Anti-ETFB antibody (ab240593)
Key features and details
- Rabbit polyclonal to ETFB
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-ETFB antibody
See all ETFB primary antibodies -
Description
Rabbit polyclonal to ETFB -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow, Orangutan
-
Immunogen
Synthetic peptide within Human ETFB aa 205-255. The exact sequence is proprietary.
Database link: P38117 -
Positive control
- WB: HeLa, HEK-293T, Jurkat, TMCK-1 and NIH/3T3 whole cell lysate. IP: HEK-293T whole cell lysate.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab240593 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
-
Function
The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). -
Tissue specificity
Abundant in liver, heart and skeletal muscle. A weak expression is seen in the brain, placenta, lung, kidney and pancreas. -
Involvement in disease
Glutaric aciduria 2B -
Sequence similarities
Belongs to the ETF beta-subunit/FixA family. -
Domain
The recognition loop recognizes a hydrophobic patch at the surface of interacting dehydrogenases and acts as a static anchor at the interface. -
Post-translational
modificationsMethylated. Trimethylation at Lys-200 and Lys-203 may negatively regulate the activity in electron transfer from Acyl-CoA dehydrogenases. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
-
Database links
- Entrez Gene: 617210 Cow
- Entrez Gene: 2109 Human
- Entrez Gene: 110826 Mouse
- Entrez Gene: 100171463 Orangutan
- Omim: 130410 Human
- SwissProt: Q2TBV3 Cow
- SwissProt: P38117 Human
- SwissProt: Q9DCW4 Mouse
see all -
Alternative names
- Beta ETF antibody
- Beta-ETF antibody
- Electron transfer flavoprotein beta polypeptide antibody
see all
Images
-
Immunoprecipitation - Anti-ETFB antibody (ab240593)
ETFB was immunoprecipitated from 1 mg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate prepared using NETN lysis buffer with ab240593 at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab240593 at 0.4 μg/mL.
Lane 1: ab240593 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG (-).Detection: Chemiluminescence with an exposure time of 10 seconds.
-
Western blot - Anti-ETFB antibody (ab240593)All lanes : Anti-ETFB antibody (ab240593) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.Detection: Chemiluminescence with an exposure time of 10 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab240593 has not yet been referenced specifically in any publications.
