Anti-Estrogen Related Receptor beta antibody (ab19331)
- Datasheet
- Specific References (4)
- Protocols
Overview
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Product nameAnti-Estrogen Related Receptor beta antibody
See all Estrogen Related Receptor beta primary antibodies -
DescriptionRabbit polyclonal to Estrogen Related Receptor beta
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-Fr, WB, IP, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rat
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Immunogen
Synthetic peptide:
DYELSQRHEEPRR
, corresponding to amino acids 376-388 of Mouse Estrogen Related Receptor beta. -
Positive control
- rat kidney lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab19331 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. PubMed: 18179891 | |
| WB | Use a concentration of 2 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). | |
| IP | Use at an assay dependent concentration. | |
| ICC/IF | 1/50 - 1/200. |
Target
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FunctionNuclear receptor, may regulate ESR1 transcriptional activity.
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Involvement in diseaseDefects in ESRRB are the cause of deafness autosomal recessive type 35 (DFNB35) [MIM:608565]. DFNB35 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
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Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 26380 Mouse
- Entrez Gene: 299210 Rat
- SwissProt: Q61539 Mouse
- SwissProt: P11475 Rat
- Unigene: 235550 Mouse
- Unigene: 104984 Rat
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Alternative names
- Err 2 antibody
- ERR b antibody
- ERR B2 antibody
see all
Images
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Western blot - Anti-Estrogen Related Receptor beta antibody (ab19331)Western blot detection of Estrogen Related Receptor beta from 100 ug of rat kidney lysate (1:500).
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Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Related Receptor beta antibody (ab19331)This image is courtesy of an anonymous abreviewer.ab19331 staining Estrogen Related Receptor Beta protein on mouse embryonic stem cell by ICC/IF. The cells were fixed in 1:1 methanol:acetone and blocked in 0.1% BSA plus 1% serum, for 30 minutes at 21°C. The primary antibody was diluted 1/100 and incubated with the cells for 2 hours at 21°C. An Alexa Fluor® 488 conjugated goat anti-rabbit IgG, diluted 1/200, was used as the secondary antibody.
Protocols
References
This product has been referenced in:
- Pacwa A et al. Interplay between estrogen-related receptors and steroidogenesis-controlling molecules in adrenals. In vivo and in vitro study. Acta Histochem 120:456-467 (2018). WB ; Rat . Read more (PubMed: 29778238) »
- Chaudhary S et al. Overexpression of caspase 7 is ERa dependent to affect proliferation and cell growth in breast cancer cells by targeting p21(Cip). Oncogenesis 5:e219 (2016). ChIP . Read more (PubMed: 27089142) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"