Overview

  • Product name
    Anti-Estrogen Receptor beta antibody
    See all Estrogen Receptor beta primary antibodies
  • Description
    Rabbit polyclonal to Estrogen Receptor beta
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human, Pig, Lizard, Non human primates, Baboon
  • Immunogen

    Synthetic peptide corresponding to Human Estrogen Receptor beta aa 459-477.
    Sequence:

    CSPAEDSKSKEGSQNPQSQ


    (Peptide available as ab41764)

  • Positive control
    • WB: rat brain lysate IHC: rat prostate

Properties

Applications

Our Abpromise guarantee covers the use of ab5786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 59 kDa).Can be blocked with Human Estrogen Receptor beta peptide (ab41764).
ICC Use at an assay dependent concentration.
IHC-Fr Use a concentration of 2 - 5 µg/ml.
IHC-P Use a concentration of 2 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.
  • Tissue specificity
    Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain
    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ER BETA antibody
    • ER-beta antibody
    • Erb antibody
    • ESR B antibody
    • ESR BETA antibody
    • ESR2 antibody
    • ESR2_HUMAN antibody
    • ESRB antibody
    • ESTRB antibody
    • estrogen nuclear receptor beta variant a antibody
    • estrogen nuclear receptor beta variant b antibody
    • estrogen receptor 2 (ER beta) antibody
    • Estrogen receptor 2 antibody
    • estrogen receptor beta 4 antibody
    • Estrogen receptor beta antibody
    • NR3A2 antibody
    • Nuclear receptor subfamily 3 group A member 2 antibody
    see all

Images

  • Immunohistochemical staining with ab5786 detects nuclear Estrogen Receptor beta in rat prostate

References

This product has been referenced in:
  • Li Y  et al. Cell sex affects extracellular matrix protein expression and proliferation of smooth muscle progenitor cells derived from human pluripotent stem cells. Stem Cell Res Ther 8:156 (2017). Read more (PubMed: 28676082) »
  • Peng J  et al. Expression of voltage-gated sodium channel Nav1.5 in non-metastatic colon cancer and its associations with estrogen receptor (ER)-ß expression and clinical outcomes. Chin J Cancer 36:89 (2017). IHC-P ; Human . Read more (PubMed: 29122010) »
See all 4 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for your inquiry. My colleague will send you a proforma invoice shortly.

In regards to your question of staining: One vial of ab5786 contains 50ul. The optimal dilution should be determined in your laboratory. Lets suppose you will have a nice staining with a dilution of 1/50, you would have 2.5ml of staining solution. If the antibody does work well with a dilution of 1/100 in your hands, you will have 5ml of staining solution.

Depending on the size of your slide, you will need to use either 50ul or 100ul of staining solution per slide. If it is a big tissue on the slide, it might even could be 200ul. I recommend to check what volume is optimal to cover well the tissue without risk of drying.
If you would need 100ul of staining solution per slide, you would need 20ml of staining solution for 200 slides.

I am sorry that I can not give you a definite answer. It depends on the final dilution factor in your hands as well as on the size of the tissue.

I hope this information is nevertheless helpful. Please do not hesitate to contact us again, should you have any additional question.

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human breast cancer xenograft(MCF-7))
Specification
Human breast cancer xenograft(MCF-7)
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH7.0
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 17 2010

Answer

Thank you for your enquiry. I'm sorry ab5786 is giving your customer difficulty. From the protocol you give me I think the antigen retrieval method is fine and the incubation time/temperature for the primary antibody is fine too. I would check whether the buffers used to dilute the antibodies contain triton 0.3%v/v, as this greatly helps the penetration of the antibody in the sections. Does your customer have a positive control? Does his secondary antibody work with other antibodies in the lab, or could it have a problem? Please let me know if you need further advice, I hope this helps,

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