Product nameAnti-ETFA antibody [2B11AE8]
See all ETFA primary antibodies
DescriptionMouse monoclonal [2B11AE8] to ETFA
Tested applicationsSuitable for: IP, WB, ICC/IF, In-Cell ELISA, Flow Cyt, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length protein. This information is considered to be commercially sensitive.
- HeLa cells Human heart tissue HepG2 whole cells Human liver mitochondria Bovine heart mitochondria Rat liver mitochondria Mouse liver mitochondria
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: HEPES buffered saline
Concentration information loading...
Purification notesThe antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity >95% by SDS-PAGE.
Light chain typekappa
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab110316 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa.|
|ICC/IF||Use a concentration of 4 µg/ml.|
|In-Cell ELISA||Use a concentration of 4 µg/ml.|
|Flow Cyt||Use a concentration of 1 µg/ml.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|IHC-P||Use a concentration of 5 µg/ml.|
FunctionThe electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).
Involvement in diseaseGlutaric aciduria 2A
Sequence similaritiesBelongs to the ETF alpha-subunit/FixB family.
DomainDomain I shares an identical polypeptide fold with the beta subunit ETFB though there is no sequence similarity.
modificationsThe N-terminus is blocked.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- Alpha ETF antibody
- Alpha-ETF antibody
- Electron transfer flavoprotein alpha polypeptide antibody
All lanes : Anti-ETFA antibody [2B11AE8] (ab110316) at 1 µg/ml
Lane 1 : Human heart tissue
Lane 2 : HepG2 whole cells
Lane 3 : Human liver mitochondria
Lane 4 : Bovine heart mitochondria
Lane 5 : Rat liver mitochondria
Lane 6 : Mouse liver mitochondria
Lysates/proteins at 10 µg per lane.
Predicted band size: 35 kDa
Immunocytochemistry image of stained HeLa cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with the antibody (ab110316, 4 µg/mL) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondrial matrix
HL-60 cells were stained with 1 µg/mL ab110316 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
IHC image of ETFA staining in Human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110316, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This product has been referenced in:
- Fisher-Wellman KH et al. Respiratory Phenomics across Multiple Models of Protein Hyperacylation in Cardiac Mitochondria Reveals a Marginal Impact on Bioenergetics. Cell Rep 26:1557-1572.e8 (2019). Read more (PubMed: 30726738) »
- Stauch KL et al. Loss of Pink1 modulates synaptic mitochondrial bioenergetics in the rat striatum prior to motor symptoms: concomitant complex I respiratory defects and increased complex II-mediated respiration. Proteomics Clin Appl 10:1205-1217 (2016). Read more (PubMed: 27568932) »