Recombinant Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free (ab239879)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR46Y] to Estrogen Related Receptor alpha - BSA and Azide free
- Suitable for: IP, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free
See all Estrogen Related Receptor alpha primary antibodies -
Description
Rabbit monoclonal [EPR46Y] to Estrogen Related Receptor alpha - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details
Unsuitable for: ICC or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Estrogen Related Receptor alpha aa 1-100 (N terminal). The exact sequence is proprietary.
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General notes
Ab239879 is the carrier-free version of ab76228. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab239879 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR46Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab239879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 55 kDa. |
Target
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Function
Binds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. Can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. Binds to the C1 region of the lactoferrin gene promoter. Requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Post-translational
modificationsPhosphorylation on Ser-19 enhances sumoylation on Lys-14 increasing repression of transcriptional activity.
Sumoylated by SUMO2. Main site is Lys-14 which is enhanced by phosphorylation on Ser-19, cofactor activation, and by interaction with PIAS4. Sumoylation enhances repression of transcriptional activiy, but has no effect on subcellular location nor on DNA binding. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2101 Human
- Entrez Gene: 26379 Mouse
- Entrez Gene: 293701 Rat
- Omim: 601998 Human
- SwissProt: P11474 Human
- SwissProt: O08580 Mouse
- SwissProt: Q5QJV7 Rat
- Unigene: 110849 Human
see all -
Alternative names
- Err 1 antibody
- ERR a antibody
- ERR alpha antibody
see all
Images
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![Immunoprecipitation - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free (ab239879)](https://www.abcam.com/ps/products/239/ab239879/Images/ab239879-328491-anti-estrogen-related-receptor-alpha-antibody-epr46y-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free (ab239879)Estrogen Related Receptor alpha was immunoprecipitated using 0.5mg Hela whole cell extract, 10µg of Rabbit monoclonal to Estrogen Related Receptor alpha and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab76228.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: Band: 55kDa: Estrogen Related Receptor alpha.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76228).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab239879 has not yet been referenced specifically in any publications.
