For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    estrogen-related-receptor-beta-antibody-ab19331.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Signaling Pathway Nuclear Signaling Nuclear Hormone Receptors Estrogen
Share by email

Anti-Estrogen Related Receptor beta antibody (ab19331)

  • Datasheet
  • SDS
Reviews (7)Q&A (3)References (10)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-Estrogen Related Receptor beta antibody (ab19331)

    Key features and details

    • Rabbit polyclonal to Estrogen Related Receptor beta
    • Suitable for: WB
    • Reacts with: Rat
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Assay
    Product image
    ALT Assay Kit (384 well, Colorimetric/Fluorometric) (ab241035)
    Primary
    Product image
    Anti-HA tag antibody - ChIP Grade (ab9110)

    View more associated products

    Overview

    • Product name

      Anti-Estrogen Related Receptor beta antibody
    • Description

      Rabbit polyclonal to Estrogen Related Receptor beta
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
      Unsuitable for: ICC/IF
    • Species reactivity

      Reacts with: Rat
    • Immunogen

      Synthetic peptide:

      DYELSQRHEEPRR

      , corresponding to amino acids 376-388 of Mouse Estrogen Related Receptor beta.
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      Preservative: 0.05% Sodium azide
      Constituents: PBS, 0.1% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Signaling Pathway
      • Nuclear Signaling
      • Nuclear Hormone Receptors
      • Estrogen
      • Epigenetics and Nuclear Signaling
      • Nuclear Signaling Pathways
      • Nuclear Receptors
      • Estrogen
      • Cancer
      • Signal transduction
      • Nuclear signaling
      • Nuclear hormone receptors
      • Estrogen
      • Developmental Biology
      • Reproduction
      • Hormones

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab19331 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use a concentration of 2 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
    Notes
    WB
    Use a concentration of 2 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
    Application notes
    Is unsuitable for ICC/IF.

    Target

    • Function

      Nuclear receptor, may regulate ESR1 transcriptional activity.
    • Involvement in disease

      Defects in ESRRB are the cause of deafness autosomal recessive type 35 (DFNB35) [MIM:608565]. DFNB35 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
    • Sequence similarities

      Belongs to the nuclear hormone receptor family. NR3 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession O95718 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 299210 Rat
      • SwissProt: P11475 Rat
      • Unigene: 104984 Rat
      • Alternative names

        • Err 2 antibody
        • ERR b antibody
        • ERR B2 antibody
        • ERR beta 2 antibody
        • ERR beta antibody
        • ERR beta-2 antibody
        • ERR-beta antibody
        • Err2 antibody
        • ERR2_HUMAN antibody
        • ERRB 2 antibody
        • ERRb antibody
        • ERRB2 antibody
        • ERRbeta 2 antibody
        • ERRbeta antibody
        • ESR L2 antibody
        • ESRL 2 antibody
        • ESRL2 antibody
        • Esrrb antibody
        • Estrogen receptor like 2 antibody
        • Estrogen receptor related 2 antibody
        • Estrogen receptor-like 2 antibody
        • Estrogen-related receptor beta antibody
        • Estrrb antibody
        • Nr3b2 antibody
        • Nuclear receptor ERRB2 antibody
        • Nuclear receptor subfamily 3 group B member 2 antibody
        • Orphan nuclear receptor antibody
        • Steroid hormone receptor ERR 2 antibody
        • Steroid hormone receptor ERR2 antibody
        see all

      Images

      • Western blot - Anti-Estrogen Related Receptor beta antibody (ab19331)
        Western blot - Anti-Estrogen Related Receptor beta antibody (ab19331)

        Western blot detection of Estrogen Related Receptor beta from 100 ug of rat kidney lysate (1:500).

      Protocols

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (10)

      Publishing research using ab19331? Please let us know so that we can cite the reference in this datasheet.

      ab19331 has been referenced in 10 publications.

