Recombinant Anti-Estrone antibody [EPPTX-R7-94-2] - BSA and Azide free (ab243564)
![Competitive ELISA - Anti-Estrone antibody [EPPTX-R7-94-2] - BSA and Azide free (ab243564)](https://www.abcam.com/ps/products/243/ab243564/Images/ab243564-1-241064ELISA1.jpg "Competitive ELISA - Anti-Estrone antibody [EPPTX-R7-94-2] - BSA and Azide free (ab243564)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPPTX-R7-94-2] to Estrone - BSA and Azide free
- Suitable for: Competitive ELISA
- Reacts with: Human
Overview
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Product name
Anti-Estrone antibody [EPPTX-R7-94-2] - BSA and Azide free
See all Estrone primary antibodies -
Description
Rabbit monoclonal [EPPTX-R7-94-2] to Estrone - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Competitive ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Chemical/ Small Molecule corresponding to Estrone. (Estrone-6-CMO-BSA).
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General notes
ab243564 is the carrier-free version of ab241064. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab243564 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPPTX-R7-94-2 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
Applications
Our Abpromise guarantee covers the use of ab243564 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Competitive ELISA | Use at an assay dependent concentration. |
Target
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Relevance
Estrone, estradiol, and estriol are all estrogens natural to the human body. They belong to the group of steroid hormones, and are mostly responsible for the growth of female characteristics in puberty and also regulation of the menstrual cycle. They are produced primarily in the ovaries but are also manufactured from androstenedione in fat cells, muscle cells, and skin. Oral estrogen is converted to estrone(less potent) by the gut and liver, reversing the normal premenopausal 2:1 ratio of 17b-oestradiol to estrone. Estrone Levels 1. Men 2.90 - 7.50 pg/0.1 ml 2. Women (days of menstrual cycle) 1-10 days 4.30 - 18.0 pg/0.1 ml 11-20 days 7.50 - 19.6 pg/0.1 ml 21-30 days 13.1 - 20.1 pg/0.1 ml -
Cellular localization
Secreted -
Alternative names
- Oestrone antibody
Images
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Competitive ELISA - Anti-Estrone antibody [EPPTX-R7-94-2] - BSA and Azide free (ab243564)
Goat anti-rabbit IgG was coated onto 96-wells. Serial dilution of Estrone-6 (0, 15, 30, 100, 250, 500, 1000, 2000pg/mL) was added (25μL). This was followed by adding of 50μL of HRP-conjugated Estrone and 2.0-2.5ng/mL ab241064 (50μL) into each well. Plates were incubated for 2 hours at room temperature by shaking. After washing, 125μL of Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes without shaking for color development. Then 125μL of stopping solution (acid solution) was dispensed and OD was read at 450nm on a microplate reader within 5 minutes.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241064). -
![Competitive ELISA - Anti-Estrone antibody [EPPTX-R7-94-2] - BSA and Azide free (ab243564)](https://www.abcam.com/ps/products/243/ab243564/Images/ab243564-2-241064ELISA2.jpg)
Competitive ELISA - Anti-Estrone antibody [EPPTX-R7-94-2] - BSA and Azide free (ab243564)ELISA - Anti-Estrone antibody [EPPTX-R7-94-2] (ab241064) at 2.0 ng/ml.
ab241064 is specific to Estrone-6 and the percent cross reactivity with other compounds is demonstrated in the table.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241064). -
![Anti-Estrone antibody [EPPTX-R7-94-2] - BSA and Azide free (ab243564)](https://www.abcam.com/ps/products/243/ab243564/Images/ab243564-5-benefits-of-recombinant-antibodies.png)
Anti-Estrone antibody [EPPTX-R7-94-2] - BSA and Azide free (ab243564)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab243564 has not yet been referenced specifically in any publications.
