Product nameAnti-Estrogen Receptor beta antibody
See all Estrogen Receptor beta primary antibodies
DescriptionRabbit polyclonal to Estrogen Receptor beta
SpecificityBy Western blot, this antibody detects an ~55 kDa protein representing ER beta from Rat brain homogenate. Immunohistochemical staining of ER beta in Rat cerebellum results in cytoplasmic staining in Purkinje neurons. This antibody sufficiently supershifts Rat ER beta / ER response element (vitellogenin xenopus A2 gene).
Tested applicationsSuitable for: ICC/IF, EMSA, IHC-P, IHC-Fr, WB, ICC, ChIP, Flow Cyt, IPmore details
Species reactivityReacts with: Mouse, Rat, Sheep, Hamster, Cow, Human, Pig, Non human primates, Baboon
- WB: Rat brain lysate IHC: Rat cerebellum, rat penis (see Jesmin et al), Ovine uterine artery endothelial cells (see Liao et al) ICC: Human coronary artery smooth muscle cells (see Han et al)
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
ChIP Related Products
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab3577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.5 µg/ml.|
|EMSA||Use at an assay dependent concentration. PubMed: 18790053|
|IHC-P||Use a concentration of 1 - 2 µg/ml.|
|IHC-Fr||Use a concentration of 1 - 2 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 59 kDa).Can be blocked with Human Estrogen Receptor beta peptide (ab3564).|
|ICC||Use a concentration of 2 - 4 µg/ml.|
|ChIP||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
|EMSA||Use a concentration of 1 - 5 µg/ml.|
FunctionNuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.
Tissue specificityIsoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
- Information by UniProt
- ER BETA antibody
- ER-beta antibody
- Erb antibody
ab3577 staining Estrogen Receptor beta in human placental sac tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde, cut into 20 micron slices, blocked with 2.5% serum for 30 minutes at 25°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/200 in PBS) at 25°C for 90 minutes. An undiluted HRP-conjugated horse polyclonal was used as the secondary antibody.
ab3577 labelling Estrogen Receptor beta by Immunocytochemistry/Immunofluorescence.
ab3577 at a concentration of 1-2µg/ml staining ER beta in primate amygdala.
Red - ab3577 at a 1/100 dilution
Green - Actin loading control
All samples loaded as 20µl.
From left->right 1. Biorad ladder 2. B.end3 cells 20ug 3. B.end3 cells 40ug 4. B.end3 cells 60ug 5. B.end3 cells 80ug 6. Biorad ladder 7. Mouse Uterus Homogenate 10ug (+ Control) 8. Mouse Uterus Homogenate 20ug (+ Control) 9. Mouse Uterus Homogenate 40ug (+ Control) 10. Biorad Ladder
The secondary used was an IRDye700DX conjugated rabbit polyclonal used at a 1/10000 dilution.
This product has been referenced in:
- Huo Y et al. Tph2-/- female mice restore socio-sexual recognition through upregulating ERa and OTR genes in the amygdala. PLoS One 13:e0193395 (2018). Read more (PubMed: 29470551) »
- Lorsch ZS et al. Estrogen receptor a drives pro-resilient transcription in mouse models of depression. Nat Commun 9:1116 (2018). Read more (PubMed: 29549264) »