Anti-Estrogen Receptor beta antibody [14C8] (ab288)
- Datasheet
- Specific References (59)
- Protocols
Overview
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Product nameAnti-Estrogen Receptor beta antibody [14C8]
See all Estrogen Receptor beta primary antibodies -
DescriptionMouse monoclonal [14C8] to Estrogen Receptor beta
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Host speciesMouse
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Specificity14C8 is specific for human ER-beta. The antibody has been tested in WB against recombinant protein. It has not yet been tested in our laboratory using any cell lysates and several publications suggest it can give weak bands in breast tumour extracts lysate. Strong Erb staining in epithelial cell nuclei. Occasional weak to moderate staining is seen in surrounding stromal and endothelial cell nuclei. Sporadic light cytoplasmic staining is sometimes observed. We have also successfully detected ERb in colon and ovarian tumors using the same antibody.
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Tested applicationsSuitable for: WB, Flow Cyt, IHC-P, ICC/IF, ChIP, IHC-Frmore details
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Species reactivityReacts with: Mouse, Human, Monkey, Baboon
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Immunogen
Recombinant 6-His fusion protein encoding amino acids 1-153 of human ER-beta expressed in E. coli.
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Positive control
- WB: Recombinant fusion protein. IHC-P: endocervical epithelium, Lobular breast carcinoma.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.40
Constituent: 0.0268% PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityMonoclonal
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Clone number14C8
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MyelomaNS1
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IsotypeIgG2b
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Light chain typekappa
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Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Conjugation kits
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Immunohistochemistry kits
Applications
Our Abpromise guarantee covers the use of ab288 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 59 kDa. | |
| Flow Cyt | 1/25. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
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| IHC-P | Use a concentration of 5 µg/ml. | |
| ICC/IF | Use at an assay dependent concentration. | |
| ChIP | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. |
Target
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FunctionNuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.
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Tissue specificityIsoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.
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Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
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Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 2100 Human
- Entrez Gene: 13983 Mouse
- Omim: 601663 Human
- SwissProt: Q92731 Human
- SwissProt: O08537 Mouse
- Unigene: 660607 Human
- Unigene: 729020 Human
- Unigene: 2561 Mouse
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Alternative names
- ER BETA antibody
- ER-beta antibody
- Erb antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta antibody [14C8] (ab288)](https://www.abcam.com/ps/products/0/ab288/Images/ab288_5.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta antibody [14C8] (ab288)ab288 staining endocervical epithelium by IHC-P. -
![Western blot - Anti-Estrogen Receptor beta antibody [14C8] (ab288)](https://www.abcam.com/ps/products/0/ab288/Images/ab288-274249-ab288-ERb-WB-supplier.PNG)
Western blot - Anti-Estrogen Receptor beta antibody [14C8] (ab288)All lanes : Anti-Estrogen Receptor beta antibody [14C8] (ab288) at 1/5000 dilution
Lane 1 : Non-transfected 293T whole cell extracts
Lane 2 : Transfected 293T whole cell extracts
Lysates/proteins at 30 µg per lane.
Performed under non-reducing conditions.
Predicted band size: 59 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta antibody [14C8] (ab288)](https://www.abcam.com/ps/products/0/ab288/Images/ab288_6.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta antibody [14C8] (ab288)ab288 staining infiltrating lobular carcinoma of the breast by IHC-P.
Protocols
References
This product has been referenced in:
- Tang YQ et al. Fructus Ligustri Lucidi modulates estrogen receptor expression with no uterotrophic effect in ovariectomized rats. BMC Complement Altern Med 18:118 (2018). Read more (PubMed: 29609586) »
- Alexander BH et al. Stable Density and Dynamics of Dendritic Spines of Cortical Neurons Across the Estrous Cycle While Expressing Differential Levels of Sensory-Evoked Plasticity. Front Mol Neurosci 11:83 (2018). Read more (PubMed: 29615867) »
Customer reviews and Q&As
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