Key features and details
- Mouse monoclonal [ESR1/1935] to Estrogen Receptor
- Suitable for: Protein Array, IHC-P, WB
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-Estrogen Receptor antibody [ESR1/1935]
See all Estrogen Receptor primary antibodies
DescriptionMouse monoclonal [ESR1/1935] to Estrogen Receptor
Tested applicationsSuitable for: Protein Array, IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human Estrogen Receptor aa 1-595.
Database link: P03372
- IHC-P: Human endometrial carcinoma and human breast carcinoma tissue. WB: MCF7 cell lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from Bioreactor concentrate.
Light chain typekappa
Our Abpromise guarantee covers the use of ab237995 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Protein Array||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 1 - 2 µg/ml.
Primary incubation for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 66 kDa.|
FunctionNuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
modificationsPhosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
Ubiquitinated. Deubiquitinated by OTUB1.
Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
Palmitoylated (isoform 3). Not biotinylated (isoform 3).
Cellular localizationNucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
- Information by UniProt
- Atherosclerosis, susceptibility to, included antibody
- DKFZp686N23123 antibody
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Anti-Estrogen Receptor antibody [ESR1/1935] (ab237995) at 1 µg/ml + MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Predicted band size: 66 kDa
Formalin-fixed, paraffin-embedded human Breast Carcinoma tissue stained for Estrogen Receptor using ab237995 at 2 µg/mL in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human Endometrial Carcinoma tissue stained for Estrogen Receptor using ab237995 at 2 µg/mL in immunohistochemical analysis.
ab237995 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237995 has not yet been referenced specifically in any publications.