Key features and details
- Rabbit polyclonal to Estrogen Sulfotransferase
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Estrogen Sulfotransferase antibody
See all Estrogen Sulfotransferase primary antibodies
DescriptionRabbit polyclonal to Estrogen Sulfotransferase
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
- WB: RT4 cell lysate. IHC-P: Human duodenum tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224761 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 35 kDa.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMay control the level of the estrogen receptor by sulfurylating free estradiol. Maximally sulfates beta-estradiol and estrone at concentrations of 20 nM. Also sulfates dehydroepiandrosterone, pregnenolone, ethinylestradiol, equalenin, diethylstilbesterol and 1-naphthol, at significantly higher concentrations; however, cortisol, testosterone and dopamine are not sulfated.
Tissue specificityLiver, intestine and at lower level in the kidney.
Sequence similaritiesBelongs to the sulfotransferase 1 family.
- Information by UniProt
- EST antibody
- EST-1 antibody
- EST1 antibody
Paraffin-embedded human duodenum tissue stained for Estrogen Sulfotransferase using ab224761 at 1/50 dilution in immunohistochemical analysis.
Anti-Estrogen Sulfotransferase antibody (ab224761) at 1/100 dilution + RT4 (human urinary bladder cancer cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 35 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224761 has not yet been referenced specifically in any publications.