1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/100 - 1/500.
The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).
Involvement in disease
Glutaric aciduria 2A
Belongs to the ETF alpha-subunit/FixB family.
Domain I shares an identical polypeptide fold with the beta subunit ETFB though there is no sequence similarity.
Electron transfer flavoprotein alpha polypeptide antibody
Electron transfer flavoprotein alpha subunit antibody
Electron transfer flavoprotein subunit alpha mitochondrial antibody
Electron transfer flavoprotein subunit alpha, mitochondrial antibody
Electron transferring flavoprotein alpha polypeptide antibody
Glutaric aciduria II antibody
Western blot - Anti-ETFA antibody (ab153722)
All lanes : Anti-ETFA antibody (ab153722) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract