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Rabbit polyclonal to ETEA
Predicted to work with:
Mouse, Rat, Cow
Recombinant fragment corresponding to a region within amino acids 176-393 of Human ETEA (UniProt ID: Q96CS3).
293T, A431, Jurkat and Raji whole cell lysate; A431 cells; Hs578T xenograft tissue
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 53 kDa.
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/100 - 1/1000.
May play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome.
Broadly expressed, with highest levels in brain.
Contains 1 UBA domain.
Contains 1 UBX domain.
Cytoplasm. Lipid droplet. Endoplasmic reticulum.
Information by UniProt
expressed in T cells and eosinophils in atopic dermatitis antibody
Western blot - Anti-ETEA antibody (ab154064)
Anti-ETEA antibody (ab154064) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 53 kDa 10% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-ETEA antibody (ab154064)
Immunofluorescence analysis of methanol fixed A431 cells, labeling ETEA using ab154064 at 1/200 dilution. Lower image costained with Hoerchst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETEA antibody (ab154064)
Immunohistochemical analysis of paraffin embedded Hs578T xenograft tissue, labeling ETEA using ab154064 at 1/100 dilution.
This product has been referenced in:
et al. Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication. PLoS Pathog 13:e1006674 (2017).
Read more (PubMed: 28985237) »
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"