Anti-Estrogen Receptor beta (phospho S105) antibody (ab62257)

Rabbit polyclonal Estrogen Receptor beta (phospho S105) antibody. Validated in WB, ELISA, IHC, ICC/IF and tested in Human. Cited in 2 publication(s). Independently reviewed in 1 review(s).

Overview

  • Product name

    Anti-Estrogen Receptor beta (phospho S105) antibody
    See all Estrogen Receptor beta primary antibodies
  • Description

    Rabbit polyclonal to Estrogen Receptor beta (phospho S105)
  • Host species

    Rabbit
  • Specificity

    ab62257 detects endogenous levels of Estrogen Receptor beta only when phosphorylated at serine 105.
  • Tested applications

    Suitable for: IHC-P, WB, ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human Estrogen Receptor beta around the phosphorylation site of serine 105 (Q-K-SP-P-W).

  • Positive control

    • HuvEc cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab62257 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 20696772
WB Use at an assay dependent concentration. PubMed: 20696772
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

Target

  • Function

    Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.
  • Tissue specificity

    Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • ER BETA antibody
    • ER-beta antibody
    • Erb antibody
    • ESR B antibody
    • ESR BETA antibody
    • ESR2 antibody
    • ESR2_HUMAN antibody
    • ESRB antibody
    • ESTRB antibody
    • estrogen nuclear receptor beta variant a antibody
    • estrogen nuclear receptor beta variant b antibody
    • estrogen receptor 2 (ER beta) antibody
    • Estrogen receptor 2 antibody
    • estrogen receptor beta 4 antibody
    • Estrogen receptor beta antibody
    • NR3A2 antibody
    • Nuclear receptor subfamily 3 group A member 2 antibody
    see all

Images

  • ab62257 at 1/500 - 1/1000 dilution staining Estrogen Receptor beta in HuvEc cells by Immunofluorescence, in the absence or presence of the immunising peptide

References

This product has been referenced in:

  • Qin L  et al. Luteolin-7-O-glucoside protects dopaminergic neurons by activating estrogen-receptor-mediated signaling pathway in MPTP-induced mice. Toxicology 426:152256 (2019). Read more (PubMed: 31381935) »
  • Adelöf J  et al. PA28aß overexpression enhances learning and memory of female mice without inducing 20S proteasome activity. BMC Neurosci 19:70 (2018). Read more (PubMed: 30400847) »
See all 4 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain (choroid plexus))
Permeabilization
Yes - Triton X-100
Specification
Brain (choroid plexus)
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Formaldehyde

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Verified customer

Submitted Jan 09 2018

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