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    estrogen-receptor-beta-antibody-ab3576.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
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Anti-Estrogen Receptor beta antibody (ab3576)

  • Datasheet
  • SDS
Reviews (8)Q&A (14)References (63)

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Western blot - Anti-Estrogen Receptor beta antibody (ab3576)
  • Immunocytochemistry - Anti-Estrogen Receptor beta antibody (ab3576)
  • Western blot - Anti-Estrogen Receptor beta antibody (ab3576)
  • Immunocytochemistry - Anti-Estrogen Receptor beta antibody (ab3576)

Key features and details

  • Rabbit polyclonal to Estrogen Receptor beta
  • Suitable for: ICC, ICC/IF, WB
  • Reacts with: Human, Chinese hamster
  • Isotype: IgG

You may also be interested in

Biochemical
DPN, ERbeta agonist (ab120166)
Protein
Product image
Recombinant Human Estrogen Receptor beta protein (ab158385)
Primary
Product image
Anti-Estrogen Receptor alpha antibody [TE111.5D11] (ab16460)

View more associated products

Overview

  • Product name

    Anti-Estrogen Receptor beta antibody
    See all Estrogen Receptor beta primary antibodies
  • Description

    Rabbit polyclonal to Estrogen Receptor beta
  • Host species

    Rabbit
  • Specificity

    By Western blot, this antibody detects an ~55 kDa protein representing ER beta from Rat brain homogenate. Immunocytochemical staining of ER beta in cells overexpressing the Human protein results in nuclear staining.
  • Tested Applications & Species

    Application Species
    ICC
    Human
    Chinese hamster
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Rat Estrogen Receptor beta aa 467-485.
    Sequence:

    CSSTEDSKNKESSQNLQSQ


    (Peptide available as ab5018)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Estrogen
    • Neuroscience
    • Endocrine system
    • Gonadotrophic axis
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Estrogen
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • Nuclear hormone receptors
    • Estrogen
    • Cancer
    • Tumor biomarkers
    • Receptors
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Metabolic disorders

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Estrogen Receptor beta protein (ab158385)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab3576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC
Human
Chinese hamster
WB
Human
All applications
Hamster
Application Abreviews Notes
ICC
1/100 - 1/1000.
ICC/IF
1/100 - 1/1000.
WB (2)
Use at an assay dependent concentration. Predicted molecular weight: 59 kDa.
Notes
ICC
1/100 - 1/1000.
ICC/IF
1/100 - 1/1000.
WB
Use at an assay dependent concentration. Predicted molecular weight: 59 kDa.

Target

  • Function

    Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.
  • Tissue specificity

    Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q92731 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2100 Human
    • Omim: 601663 Human
    • SwissProt: Q92731 Human
    • Unigene: 660607 Human
    • Unigene: 729020 Human
    • Alternative names

      • ER BETA antibody
      • ER-beta antibody
      • Erb antibody
      • ESR B antibody
      • ESR BETA antibody
      • ESR2 antibody
      • ESR2_HUMAN antibody
      • ESRB antibody
      • ESTRB antibody
      • estrogen nuclear receptor beta variant a antibody
      • estrogen nuclear receptor beta variant b antibody
      • estrogen receptor 2 (ER beta) antibody
      • Estrogen receptor 2 antibody
      • estrogen receptor beta 4 antibody
      • Estrogen receptor beta antibody
      • NR3A2 antibody
      • Nuclear receptor subfamily 3 group A member 2 antibody
      see all

    Images

    • Western blot - Anti-Estrogen Receptor beta antibody (ab3576)
      Western blot - Anti-Estrogen Receptor beta antibody (ab3576)
      All lanes : Anti-Estrogen Receptor beta antibody (ab3576) at 1/1000 dilution

      Lane 1 : MCF7 whole cell lysates
      Lane 2 : MCF7 nuclear extract
      Lane 3 : T47D whole cell lysates
      Lane 4 : T47D nuclear extract
      Lane 5 : SK-BR-3 whole cell lysates
      Lane 6 : SK-BR-3 nuclear extract
      Lane 7 : Hs 578T whole cell lysates
      Lane 8 : Hs 578T nuclear extract

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG at 1/40000 dilution

      Predicted band size: 59 kDa



      HDAC1 polyclonal antibody (bottom panel) at a dilution of 1/1000 overnight at 4C, followed by an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1/40,000. Chemiluminescent detection.

