Overview

  • Product name
  • Description
    Rabbit polyclonal to ETFA
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 87-329 of Human ETFA (Uniprot ID: P13804).

  • Positive control
    • WB: A431, HeLa, HEK-293T, HepG2, C8D30 and PC12 whole cell lysates; ICC/IF: HeLa cells. IHC-P: Human MCF7 xenograft tissue; Mouse brain stem, kidney, urinary bladder and cervix tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab153722 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 35 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/100 - 1/500.

Target

  • Function
    The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).
  • Involvement in disease
    Glutaric aciduria 2A
  • Sequence similarities
    Belongs to the ETF alpha-subunit/FixB family.
  • Domain
    Domain I shares an identical polypeptide fold with the beta subunit ETFB though there is no sequence similarity.
  • Post-translational
    modifications
    The N-terminus is blocked.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha ETF antibody
    • Alpha-ETF antibody
    • Electron transfer flavoprotein alpha polypeptide antibody
    • Electron transfer flavoprotein alpha subunit antibody
    • Electron transfer flavoprotein subunit alpha mitochondrial antibody
    • Electron transfer flavoprotein subunit alpha, mitochondrial antibody
    • Electron transferring flavoprotein alpha polypeptide antibody
    • EMA antibody
    • ETFA antibody
    • ETFA_HUMAN antibody
    • GA2 antibody
    • Glutaric aciduria II antibody
    see all

Images

  • All lanes : Anti-ETFA antibody (ab153722) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 35 kDa

  • ETFA was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract with 5 µg ab153722. Western blot was performed from the immunoprecipitate using ab153722.

    Lane 1: IgG instead of ab153722 in HepG2 whole cell lysate. 

    Lane 2: ab153722 IP in HepG2 whole cell lysate.

  • Paraffin-embedded mouse brain stem tissue stained for ETFA using ab153722 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded mouse kidney tissue stained for ETFA using ab153722 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded mouse urinary bladder tissue stained for ETFA using ab153722 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded mouse cervix tissue stained for ETFA using ab153722 at 1/500 dilution in immunohistochemical analysis.

  • Anti-ETFA antibody (ab153722) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 35 kDa



    10% SDS PAGE
  • Anti-ETFA antibody (ab153722) + C8D30 whole cell lysate at 30 µg

    Predicted band size: 35 kDa



    10 % SDS-PAGE
  • Anti-ETFA antibody (ab153722) at 1/1000 dilution + PC12 whole cell lysate at 30 µg

    Predicted band size: 35 kDa



    10 % SDS-PAGE
  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling ETFA with ab153722 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded Human MCF7 xenograft tissue labeling ETFA with ab153722 at 1/250 dilution.

References

ab153722 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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