Anti-Estrogen Receptor beta antibody (ab3576)
- Datasheet
- Specific References (32)
- Protocols
Overview
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Product nameAnti-Estrogen Receptor beta antibody
See all Estrogen Receptor beta primary antibodies -
DescriptionRabbit polyclonal to Estrogen Receptor beta
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Host speciesRabbit
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SpecificityBy Western blot, this antibody detects an ~55 kDa protein representing ER beta from Rat brain homogenate. Immunocytochemical staining of ER beta in cells overexpressing the Human protein results in nuclear staining.
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Tested applicationsSuitable for: IHC-P, IHC-Fr, ICC, WB, Inhibition Assay, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rat, Sheep, Human, Pig, Non human primates
Predicted to work with: Hamster
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Immunogen
Synthetic peptide corresponding to Rat Estrogen Receptor beta aa 467-485.
Sequence:CSSTEDSKNKESSQNLQSQ
(Peptide available asab5018) -
Positive control
- WB: Rat brain lysate IHC: testis, efferent ductules, epididymis, and vas deferens (see Zhou et al), Rat ovary (see Sanchez-Criado)
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
Applications
Our Abpromise guarantee covers the use of ab3576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. | |
| IHC-Fr | 1/50. | |
| ICC | 1/100 - 1/1000. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 59 kDa).Can be blocked with Estrogen Receptor beta peptide (ab5018). | |
| Inhibition Assay | Use at an assay dependent concentration. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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FunctionNuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.
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Tissue specificityIsoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.
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Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
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Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 2100 Human
- Entrez Gene: 13983 Mouse
- Entrez Gene: 396697 Pig
- Entrez Gene: 25149 Rat
- Omim: 601663 Human
- SwissProt: Q92731 Human
- SwissProt: O08537 Mouse
- SwissProt: Q9XSW2 Pig
see all -
Alternative names
- ER BETA antibody
- ER-beta antibody
- Erb antibody
see all
Images
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Immunohistochemistry (Frozen sections) - Anti-Estrogen Receptor beta antibody (ab3576)Image from Novensà L et al., PLoS One. 2011;6(9):e25335. Epub 2011 Sep 22. Fig 4.; doi:10.1371/journal.pone.0025335; PLoS ONE 6(9): e25335.Immunofluorescence analysis of frozen murine thoracic aorta, staining Estrogen Receptor beta with ab3576 at 1/50 dilution (green: not merged with nucleus, or purple: merged with nucleus). Nuclei were stained with DAPI (blue) and actin fibers were stained red. -
Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor beta antibody (ab3576)Immunofluorescent analysis using Estrogen Receptor beta polyclonal antibody (ab3576) shows nuclear localization of Estrogen Receptor beta in MCF7 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% BSA for 15 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing Estrogen Receptor beta (ab3576), at a dilution of 1:100 for 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification. -
Western blot - Anti-Estrogen Receptor beta antibody (ab3576)All lanes : Anti-Estrogen Receptor beta antibody (ab3576) at 1/2000 dilution
Lane 1 : CAL region of Human brain - Whole cell lysate
Lane 2 : EC region of Human brain - Whole cell lysate
Lane 3 : HIP region of Human brain - Whole cell lysate
Lane 4 : IT region of Human brain - Whole cell lysate
Lane 5 : MF region of Human brain - Whole cell lysate
Lane 6 : SP region of Human brain - Whole cell lysate
Secondary
All lanes : HRP conjugated Donkey Anti-rabbit IgG
Predicted band size: 59 kDa
Observed band size: 55 kDa (why is the actual band size different from the predicted?)
This image is courtesy of an Abreview submitted on 3 February 2006. -
Immunocytochemistry - Anti-Estrogen Receptor beta antibody (ab3576)Immunolocalization of ER beta from CHO cells overexpressing the Human protein, using ab3576 (1ug/ml).
Protocols
References
This product has been referenced in:
- Yan L et al. Effects of Ovariectomy in an hSOD1-G93A Transgenic Mouse Model of Amyotrophic Lateral Sclerosis (ALS). Med Sci Monit 24:678-686 (2018). Read more (PubMed: 29394243) »
- Seo SY et al. An estradiol-independent BDNF-NPY cascade is involved in the antidepressant effect of mechanical acupuncture instruments in ovariectomized rats. Sci Rep 8:5849 (2018). Read more (PubMed: 29643431) »
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