Etanercept ELISA Kit (ab237643)
Key features and details
- Sensitivity: 0.1 µg/ml
- Range: 0.1 µg/ml - 3 µg/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Quantitative
- Reacts with: Human
Overview
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Product name
Etanercept ELISA Kit
See all Etanercept kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall < 15% Inter-assay Sample n Mean SD CV% Overall < 15% -
Sample type
Serum, Plasma -
Assay type
Quantitative -
Sensitivity
0.1 µg/ml -
Range
0.1 µg/ml - 3 µg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 85% - 115% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Note: Etanercept ELISA kit ab237643 is intended for Research Use Only, not for use in diagnostic procedures.
This kit is a sandwich based ELISA kit. The color developed is proportional to the amount of Etanercept in the sample or standard. Results of samples can be determined directly using the standard curve.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called E4374 BioSim™ Etanercept (Human) ELISA Kit. E4374-100 is the same size as the 96 test size of ab237643.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests Assay Buffer 1 x 50ml Etanercept Standard S1 1 x 300µl Etanercept Standard S2 1 x 300µl Etanercept Standard S3 1 x 300µl Etanercept Standard S4 1 x 300µl Etanercept Standard S5 1 x 300µl Etanercept Standard S6 1 x 300µl Etanercept Standard S7 1 x 300µl HRP-conjugate Probe 1 x 12ml Micro ELISA Plate 1 unit Plate sealers 2 units Stop Solution 1 x 12ml TMB substrate 1 x 12ml Wash buffer (20X) 1 x 50ml -
Relevance
Etanercept is a therapeutic fusion protein specific for Tumor Necrosis Factor-Alpha (TNF-a) and is used to treat rheumatic arthritis, intestinal disorders, dermatological diseases and cancer. Etanercept specifically binds to TNF alpha and blocks its interaction with cell surface TNF receptors and reduces the inflammation and subsequently improves the patient’s health. Drug level quantification can be important to adapt patient prescription or to switch to an alternative TNF inhibitor drug.
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab237643 has been referenced in 1 publication.
- Sun G et al. Molybdenum disulfide nanoflowers mediated anti-inflammation macrophage modulation for spinal cord injury treatment. J Colloid Interface Sci 549:50-62 (2019). PubMed: 31015056