Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free (ab239879)

Overview

  • Product name

    Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free
    See all Estrogen Related Receptor alpha primary antibodies
  • Description

    Rabbit monoclonal [EPR46Y] to Estrogen Related Receptor alpha - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IP, WBmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Estrogen Related Receptor alpha aa 1-100 (N terminal). The exact sequence is proprietary.

  • General notes

    Ab239879 is the carrier-free version of ab76228. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab239879 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab239879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 55 kDa.
  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Function

      Binds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. Can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. Binds to the C1 region of the lactoferrin gene promoter. Requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism.
    • Sequence similarities

      Belongs to the nuclear hormone receptor family. NR3 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • Post-translational
      modifications

      Phosphorylation on Ser-19 enhances sumoylation on Lys-14 increasing repression of transcriptional activity.
      Sumoylated by SUMO2. Main site is Lys-14 which is enhanced by phosphorylation on Ser-19, cofactor activation, and by interaction with PIAS4. Sumoylation enhances repression of transcriptional activiy, but has no effect on subcellular location nor on DNA binding.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • Err 1 antibody
      • ERR a antibody
      • ERR alpha antibody
      • ERR-alpha antibody
      • Err1 antibody
      • ERR1 protein antibody
      • ERR1_HUMAN antibody
      • ERRa antibody
      • ERRalpha antibody
      • ESRL 1 antibody
      • ESRL1 antibody
      • ESRR A antibody
      • Esrra antibody
      • Estrogen receptor like 1 antibody
      • Estrogen receptor related 1 antibody
      • estrogen receptor related receptor alpha antibody
      • Estrogen receptor-like 1 antibody
      • Estrogen related receptor alpha antibody
      • Estrogen-related receptor alpha antibody
      • Estrra antibody
      • hERR1 antibody
      • NR3B1 antibody
      • Nuclear receptor subfamily 3 group B member 1 antibody
      • Steroid hormone receptor ERR1 antibody
      see all

    Images

    • Estrogen Related Receptor alpha was immunoprecipitated using 0.5mg Hela whole cell extract, 10µg of Rabbit monoclonal to Estrogen Related Receptor alpha and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
      The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
      Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab76228.
      Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
      Band: Band: 55kDa: Estrogen Related Receptor alpha.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76228).

    References

    ab239879 has not yet been referenced specifically in any publications.

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