Overview

  • Product name

    Anti-Estrogen Receptor antibody [ESR1/1935] - BSA and Azide free
    See all Estrogen Receptor primary antibodies
  • Description

    Mouse monoclonal [ESR1/1935] to Estrogen Receptor - BSA and Azide free
  • Host species

    Mouse
  • Specificity

    This antibody is specific to estrogen receptor alpha (ER alpha) and shows minimal cross-reaction with other members of the family.

  • Tested applications

    Suitable for: Protein Array, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human Estrogen Receptor.
    Database link: P03372

  • Positive control

    • IHC-P: Human endometrial carcinoma and breast carcinoma tissue. WB: MCF7 cell lysate.
  • General notes

    ab237837 is a PBS-only buffer format of ab237995.

Properties

Applications

Our Abpromise guarantee covers the use of ab237837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Protein Array Use at an assay dependent concentration.
IHC-P Use a concentration of 1 - 2 µg/ml.

Primary incubation for 30 minutes at room temperature.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.

WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 66 kDa.

Target

  • Function

    Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Post-translational
    modifications

    Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
    Glycosylated; contains N-acetylglucosamine, probably O-linked.
    Ubiquitinated. Deubiquitinated by OTUB1.
    Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
    Palmitoylated (isoform 3). Not biotinylated (isoform 3).
  • Cellular localization

    Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
  • Information by UniProt
  • Database links

  • Alternative names

    • Atherosclerosis, susceptibility to, included antibody
    • DKFZp686N23123 antibody
    • ER Alpha antibody
    • ER antibody
    • ER Beta antibody
    • ER-alpha antibody
    • ER[a] antibody
    • ER[b] antibody
    • Era antibody
    • ERalpha antibody
    • Erb antibody
    • Erb2 antibody
    • ERbeta antibody
    • ESR antibody
    • ESR BETA antibody
    • ESR1 antibody
    • ESR1_HUMAN antibody
    • ESR2 antibody
    • ESRA antibody
    • ESRB antibody
    • Estr antibody
    • Estra antibody
    • Estradiol Receptor alpha antibody
    • Estradiol receptor antibody
    • Estradiol Receptor beta antibody
    • ESTRB antibody
    • Estrogen receptor 1 (alpha) antibody
    • Estrogen Receptor 1 antibody
    • Estrogen receptor 2 (ER beta) antibody
    • Estrogen Receptor 2 antibody
    • Estrogen receptor 2 ER beta antibody
    • Estrogen receptor alpha antibody
    • Estrogen receptor antibody
    • Estrogen receptor beta 4 antibody
    • Estrogen resistance, included antibody
    • HDL cholesterol, augmented response of, to hormone replacement, included antibody
    • Myocardial infarction, susceptibility to, included antibody
    • NR3A1 antibody
    • NR3A2 antibody
    • Nuclear receptor subfamily 3 group A member 1 antibody
    • Nuclear receptor subfamily 3 group A member 2 antibody
    • OTTHUMP00000017718 antibody
    • OTTHUMP00000017719 antibody
    • RNESTROR antibody
    see all

Images

  • Anti-Estrogen Receptor antibody [ESR1/1935] (ab237995) at 1 µg/ml + MCF7 (human breast adenocarcinoma cell line) cell lysate

    Predicted band size: 66 kDa



    This data was produced with ab237995, the same antibody in a different formulation with BSA and Azide.

  • Formalin-fixed, paraffin-embedded human endometrial carcinoma tissue stained for Estrogen Receptor using ab237995 at 2 μg/ml in immunohistochemical analysis.

    This data was produced with ab237995, the same antibody in a different formulation with BSA and Azide.

  • Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for Estrogen Receptor using ab237995 at 2 μg/ml in immunohistochemical analysis.

    This data was produced with ab237995, the same antibody in a different formulation with BSA and Azide.

  • This data was produced with ab237995, the same antibody in a different formulation with BSA and Azide.
    ab237995 was tested in protein array against over 19000 different full-length human proteins.
    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
    A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

References

ab237837 has not yet been referenced specifically in any publications.

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