al. A phosphorylation code for oestrogen receptor-alpha predicts clinical outcome to endocrine therapy in breast cancer. Endocr Relat Cancer 17:589-97 (2010). PubMed: 20418363
- Márquez-Garbán DC et al. Estrogen receptor signaling pathways in human non-small cell lung cancer. Steroids 72:135-43 (2007). PubMed: 17276470