Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free (ab239879)

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Western blot - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free (ab239879)
  • Western blot - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free (ab239879)
  • Flow Cytometry - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free (ab239879)
  • Immunoprecipitation - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free (ab239879)
  • Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free (ab239879)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR46Y] to Estrogen Related Receptor alpha - BSA and Azide free
  • Suitable for: Flow Cyt, IP, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free
    See all Estrogen Related Receptor alpha primary antibodies

  • Description

    Rabbit monoclonal [EPR46Y] to Estrogen Related Receptor alpha - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab239879 is the carrier-free version of ab76228.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab239879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
1/20.
IP
1/20.
WB
Use at an assay dependent concentration. Predicted molecular weight: 55 kDa.
Notes
Flow Cyt
1/20.
IP
1/20.
WB
Use at an assay dependent concentration. Predicted molecular weight: 55 kDa.

Target

  • Function

    Binds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. Can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. Binds to the C1 region of the lactoferrin gene promoter. Requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism.

  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.

  • Post-translational
    modifications

    Phosphorylation on Ser-19 enhances sumoylation on Lys-14 increasing repression of transcriptional activity.
    Sumoylated by SUMO2. Main site is Lys-14 which is enhanced by phosphorylation on Ser-19, cofactor activation, and by interaction with PIAS4. Sumoylation enhances repression of transcriptional activiy, but has no effect on subcellular location nor on DNA binding.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Err 1 antibody
    • ERR a antibody
    • ERR alpha antibody
    • ERR-alpha antibody
    • Err1 antibody
    • ERR1 protein antibody
    • ERR1_HUMAN antibody
    • ERRa antibody
    • ERRalpha antibody
    • ESRL 1 antibody
    • ESRL1 antibody
    • ESRR A antibody
    • Esrra antibody
    • Estrogen receptor like 1 antibody
    • Estrogen receptor related 1 antibody
    • estrogen receptor related receptor alpha antibody
    • Estrogen receptor-like 1 antibody
    • Estrogen related receptor alpha antibody
    • Estrogen-related receptor alpha antibody
    • Estrra antibody
    • hERR1 antibody
    • NR3B1 antibody
    • Nuclear receptor subfamily 3 group B member 1 antibody
    • Steroid hormone receptor ERR1 antibody
    see all

Images

  • Western blot - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free (ab239879)
    Western blot - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free (ab239879)
    All lanes : Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228) at 1/1000 dilution (Purified)

    Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 55 kDa
    Observed band size: 55 kDa



    This data was developed using ab76228 the same antibody clone in a different buffer formulation.

  • Western blot - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free (ab239879)
    Western blot - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free (ab239879)
    Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228) at 1/1000 dilution (Purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 55 kDa
    Observed band size: 55 kDa



    This data was developed using ab76228 the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free (ab239879)
    Flow Cytometry - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free (ab239879)

    This data was developed using ab76228 the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% Paraformaldehyde fixed 90% Methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Estrogen Receptor alpha with ab76228 at 1/20 dilution (10 µg/ml) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

  • Immunoprecipitation - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free (ab239879)
    Immunoprecipitation - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free (ab239879)

    ab76228 used at 1:20 dilution (2μg) immunoprecipitating Estrogen Related Receptor alpha in Hela whole cell lysates.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg.
    Lane 2 (+): HeLa whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab190685 in HeLa whole cell lysate.

    For western blotting, ab227648 and VeriBlot for IP Detection Reagent (HRP)(ab131366) at 1/1000 dilution were used.

  • Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free (ab239879)
    Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free (ab239879)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab239879? Please let us know so that we can cite the reference in this datasheet.

ab239879 has not yet been referenced specifically in any publications.

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