Overview

  • Product name

    Etanercept ELISA Kit (Enbrel®)
    See all Etanercept kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 15%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 15%
  • Sample type

    Serum, Plasma
  • Assay type

    Quantitative
  • Sensitivity

    0.1 µg/ml
  • Range

    0.1 µg/ml - 3 µg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 85% - 115%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Etanercept ELISA Kit (Enbrel®) (ab237643) is a highly specific and sensitive kit designed for the in vitro determination of Etanercept in biological matrices such as human serum and plasma. The density of color is proportional to the amount of Etanercept captured from the samples and can be quantified when compared with standard curve. 


    Etanercept (Enbrel®) is a therapeutic fusion protein specific for Tumor Necrosis Factor-Alpha (TNF-α) and is used to treat rheumatic arthritis, intestinal disorders, dermatological diseases and cancer. Etanercept specifically binds to TNF alpha and blocks its interaction with cell surface TNF receptors and reduces the inflammation and subsequently improves the patient’s health. Drug level quantification can be important to adapt patient prescription or to switch to an alternative TNF inhibitor drug.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 96 tests
    Assay Buffer 1 x 50ml
    Etanercept Standard S1 1 x 300µl
    Etanercept Standard S2 1 x 300µl
    Etanercept Standard S3 1 x 300µl
    Etanercept Standard S4 1 x 300µl
    Etanercept Standard S5 1 x 300µl
    Etanercept Standard S6 1 x 300µl
    Etanercept Standard S7 1 x 300µl
    HRP-conjugate Probe 1 x 12ml
    Micro ELISA Plate 1 unit
    Plate sealers 2 units
    Stop Solution 1 x 12ml
    TMB substrate 1 x 12ml
    Wash buffer (20X) 1 x 50ml
  • Relevance

    Etanercept (Enbrel®) is a therapeutic fusion protein specific for Tumor Necrosis Factor-Alpha (TNF-a) and is used to treat rheumatic arthritis, intestinal disorders, dermatological diseases and cancer. Etanercept specifically binds to TNF alpha and blocks its interaction with cell surface TNF receptors and reduces the inflammation and subsequently improves the patient’s health. Drug level quantification can be important to adapt patient prescription or to switch to an alternative TNF inhibitor drug.

Applications

Our Abpromise guarantee covers the use of ab237643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Typical Standard Curve: This standard curve is for demonstration only. A standard curve must be run with each assay.

Protocols

References

ab237643 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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