Recombinant Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free (ab239879)
![Western blot - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free (ab239879)](https://www.abcam.com/ps/products/239/ab239879/Images/ab239879-483447-ab76228296557ab7622820171215WBKOEWAR.jpg "Western blot - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free (ab239879)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR46Y] to Estrogen Related Receptor alpha - BSA and Azide free
- Suitable for: Flow Cyt, IP, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free
See all Estrogen Related Receptor alpha primary antibodies -
Description
Rabbit monoclonal [EPR46Y] to Estrogen Related Receptor alpha - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
ab239879 is the carrier-free version of ab76228.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR46Y -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab239879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/20.
|
|
| IP |
1/20.
|
|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 55 kDa.
|
| Notes |
|---|
|
Flow Cyt
1/20. |
|
IP
1/20. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 55 kDa. |
Target
-
Function
Binds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. Can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. Binds to the C1 region of the lactoferrin gene promoter. Requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Post-translational
modificationsPhosphorylation on Ser-19 enhances sumoylation on Lys-14 increasing repression of transcriptional activity.
Sumoylated by SUMO2. Main site is Lys-14 which is enhanced by phosphorylation on Ser-19, cofactor activation, and by interaction with PIAS4. Sumoylation enhances repression of transcriptional activiy, but has no effect on subcellular location nor on DNA binding. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 2101 Human
- Entrez Gene: 26379 Mouse
- Entrez Gene: 293701 Rat
- Omim: 601998 Human
- SwissProt: P11474 Human
- SwissProt: O08580 Mouse
- SwissProt: Q5QJV7 Rat
- Unigene: 110849 Human
see all -
Alternative names
- Err 1 antibody
- ERR a antibody
- ERR alpha antibody
see all
Images
-
Western blot - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free (ab239879)
This data was developed using ab76228 the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Estrogen Related Receptor alpha knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: MCF7 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab76228 observed at 55 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab76228 was shown to specifically react with Estrogen Related Receptor alpha in wild-type HAP1 cells as signal was lost in Estrogen Related Receptor alpha knockout cells. Wild-type and Estrogen Related Receptor alpha knockout samples were subjected to SDS-PAGE. Ab76228 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
-
![Western blot - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free (ab239879)](https://www.abcam.com/ps/products/239/ab239879/Images/ab239879-468791-anti-estrogen-related-receptor-alpha-antibody-epr46y-westernblot-human-mouse-rat.jpg)
Western blot - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free (ab239879)All lanes : Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228) at 1/1000 dilution (Purified)
Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : Mouse brain lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDaThis data was developed using ab76228 the same antibody clone in a different buffer formulation.
-
![Western blot - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free (ab239879)](https://www.abcam.com/ps/products/239/ab239879/Images/ab239879-468790-anti-estrogen-related-receptor-alpha-antibody-epr46y-westernblot-hela-human.jpg)
Western blot - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free (ab239879)Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228) at 1/1000 dilution (Purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDaThis data was developed using ab76228 the same antibody clone in a different buffer formulation.
-
![Flow Cytometry - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free (ab239879)](https://www.abcam.com/ps/products/239/ab239879/Images/ab239879-4-anti-estrogen-related-receptor-alpha-antibody-epr46y-flowcytometry-hela-human.jpg)
Flow Cytometry - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free (ab239879)This data was developed using ab76228 the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% Paraformaldehyde fixed 90% Methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Estrogen Receptor alpha with ab76228 at 1/20 dilution (10 µg/ml) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.
-
![Immunoprecipitation - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free (ab239879)](https://www.abcam.com/ps/products/239/ab239879/Images/ab239879-468556-anti-estrogen-related-receptor-alpha-antibody-epr46y-immunoprecipitation-hela-human.jpg)
Immunoprecipitation - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free (ab239879)ab76228 used at 1:20 dilution (2μg) immunoprecipitating Estrogen Related Receptor alpha in Hela whole cell lysates.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg.
Lane 2 (+): HeLa whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab190685 in HeLa whole cell lysate.For western blotting, ab227648 and VeriBlot for IP Detection Reagent (HRP)(ab131366) at 1/1000 dilution were used.
-
![Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free (ab239879)](https://www.abcam.com/ps/products/239/ab239879/Images/ab239879-2-benefits-of-recombinant-antibodies.png)
Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free (ab239879)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab239879 has not yet been referenced specifically in any publications.