      • Fan YL  et al. A function of fascin1 in the colony formation of mouse embryonic stem cells. Stem Cells N/A:N/A (2020). PubMed: 32379912
      • Hao L  et al. ERR? suppression by Sirt6 alleviates cholestatic liver injury and fibrosis. JCI Insight 5:N/A (2020). PubMed: 32701506
      • Wang Y  et al. ET-1 regulates the human umbilical vein endothelial cell cycle by adjusting the ERß/FOXN1 signaling pathway. Ann Transl Med 8:1499 (2020). PubMed: 33313244
      • Milon A  et al. Do estrogens regulate lipid status in testicular steroidogenic Leydig cell? Acta Histochem 121:611-618 (2019). PubMed: 31126612
      • Pacwa A  et al. Interplay between estrogen-related receptors and steroidogenesis-controlling molecules in adrenals. In vivo and in vitro study. Acta Histochem 120:456-467 (2018). WB ; Rat . PubMed: 29778238
      • Kotula-Balak M  et al. Insights into the role of estrogen-related receptors a, ß and ? in tumor Leydig cells. Tissue Cell 52:78-91 (2018). PubMed: 29857832
      • Milon A  et al. Chlorinated biphenyls effect on estrogen-related receptor expression, steroid secretion, mitochondria ultrastructure but not on mitochondrial membrane potential in Leydig cells. Cell Tissue Res 369:429-444 (2017). PubMed: 28315012
      • Chaudhary S  et al. Overexpression of caspase 7 is ERa dependent to affect proliferation and cell growth in breast cancer cells by targeting p21(Cip). Oncogenesis 5:e219 (2016). ChIP . PubMed: 27089142
      • Kunst S  et al. Photoreceptor cells display a daily rhythm in the orphan receptor Esrrß. Mol Vis 21:173-84 (2015). WB . PubMed: 25737630
      • Collin RW  et al. Mutations of ESRRB encoding estrogen-related receptor beta cause autosomal-recessive nonsyndromic hearing impairment DFNB35. Am J Hum Genet 82:125-38 (2008). IHC-Fr ; Rat . PubMed: 18179891

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-10 of 10 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Estrogen Related Receptor beta antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Rat Tissue sections (Testis)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
      Permeabilization
      Yes - Wash Buffer from Dako with tween
      Specification
      Testis
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      MR. Rudolf Jung

      Verified customer

      Submitted Dec 28 2019

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Estrogen Related Receptor beta antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Testis)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9.0
      Permeabilization
      Yes - Wash Buffer from Dako with tween
      Specification
      Testis
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      MR. Rudolf Jung

      Verified customer

      Submitted Dec 28 2019

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Estrogen Related Receptor beta antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Testis)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
      Permeabilization
      Yes - Wash Buffer from Dako with tween
      Specification
      Testis
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      MR. Rudolf Jung

      Verified customer

      Submitted Nov 21 2019

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Estrogen Related Receptor beta antibody

      Poor
      Abreviews
      Abreviews
      This product is known to not work in this application or species.
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Mouse Cell (mouse embryonic stem cells)
      Permeabilization
      Yes - 0.01% Tween-20, 0.1% Triton-X in PBS
      Specification
      mouse embryonic stem cells
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
      Fixative
      Methanol
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Sep 10 2019

      Immunohistochemistry (Frozen sections) abreview for Anti-Estrogen Related Receptor beta antibody

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (Posterior part of an E9.5 embryo)
      Permeabilization
      Yes - 0.01% Tween-20, 0.1% Triton-X in PBS
      Specification
      Posterior part of an E9.5 embryo
      Blocking step
      Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Malgorzata Borkowska

      Verified customer

      Submitted Jul 24 2019

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Estrogen Related Receptor beta antibody

      Poor
      Abreviews
      Abreviews
      This product is known to not work in this application or species.
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Mouse Cell (Embryonic)
      Permeabilization
      Yes - Triton X-100 (0.5%)
      Specification
      Embryonic
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Oct 22 2015

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Estrogen Related Receptor beta antibody

      Good
      Abreviews
      Abreviews
      This product is known to not work in this application or species.
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Mouse Cell (embryonic stem cell)
      Specification
      embryonic stem cell
      Fixative
      methanol:acetone 1:1
      Permeabilization
      No
      Blocking step
      0.1%BSA and 1% serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Nov 24 2011

      Question

      Dear technical team,
      Our customer would like to purchase ab19331 andstaining mouse ovary.
      He wants to observe difference following ovary developmental.
      Please let me know if it is possible with this antibody.
      Best regards,

      Read More

      Abcam community

      Verified customer

      Asked on Mar 23 2012

      Answer

      Thank you for your enquiry regarding ab19331.

      I can confirm that this antibody has been characterized for IHC-Fr (on frozen tissue), ICC/IF (on cultured cells or cytospin preparations) and in recognizes Estrogen Related Receptor beta in mouse. This receptor is expressed in ovary and the customer may wish to check the following website for further information on the expression profile:

      http://www.ncbi.nlm.nih.gov/UniGene/ESTProfileViewer.cgi?uglist=Mm.235550

      I would advise your customer to do some literature search (PubMed) to find out what sort of studies have been carried out specifically on murine ovary to monitor developmental expression of Estrogen Related Receptor beta.