    • Immunocytochemistry - Anti-Estrogen Receptor beta antibody (ab3576)
      Immunocytochemistry - Anti-Estrogen Receptor beta antibody (ab3576)
      Immunofluorescent analysis using Estrogen Receptor beta polyclonal antibody (ab3576) shows nuclear localization of Estrogen Receptor beta in MCF7 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% BSA for 15 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing Estrogen Receptor beta (ab3576), at a dilution of 1:100 for 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.
    • Western blot - Anti-Estrogen Receptor beta antibody (ab3576)
      Western blot - Anti-Estrogen Receptor beta antibody (ab3576)
      All lanes : Anti-Estrogen Receptor beta antibody (ab3576) at 1/2000 dilution

      Lane 1 : CAL region of Human brain - Whole cell lysate
      Lane 2 : EC region of Human brain - Whole cell lysate
      Lane 3 : HIP region of Human brain - Whole cell lysate
      Lane 4 : IT region of Human brain - Whole cell lysate
      Lane 5 : MF region of Human brain - Whole cell lysate
      Lane 6 : SP region of Human brain - Whole cell lysate

      Secondary
      All lanes : HRP conjugated Donkey Anti-rabbit IgG

      Predicted band size: 59 kDa
      Observed band size: 55 kDa
      why is the actual band size different from the predicted?



      This image is courtesy of an Abreview submitted on 3 February 2006.

      See Abreview

    • Immunocytochemistry - Anti-Estrogen Receptor beta antibody (ab3576)
      Immunocytochemistry - Anti-Estrogen Receptor beta antibody (ab3576)

      Immunolocalization of ER beta from CHO cells overexpressing the Human protein, using ab3576 (1ug/ml).

    Protocols

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (63)

    Publishing research using ab3576? Please let us know so that we can cite the reference in this datasheet.

    ab3576 has been referenced in 63 publications.

    • Skórkowska A  et al. Effect of Combined Prenatal and Adult Benzophenone-3 Dermal Exposure on Factors Regulating Neurodegenerative Processes, Blood Hormone Levels, and Hematological Parameters in Female Rats. Neurotox Res 37:683-701 (2020). PubMed: 31970650
    • Yao Q  et al. Aloe/poloxamer hydrogel as an injectable ß-estradiol delivery scaffold with multi-therapeutic effects to promote endometrial regeneration for intrauterine adhesion treatment. Eur J Pharm Sci 148:105316 (2020). PubMed: 32201342
    • Son HJ  et al. 17ß-Estradiol reduces inflammation and modulates antioxidant enzymes in colonic epithelial cells. Korean J Intern Med 35:310-319 (2020). PubMed: 30336658
    • Wang S  et al. Effects of High-Glucose and High-Fat Condition on Estrogen Receptor- and Sexual Precocity-Related Genes in GT1-7 Cells. Med Sci Monit 26:e922860 (2020). PubMed: 32451371
    • Le Naour A  et al. EO771, the first luminal B mammary cancer cell line from C57BL/6 mice. Cancer Cell Int 20:328 (2020). PubMed: 32699527
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 22 Abreviews or Q&A

    Question

    Inquiry: Please specify which antigen retrieval buffer is recommended to use with ab3576? Thank you.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 14 2013

    Answer

    Thank you for your enquiry and interest in our products.

    I can confirm that ab3576: Anti-Estrogen Receptor beta has been used in different dilutions in IHC (P).

    There are two protocols which were used the same buffer but slightly different temperature and both were successfully applied with this antibody:

    A) Paraffin-embedded sections were dewaxed and rehydrated through graded alcohol. Heat-mediated antigen retrieval was performed at 100 C in 1 liter 0.01 M citrate buffer (pH 6.0). Concentration of antibody used: 5 µg/ml.

    B) After washing, the sections were transferred to plastic jars containing 0.01 M citrate buffer (pH 6.0) and boiled in a microwave oven for 10 min at 700 W. Samples were allowed to cool for 20 min before washing in PBS. 1:500 dilution in PBS + 2% BSA.

    If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Feb 14 2013

    Question

    Catalog number(s): 3576
    Product(s): Rabbit polyclonal to Estrogen Receptor beta

    -----------------------------------
    Telephone section
    Q. Did you contact Abcam's Scientific Support by telephone in reference to this complaint?
    A. Yes
    Q. Was it clear from the phone options which number you needed to press to get your issue resolved?
    A. Yes

    Protocol advice section
    Q. Did Abcam's Scientific Support give you protocol advice?
    A.Yes
    Q. How did you feel about being given protocol advice?
    A.I was not expecting to be given protocol advice, but was happy to receive this
    Q. Did the product work successfully after following the advice?
    A.No
    Q. Why have you not tried the protocol advice? (Select all that apply.)
    A.

