Anti-Estrogen Receptor beta antibody (ab3576)
Key features and details
- Rabbit polyclonal to Estrogen Receptor beta
- Suitable for: ICC, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Estrogen Receptor beta antibody
See all Estrogen Receptor beta primary antibodies -
Description
Rabbit polyclonal to Estrogen Receptor beta -
Host species
Rabbit -
Specificity
By Western blot, this antibody detects an ~55 kDa protein representing ER beta from Rat brain homogenate. Immunocytochemical staining of ER beta in cells overexpressing the Human protein results in nuclear staining. -
Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Hamster -
Immunogen
Synthetic peptide corresponding to Rat Estrogen Receptor beta aa 467-485.
Sequence:CSSTEDSKNKESSQNLQSQ
(Peptide available asab5018) -
Positive control
- WB: MCF7 whole cell lysate; T47and SK-BR3 nuclear extracts
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
1/100 - 1/1000.
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WB | (2) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 59 kDa.
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Notes |
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ICC
1/100 - 1/1000. |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 59 kDa. |
Target
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Function
Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual. -
Tissue specificity
Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2100 Human
- Omim: 601663 Human
- SwissProt: Q92731 Human
- Unigene: 660607 Human
- Unigene: 729020 Human
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Alternative names
- ER BETA antibody
- ER-beta antibody
- Erb antibody
see all
Images
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All lanes : Anti-Estrogen Receptor beta antibody (ab3576) at 1/1000 dilution
Lane 1 : MCF7 whole cell lysates
Lane 2 : MCF7 nuclear extract
Lane 3 : T47D whole cell lysates
Lane 4 : T47D nuclear extract
Lane 5 : SK-BR-3 whole cell lysates
Lane 6 : SK-BR-3 nuclear extract
Lane 7 : Hs 578T whole cell lysates
Lane 8 : Hs 578T nuclear extract
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/40000 dilution
Predicted band size: 59 kDaHDAC1 polyclonal antibody (bottom panel) at a dilution of 1/1000 overnight at 4C, followed by an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1/40,000. Chemiluminescent detection.
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Immunofluorescent analysis using Estrogen Receptor beta polyclonal antibody (ab3576) shows nuclear localization of Estrogen Receptor beta in MCF7 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% BSA for 15 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing Estrogen Receptor beta (ab3576), at a dilution of 1:100 for 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (94)
ab3576 has been referenced in 94 publications.
- Skibińska I et al. Expression of estrogen receptors, PELP1, and SRC in human spermatozoa and their associations with semen quality. Hum Cell 36:554-567 (2023). PubMed: 36577884
- Park SA et al. Inhibition of GP130/STAT3 and EMT by combined bazedoxifene and paclitaxel treatment in ovarian cancer. Oncol Rep 47:N/A (2022). PubMed: 35029286
- Wang B et al. Ahi1 regulates serotonin production by the GR/ERβ/TPH2 pathway involving sexual differences in depressive behaviors. Cell Commun Signal 20:74 (2022). PubMed: 35643536
- Piperigkou Z et al. ESR2 Drives Mesenchymal-to-Epithelial Transition in Triple-Negative Breast Cancer and Tumorigenesis In Vivo. Front Oncol 12:917633 (2022). PubMed: 35719919
- Zeng M et al. A sesquiterpene isolated from the stems and leaves of Dioscorea opposita thunb. Transforms the composition of immune cells through ERβ in a mouse model of LPS-induced lung injury. Heliyon 8:e10500 (2022). PubMed: 36105471