      If you need any further assistance in the future, please do not hesitate to contact me.

      Read More

      Abcam Scientific Support

      Answered on Mar 23 2012

      Question

      I have a few specific questions regarding one of your antibodies we used, antibody ab19331, directed against estrogen related receptor beta. We used the ab in immunohistochemistry and found reasonable levels of background, besides clear specific staining. We incorporated these data in a manuscript that now has been reviewed for publication and one of the referees requested antibody validation. Therefore: 1) Could you provide us with detailed information, including images, of the validation experiments you performed? 2) Is it possible to obtain some of the synthetic peptide used to raise the antibody (peptide: DYELSQRHEEPRR), in order for us to block the antibody with peptide prior to immunohistochemistry? Please contact us soon, since we are on short time-scale regarding the resubmission.

      Read More

      Abcam community

      Verified customer

      Asked on Aug 22 2007

      Answer

      Thank you for your enquiry regarding our Estrogen Related Receptor beta antibody (ab19331) I can confirm the western blotting procedure used for testing this antibody was as follows: 1) Block membrane with 5 ml of blocking buffer for 1 hour at room temp., gently agitating solution. 2) Incubate primary antibody diluted 1:250 in blocking buffer for 1 hour at room temp. gently agitating solution. Peptide Pre-incubations: Incubate primary antibody in 100 ul of blocking buffer for 15 min. at room temp with peptide prior to incubation with filter. 3) Wash filter 3X with TBST buffer, 8 min. at room temp. each, gently agitating solution. 4) Incubate secondary antibody diluted 1:30,000 in blocking buffer for 1 hour at room temp. gently agitating solution. 5) Wash filter 3X with TBST buffer, 8 min. at room temp. each, gently agitating solution. 6) Visualize by ECL detection and expose film. Good positive controls for this antibody include: Rat kidney, testes and skeletal muscle. Unfortuantely, I am sorry we do not have a blocking peptide available for this particular antibody. I hope this information is helpful. Should you have any further questions, please do not hestiate to contact us again.

      Read More

      Abcam Scientific Support

      Answered on Aug 22 2007

      Question

      BATCH NUMBER 113689 ORDER NUMBER 105233 DESCRIPTION OF THE PROBLEM high background SAMPLE cell extract PRIMARY ANTIBODY 1:250 dilution, 1-2 hour DETECTION METHOD ECL POSITIVE AND NEGATIVE CONTROLS USED non loading as negative control ANTIBODY STORAGE CONDITIONS -20 degree celcius SAMPLE PREPARATION lysis buffer AMOUNT OF PROTEIN LOADED 40ug ELECTROPHORESIS/GEL CONDITIONS 12% reducing gel TRANSFER AND BLOCKING CONDITIONS 5% milk in PBS-T SECONDARY ANTIBODY donkey anti rabbit IgG ECL HRP-conjugated HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 2 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes ADDITIONAL NOTES When antibody came, tubes felt warm. Indication of non-cold temperature transport.

      Read More

      Abcam community

      Verified customer

      Asked on Dec 13 2005

      Answer

      I'm very sorry to hear you are experiencing problems with ab19331, ab12986 and ab12987. I have looked at the shipping times of your order and would like to reassure you that there were no delays in the shipping. It is not uncommon that antibodies arrive at room temperature, especially when they travel some long distances and we have done stability studies in our laboratories and found that activity will not be impaired by such conditions. A high background problem is often due to the blocking agent or the secondary antibody; in our laboratory we block with 5% BSA (1hr) and find this buffer gives us the best results, while milk can give background problems so I think switching to BSA will help you a lot. Have you also tested if the secondary antibody works well with other primaries and if alone(no primary)it gives you some staining? For ab19331 the antibody has been tested in rat kidney samples and therefore I would like to recommend running this positive control as well as doing a nuclear extraction from your samples to concentrate the protein as you may have low levels of protein (you do not mention what cells you are studying), as well as incubating the antibody overnight in TBST. The antibodies ab12986 and ab12987 have not been tested in WB and therefore it is very difficult to know if those antibodies should work in this application and I would recommend trying the same protocol advice given above for ab19331. These antibodies have only been tested in human tissue so a human positive control is essential (e.g testis tissue). I think the above suggestions should help you a lot, if you still experience problems please do not hesitate to contact us. Ab19331 is guaranteed to work in WB in rat samples and if you still experience problems with this antibody in this application and species with the above changes I can offer you a replacement or refund,

      Read More

      Abcam Scientific Support

      Answered on Dec 13 2005

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2023 Abcam plc. All rights reserved.