Product nameAnti-Estrogen Receptor alpha (phospho S294) antibody [BUI-1-37]
See all Estrogen Receptor alpha primary antibodies
DescriptionRabbit monoclonal [BUI-1-37] to Estrogen Receptor alpha (phospho S294)
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human Estrogen Receptor alpha aa 250-350 (phospho S294).
Database link: P03372
Western blot protocol advice:
Since intracellular levels of pS294 ER are very low, Western blot without prior immunoprecipitation of total ER is not sensitive enough to detect pS294 ER. We recommend initial ER immunoprecipitation, following which it is possible to use this antibody for Western blot (see data image).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab207602 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 66 kDa.|
|IP||Use at an assay dependent concentration.|
FunctionNuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
modificationsPhosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
Ubiquitinated. Deubiquitinated by OTUB1.
Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
Palmitoylated (isoform 3). Not biotinylated (isoform 3).
Cellular localizationNucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
- Information by UniProt
- 7*/654 isoform antibody
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MCF7 cells growing in phenol red-free medium supplemented with 5% charcoal stripped serum for 24 hours were treated for 25 minutes with vehicle (C = control), 10nM E2, or pretreated for 20 minutes with 1uM Dinaciclib (CDK2 inhibitor) and then 25 minutes with E2 treatment (Din/E2). Harvested cells were lysed and immunoprecipitated using an ERα N-terminal directed rabbit polyclonal antibody; and the immunoprecipitated ERα (45 ml final volume each from C, E2 and Din/E2 samples) was used to prepare separate western blots probed with either ab207602 anti-ERpS294 monoclonal, or antibodies specific for ERpS118 or total ERα, all of which run at 67 kDa molecular weight.
Alternatively, cell lysates can first be immunoprecipitated with ab207602 anti-ERpS294 monoclonal and the immunoprecipitate then western blotted with other antibodies specific for ERα (not shown).
This product has been referenced in:
- Helzer KT et al. The Phosphorylated Estrogen Receptor Cistrome Identifies a Subset of Active Enhancers Enriched for Direct ER-DNA Binding and the Transcription Factor GRHL2. Mol Cell Biol N/A:N/A (2018). Read more (PubMed: 30455249) »
- Scott GK et al. ERpS294 is a biomarker of ligand or mutational ERa activation and a breast cancer target for CDK2 inhibition. Oncotarget N/A:N/A (2016). Human . Read more (PubMed: 27765908) »