• Product name
    Anti-Estrogen Receptor beta antibody
    See all Estrogen Receptor beta primary antibodies
  • Description
    Rabbit polyclonal to Estrogen Receptor beta
  • Host species
  • Specificity
    By Western blot, this antibody detects an ~55 kDa protein representing ER beta from Rat brain homogenate. Immunocytochemical staining of ER beta in cells overexpressing the Human protein results in nuclear staining.
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, ICC, WB, Inhibition Assay, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Human, Pig, Non human primates
    Predicted to work with: Hamster
  • Immunogen

    Synthetic peptide corresponding to Rat Estrogen Receptor beta aa 467-485.


    (Peptide available as ab5018)

  • Positive control
    • WB: Rat brain lysate IHC: testis, efferent ductules, epididymis, and vas deferens (see Zhou et al), Rat ovary (see Sanchez-Criado)



Our Abpromise guarantee covers the use of ab3576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
IHC-Fr 1/50.
ICC 1/100 - 1/1000.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 59 kDa).Can be blocked with Estrogen Receptor beta peptide (ab5018).
Inhibition Assay Use at an assay dependent concentration.
ICC/IF 1/100 - 1/1000.


  • Function
    Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.
  • Tissue specificity
    Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain
    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • ER BETA antibody
    • ER-beta antibody
    • Erb antibody
    • ESR B antibody
    • ESR BETA antibody
    • ESR2 antibody
    • ESR2_HUMAN antibody
    • ESRB antibody
    • ESTRB antibody
    • estrogen nuclear receptor beta variant a antibody
    • estrogen nuclear receptor beta variant b antibody
    • estrogen receptor 2 (ER beta) antibody
    • Estrogen receptor 2 antibody
    • estrogen receptor beta 4 antibody
    • Estrogen receptor beta antibody
    • NR3A2 antibody
    • Nuclear receptor subfamily 3 group A member 2 antibody
    see all


  • Immunofluorescence analysis of frozen murine thoracic aorta, staining Estrogen Receptor beta with ab3576 at 1/50 dilution (green: not merged with nucleus, or purple: merged with nucleus). Nuclei were stained with DAPI (blue) and actin fibers were stained red.
  • Immunofluorescent analysis using Estrogen Receptor beta polyclonal antibody (ab3576) shows nuclear localization of Estrogen Receptor beta in MCF7 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% BSA for 15 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing Estrogen Receptor beta (ab3576), at a dilution of 1:100 for 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.
  • All lanes : Anti-Estrogen Receptor beta antibody (ab3576) at 1/2000 dilution

    Lane 1 : CAL region of Human brain - Whole cell lysate
    Lane 2 : EC region of Human brain - Whole cell lysate
    Lane 3 : HIP region of Human brain - Whole cell lysate
    Lane 4 : IT region of Human brain - Whole cell lysate
    Lane 5 : MF region of Human brain - Whole cell lysate
    Lane 6 : SP region of Human brain - Whole cell lysate

    All lanes : HRP conjugated Donkey Anti-rabbit IgG

    Predicted band size: 59 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?

    This image is courtesy of an Abreview submitted on 3 February 2006.

    See Abreview

  • Immunolocalization of ER beta from CHO cells overexpressing the Human protein, using ab3576 (1ug/ml).


This product has been referenced in:
  • Zhou F  et al. Raloxifene, a promising estrogen replacement, limits TDP-25 cell death by enhancing autophagy and suppressing apoptosis. Brain Res Bull 140:281-290 (2018). Read more (PubMed: 29807145) »
  • Wang J  et al. G-1 exhibit antidepressant effect, increase of hippocampal ERs expression and improve hippocampal redox status in aged female rats. Behav Brain Res N/A:N/A (2018). Read more (PubMed: 30041006) »
See all 43 Publications for this product

Customer reviews and Q&As

1-10 of 14 Q&A


Thank you for your enquiry and interest in our products.

I can confirm that ab3576: Anti-Estrogen Receptor beta has been used in different dilutions in IHC (P).

There are two protocols which were used the same buffer but slightly different temperature and both were successfully applied with this antibody:

A) Paraffin-embedded sections were dewaxed and rehydrated through graded alcohol. Heat-mediated antigen retrieval was performed at 100 C in 1 liter 0.01 M citrate buffer (pH 6.0). Concentration of antibody used: 5 µg/ml.

B) After washing, the sections were transferred to plastic jars containing 0.01 M citrate buffer (pH 6.0) and boiled in a microwave oven for 10 min at 700 W. Samples were allowed to cool for 20 min before washing in PBS. 1:500 dilution in PBS + 2% BSA.

If you need any further assistance in the future, please do not hesitate to contact me.

Read More


Catalog number(s): 3576
Product(s): Rabbit polyclonal to Estrogen Receptor beta

Telephone section
Q. Did you contact Abcam's Scientific Support by telephone in reference to this complaint?
A. Yes
Q. Was it clear from the phone options which number you needed to press to get your issue resolved?
A. Yes

Protocol advice section
Q. Did Abcam's Scientific Support give you protocol advice?
Q. How did you feel about being given protocol advice?
A.I was not expecting to be given protocol advice, but was happy to receive this
Q. Did the product work successfully after following the advice?
Q. Why have you not tried the protocol advice? (Select all that apply.)

Free of charge replacement section
Q. Did Abcam's Scientific Support give you a free of charge replacement?
A. Yes

Q. Did the replacement product work successfully?
Credit note / refund section
Q. Did Abcam's Scientific Support give you a credit note or a refund?

Q. Would you have preferred a different outcome from receiving a credit note or refund?
Overall satisfaction section
Q. How satisfied are you with how the complaint was handled?
A.Very satisfied
Q. Could we have done anything to better resolve the problem?
Q. Would you mind if our scientific support team contacted you to better understand what happened and reach an agreeable solution?
A.No, I would not mind
Q. Please share with us any final thoughts you may have about Abcam, the complaints process, or our products.
A.Not this time. Thanks.

Read More

Thank you for your participation in Abcam's post complaint survey.

Please let me know if there is anything else I can do for you, or if you have questions to any of our products (e.g. primary antibodies, lysates, secondary antibodies, kits, controls, gels, proteins/peptides, slides, biochemicals, conjugation kits).

Read More


Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.
The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.
I would like to reassure you that ab3576 is tested and covered by our 6 month guarantee for use in WB and human, mouse and rat samples. In the event that a product is not functioning in the applications cited on the product data sheet, we will be pleased to provide a credit note or free of charge replacement.
Reviewing this case, I would like to offer some suggestions to help optimise the results from ab3576.
1.) Antibodies will produce high background if they are used too concentrated. I suggest to further dilute the antibody.
2.) background is often generated by the secondary antibody and therefore I can recommend to rum a "no primary" control.
3.) Cell lines often produce artifact due to prolonged culturing. We have reports from customers using this antibody in tissue samples with good results. I suggest to run a positive control with a suitable tissue lysate.
4.) Since Estrogen Receptor beta is a nuclear protein, I can suggest to prepare nuclear lysates to clean up the blot.
I would also appreciate if you can confirm some further details:
1.) What lysis buffer was used?
2.) There are 8 isoforms of this protein. They range from 35kDa to 59kDa. Do you which one are expressed in the cell lines used?
I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.

Read More


Thank you very much for you reply.

I'm sending a free of charge vial of ab3576 on the order ***, which we expect to arrive around August 24. Your purchasing agent will receive an email update if there are further delays to expect.

Please keep me updated about the results with this new antibody, and let me know if there is anything else that we can do for you. I look forward to hearing from you.

Have a nice day!

Read More


Thank you again for your call yesterday and for letting us know about the trouble with ab3577.

I've checked with our supply chain and we do expect to have ab3576 back in stock by mid-late August, tentatively August 21. Would this work for you?

I look forward to hearing from you. Please let me know if you have any further questions or if there is anything else that I can do for you.

Read More


Thank you very much for your call today and for letting us know about the trouble with ab3577.

As we discussed, I will be happy to send a free of charge replacement antibody since this one did not work. I'm checking with our supply chain regarding the availability of ab3576, and I will get back to you as soon as I have more information. The other antibodies that we discussed are ab78946 and ab5784, which have not been tested in all of the species and applications that you need the antibody for, but theyseem to bethe best candidates that we have in stock at this time.

Please let me know if you have any questions or if there is anything else that we can do in the meantime.

Read More


Thank you for your reply. I am sorry that ab3576 was also producing high background. I have arranged to send you ab3577 as a replacement and am also sending a positive control mouse brain nuclear lysate, which may help to clarify your results. The replacements will ship on order ***. I hope this helps, please let me know if you need any additional assistance.

Read More


Thank you very much for your reply.

My colleague is out of the office today but I am happy to assist you.

I have alerted our shipping department to remove these two phrases from your shipping label. Please let me know if there is anything else that we can do for you.

Have a nice day.

Read More


Thank you for your reply.

I am sorry that ab16813 is still producing multiple bands in WB. I would be happy to send you ab3576 or ab3577 as a free of charge replacement, as we had previously discussed. Please let me know your original order or PO number and which antibody you would like to receive, and I will be happy to assist you further.

Read More


Thank you for contacting us. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:


I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

Read More

1-10 of 14 Q&A


Sign up