    Free of charge replacement section
    Q. Did Abcam's Scientific Support give you a free of charge replacement?
    A. Yes

    Q. Did the replacement product work successfully?
    A.Yes
    Credit note / refund section
    Q. Did Abcam's Scientific Support give you a credit note or a refund?
    A.Yes

    Q. Would you have preferred a different outcome from receiving a credit note or refund?
    A.No
    Overall satisfaction section
    Q. How satisfied are you with how the complaint was handled?
    A.Very satisfied
    Q. Could we have done anything to better resolve the problem?
    A.
    Q. Would you mind if our scientific support team contacted you to better understand what happened and reach an agreeable solution?
    A.No, I would not mind
    Q. Please share with us any final thoughts you may have about Abcam, the complaints process, or our products.
    A.Not this time. Thanks.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 05 2012

    Answer

    Thank you for your participation in Abcam's post complaint survey.

    Please let me know if there is anything else I can do for you, or if you have questions to any of our products (e.g. primary antibodies, lysates, secondary antibodies, kits, controls, gels, proteins/peptides, slides, biochemicals, conjugation kits).

    Read More

    Abcam Scientific Support

    Answered on Jul 05 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Estrogen Receptor beta antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated
    Permeabilization
    No
    Specification
    Kidney
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% normal buffered formalin
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 09 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Estrogen Receptor beta antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Monkey Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TE pH9
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% normal buffered formalin
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 09 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Estrogen Receptor beta antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (Lung)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TE pH9
    Permeabilization
    No
    Specification
    Lung
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% normal buffered formalin
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 09 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Estrogen Receptor beta antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Breast)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TE pH9
    Permeabilization
    No
    Specification
    Breast
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    10% normal buffered formalin
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 09 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Estrogen Receptor beta antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TE pH9
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% normal buffered formalin
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 09 2019

    Flow Cytometry abreview for Anti-Estrogen Receptor beta antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Rat Cell (Lung tissue)
    Permeabilization
    Yes - eBiosciences FoxP3 intracellular staining buffer set
    Gating Strategy
    Leukocytes
    Specification
    Lung tissue
    Preparation
    Cell harvesting/tissue preparation method: Lungs were dissected and digested using Collagenase IV+DNase
    Sample buffer: GKN+10% FCS
    Fixation
    eBiosciences FoxP3 intracellular staining buffer set
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 12 2016

    Question

    Inquiry: Got multiple bands, not possible to know which bands corresponds to ERbeta 20 and 50 ug of whole cell lysates from MCF7 and RI-1 cells were loaded. Blocking conditions: 5% milk for 2 hours Primary antibody conditions: 1:1000 ON at 4deg in TBS-T 3 times 10' wash in TBS-T Secundary antibody conditions (no Abcam): 1:5000 1h at RT in TBS-T 3 times 10' wash in TBS-T Detection methods: ECL (ThermoFisher) Any suggestions? Would a antibody replacement possible? Thanks Best wishes

    Read More

    Abcam community

    Verified customer

    Asked on Nov 06 2012

    Answer

    Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.
    The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.
    I would like to reassure you that ab3576 is tested and covered by our 6 month guarantee for use in WB and human, mouse and rat samples. In the event that a product is not functioning in the applications cited on the product data sheet, we will be pleased to provide a credit note or free of charge replacement.
    Reviewing this case, I would like to offer some suggestions to help optimise the results from ab3576.
    1.) Antibodies will produce high background if they are used too concentrated. I suggest to further dilute the antibody.
    2.) background is often generated by the secondary antibody and therefore I can recommend to rum a "no primary" control.
    3.) Cell lines often produce artifact due to prolonged culturing. We have reports from customers using this antibody in tissue samples with good results. I suggest to run a positive control with a suitable tissue lysate.
    4.) Since Estrogen Receptor beta is a nuclear protein, I can suggest to prepare nuclear lysates to clean up the blot.
    I would also appreciate if you can confirm some further details:
    1.) What lysis buffer was used?
    2.) There are 8 isoforms of this protein. They range from 35kDa to 59kDa. Do you which one are expressed in the cell lines used?
    I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.

    Read More

    Abcam Scientific Support

    Answered on Nov 06 2012

    Question


    I believe I would like to wait for the ab3576 to become available. If it is not available by the end of August, then I would like to try the ab5784 antibody.
    Thanks so much for you help.

    Read More

    Abcam community

    Verified customer

    Asked on Aug 10 2012

    Answer

    Thank you very much for you reply.

    I'm sending a free of charge vial of ab3576 on the order ***, which we expect to arrive around August 24. Your purchasing agent will receive an email update if there are further delays to expect.

    Please keep me updated about the results with this new antibody, and let me know if there is anything else that we can do for you. I look forward to hearing from you.

    Have a nice day!

    Read More

    Abcam Scientific Support

    Answered on Aug 10 2012

    1-10 of 22 Abreviews or Q&A

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