Overview

  • Product name

    Anti-Estrogen Receptor alpha (phospho S294) antibody [BUI-1-37]
    See all Estrogen Receptor alpha primary antibodies
  • Description

    Rabbit monoclonal [BUI-1-37] to Estrogen Receptor alpha (phospho S294)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Estrogen Receptor alpha aa 250-350 (phospho S294).
    Database link: P03372

  • General notes

    Western blot protocol advice:

    Since intracellular levels of pS294 ER are very low, Western blot without prior immunoprecipitation of total ER is not sensitive enough to detect pS294 ER. We recommend initial ER immunoprecipitation, following which it is possible to use this antibody for Western blot (see data image).

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab207602 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 66 kDa.
IP Use at an assay dependent concentration.

Target

  • Function

    Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Post-translational
    modifications

    Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
    Glycosylated; contains N-acetylglucosamine, probably O-linked.
    Ubiquitinated. Deubiquitinated by OTUB1.
    Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
    Palmitoylated (isoform 3). Not biotinylated (isoform 3).
  • Cellular localization

    Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
  • Information by UniProt
  • Database links

  • Alternative names

    • 7*/654 isoform antibody
    • 7*/819 2 isoform antibody
    • 7*/822 isoform antibody
    • 8*/901 isoform antibody
    • 8*/941 isoform antibody
    • DKFZp686N23123 antibody
    • ER alpha antibody
    • ER antibody
    • ER-alpha antibody
    • Era antibody
    • ESR antibody
    • ESR1 antibody
    • ESR1_HUMAN antibody
    • ESRA antibody
    • Estradiol receptor antibody
    • Estrogen nuclear receptor alpha antibody
    • Estrogen receptor 1 antibody
    • Estrogen receptor alpha 3*,4,5,6,7*/822 isoform antibody
    • Estrogen receptor alpha antibody
    • Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform antibody
    • Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform antibody
    • Estrogen receptor alpha delta 4 +49 isoform antibody
    • Estrogen receptor alpha delta 4*,5,6,7*/654 isoform antibody
    • Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform antibody
    • Estrogen receptor alpha E1 E2 1 2 antibody
    • Estrogen receptor alpha E1 N2 E2 1 2 antibody
    • Estrogen receptor antibody
    • ESTRR antibody
    • NR3A1 antibody
    • Nuclear receptor subfamily 3 group A member 1 antibody
    see all

Images

  • MCF7 cells growing in phenol red-free medium supplemented with 5% charcoal stripped serum for 24 hours were treated for 25 minutes with vehicle (C = control), 10nM E2, or pretreated for 20 minutes with 1uM Dinaciclib (CDK2 inhibitor) and then 25 minutes with E2 treatment (Din/E2). Harvested cells were lysed and immunoprecipitated using an ERα N-terminal directed rabbit polyclonal antibody; and the immunoprecipitated ERα (45 ml final volume each from C, E2 and Din/E2 samples) was used to prepare separate western blots probed with either ab207602 anti-ERpS294 monoclonal, or antibodies specific for ERpS118 or total ERα, all of which run at 67 kDa molecular weight.

    Alternatively, cell lysates can first be immunoprecipitated with ab207602 anti-ERpS294 monoclonal and the immunoprecipitate then western blotted with other antibodies specific for ERα (not shown).

References

This product has been referenced in:

  • Helzer KT  et al. The Phosphorylated Estrogen Receptor Cistrome Identifies a Subset of Active Enhancers Enriched for Direct ER-DNA Binding and the Transcription Factor GRHL2. Mol Cell Biol N/A:N/A (2018). Read more (PubMed: 30455249) »
  • Scott GK  et al. ERpS294 is a biomarker of ligand or mutational ERa activation and a breast cancer target for CDK2 inhibition. Oncotarget N/A:N/A (2016). Human . Read more (PubMed: 27765908) »
See all 2 Publications for this product

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