Anti-Estrogen Related Receptor alpha antibody (ab150560)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Estrogen Related Receptor alpha antibody
See all Estrogen Related Receptor alpha primary antibodies -
Description
Rabbit polyclonal to Estrogen Related Receptor alpha -
Host species
Rabbit -
Specificity
BLAST analysis of the peptide immunogen showed no homology with other Human proteins, except Estrogen Related Receptor gamma (60%). -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Hamster, Cow, Dog, Pig, Monkey, Gorilla
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Immunogen
Synthetic peptide corresponding to Human Estrogen Related Receptor alpha. Synthetic peptide, corresponding to 15 amino acids from ligand-binding domain of Human Estrogen Related Receptor alpha.
Database link: P11474 -
Positive control
- Human breast carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab150560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 11 - 21 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Binds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. Can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. Binds to the C1 region of the lactoferrin gene promoter. Requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Post-translational
modificationsPhosphorylation on Ser-19 enhances sumoylation on Lys-14 increasing repression of transcriptional activity.
Sumoylated by SUMO2. Main site is Lys-14 which is enhanced by phosphorylation on Ser-19, cofactor activation, and by interaction with PIAS4. Sumoylation enhances repression of transcriptional activiy, but has no effect on subcellular location nor on DNA binding. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 507834 Cow
- Entrez Gene: 403169 Dog
- Entrez Gene: 2101 Human
- Entrez Gene: 26379 Mouse
- Entrez Gene: 100322868 Pig
- Entrez Gene: 293701 Rat
- Omim: 601998 Human
- SwissProt: Q6QMY5 Dog
see all -
Alternative names
- Err 1 antibody
- ERR a antibody
- ERR alpha antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Related Receptor alpha antibody (ab150560)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue, labeling Estrogen Related Receptor alpha using ab150560 at 21 µg/ml.
Protocols
References
ab150560 has not yet been referenced specifically in any publications.
