Recombinant
RabMAb

Recombinant Anti-Estrone antibody [EPPTX-R7-94-2] - BSA and Azide free (ab243564)

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Overview

  • Product name

    Anti-Estrone antibody [EPPTX-R7-94-2] - BSA and Azide free
    See all Estrone primary antibodies

  • Description

    Rabbit monoclonal [EPPTX-R7-94-2] to Estrone - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Competitive ELISAmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Chemical/ Small Molecule corresponding to Estrone. (Estrone-6-CMO-BSA).

  • General notes

    ab243564 is the carrier-free version of ab241064. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab243564 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPPTX-R7-94-2

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab243564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Target

  • Relevance

    Estrone, estradiol, and estriol are all estrogens natural to the human body. They belong to the group of steroid hormones, and are mostly responsible for the growth of female characteristics in puberty and also regulation of the menstrual cycle. They are produced primarily in the ovaries but are also manufactured from androstenedione in fat cells, muscle cells, and skin. Oral estrogen is converted to estrone(less potent) by the gut and liver, reversing the normal premenopausal 2:1 ratio of 17b-oestradiol to estrone. Estrone Levels 1. Men 2.90 - 7.50 pg/0.1 ml 2. Women (days of menstrual cycle) 1-10 days 4.30 - 18.0 pg/0.1 ml 11-20 days 7.50 - 19.6 pg/0.1 ml 21-30 days 13.1 - 20.1 pg/0.1 ml

  • Cellular localization

    Secreted

  • Alternative names

    • Oestrone antibody

Images

  • Competitive ELISA - Anti-Estrone antibody [EPPTX-R7-94-2] - BSA and Azide free (ab243564)
    Competitive ELISA - Anti-Estrone antibody [EPPTX-R7-94-2] - BSA and Azide free (ab243564)

    Goat anti-rabbit IgG was coated onto 96-wells. Serial dilution of Estrone-6 (0, 15, 30, 100, 250, 500, 1000, 2000pg/mL) was added (25μL). This was followed by adding of 50μL of HRP-conjugated Estrone and 2.0-2.5ng/mL ab241064 (50μL) into each well. Plates were incubated for 2 hours at room temperature by shaking. After washing, 125μL of Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes without shaking for color development. Then 125μL of stopping solution (acid solution) was dispensed and OD was read at 450nm on a microplate reader within 5 minutes.

    This data was kindly provided by Pantex Division of Bio-Analysis, Inc.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241064).
  • Competitive ELISA - Anti-Estrone antibody [EPPTX-R7-94-2] - BSA and Azide free (ab243564)
    Competitive ELISA - Anti-Estrone antibody [EPPTX-R7-94-2] - BSA and Azide free (ab243564)

    ELISA - Anti-Estrone antibody [EPPTX-R7-94-2] (ab241064) at 2.0 ng/ml.

    ab241064 is specific to Estrone-6 and the percent cross reactivity with other compounds is demonstrated in the table.

    This data was kindly provided by Pantex Division of Bio-Analysis, Inc.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241064).

References

ab243564 has not yet been referenced specifically in any publications.

Publishing research using ab243564? Please let us know so that we can cite the reference in this datasheet.

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