Anti-Estrogen Receptor beta antibody [14C8] (ab288)
Key features and details
- Mouse monoclonal [14C8] to Estrogen Receptor beta
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-Estrogen Receptor beta antibody [14C8]
See all Estrogen Receptor beta primary antibodies -
Description
Mouse monoclonal [14C8] to Estrogen Receptor beta -
Host species
Mouse -
Tested Applications & Species
Application Species IHC-P HumanWB Human -
Immunogen
Recombinant fragment corresponding to Human Estrogen Receptor beta aa 1-153. Expressed in E.coli.
Database link: Q92731 -
Positive control
- WB: Recombinant fusion protein, human liver and intestine, HEK-293T cells. IHC-P: Endocervical epithelium, Lobular breast carcinoma and human breast carcinoma.
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General notes
This product was changed from ascites to tissue culture supernatant on 23rd October 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
14C8 -
Myeloma
NS1 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab288 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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IHC-P |
Human
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WB |
Human
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Application | Abreviews | Notes |
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WB | (2) |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 59 kDa.
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IHC-P | (5) |
Use a concentration of 5 µg/ml.
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Notes |
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WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 59 kDa. |
IHC-P
Use a concentration of 5 µg/ml. |
Target
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Function
Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual. -
Tissue specificity
Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2100 Human
- Omim: 601663 Human
- SwissProt: Q92731 Human
- Unigene: 660607 Human
- Unigene: 729020 Human
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Alternative names
- ER BETA antibody
- ER-beta antibody
- Erb antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta antibody [14C8] (ab288)
Paraffin embedded human breast carcinoma tissue stained for Estrogen Receptor beta using ab288 at 1/200 dilution in immunohistochemical analysis.
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All lanes : Anti-Estrogen Receptor beta antibody [14C8] (ab288) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T whole cell extracts
Lane 2 : Transfected HEK-293T whole cell extracts
Lysates/proteins at 30 µg per lane.
Predicted band size: 59 kDa7.5% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta antibody [14C8] (ab288)
ab288 staining Estrogen Receptor beta in infiltrating lobular carcinoma of the human breast tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde. Endogenous peroxidase blocking was with 3% H2O2 at room temperature for 30 minutes. Blocking with 1.5% goat serum (dilute goat serum by 1xPBS), at room temperature for 30min; antigen retrieval was by heat mediation in a citrate buffer, pH 6.0, 15min (Cuisinart Electric Pressure Cooker #EPC-1200, choose "high pressure"). Samples were incubated with primary antibody (1/100) for overnight at 4°C. An ABC HRP Kit Mouse IgG) was used as the secondary antibody (1/200), room temperature for 30min. Washed twice for 5 minutes using PBS. DAB was used as a substrate.
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All lanes : Anti-Estrogen Receptor beta antibody [14C8] (ab288) at 1/500 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Human liver tissue lysate
Lane 3 : Human intestine tissue lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Mouse IgG antibody (HRP) at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 59 kDa7.5% gel.
Running condition: 80V, 15min; 140V, 40min.
Transfer condition: Semi-dry, 18 V, 60min (NC membrane)
blocking condition: 5% non-fat milk in TBST, RT, 60min.
primary antibody incubation: 4°C overnight.
Secondary antibody incubation: room temperature for 1 hour.
Washing conditions: 5 ml TBST, 4 x 5min.
ECL exposure.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta antibody [14C8] (ab288)ab288 staining endocervical epithelium by IHC-P.
Protocols
References (79)
ab288 has been referenced in 79 publications.
- Gu J et al. Targeting the ERß/Angiopoietin-2/Tie-2 signaling-mediated angiogenesis with the FDA-approved anti-estrogen Faslodex to increase the Sunitinib sensitivity in RCC. Cell Death Dis 11:367 (2020). PubMed: 32409702
- Li H et al. CLPTM1L induces estrogen receptor ß signaling-mediated radioresistance in non-small cell lung cancer cells. Cell Commun Signal 18:152 (2020). PubMed: 32943060
- Song P et al. Estrogen receptor ß inhibits breast cancer cells migration and invasion through CLDN6-mediated autophagy. J Exp Clin Cancer Res 38:354 (2019). PubMed: 31412908
- Datta N et al. Promyelocytic Leukemia (PML) gene regulation: implication towards curbing oncogenesis. Cell Death Dis 10:656 (2019). PubMed: 31506431
- López-Romero R et al. The expression of transcription factor BORIS and its association with the estrogen receptor beta (ER-ß) in cervical carcinogenesis. Int J Clin Exp Pathol 12:3208-3221 (2019). PubMed: 31934165