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    etfb-antibody-ab240593.pdf

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Signal Transduction Metabolism Energy Metabolism
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Anti-ETFB antibody (ab240593)

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Immunoprecipitation - Anti-ETFB antibody (ab240593)
  • Western blot - Anti-ETFB antibody (ab240593)

Key features and details

  • Rabbit polyclonal to ETFB
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human ETFB protein (ab107152)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-ETFB antibody
    See all ETFB primary antibodies
  • Description

    Rabbit polyclonal to ETFB
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Cow, Orangutan
  • Immunogen

    Synthetic peptide within Human ETFB aa 205-255. The exact sequence is proprietary.
    Database link: P38117

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat, TMCK-1 and NIH/3T3 whole cell lysate. IP: HEK-293T whole cell lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Oxidative phosphorylation
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • NIH 3T3 whole cell lysate (ab7179)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human ETFB protein (ab107152)

Applications

Our Abpromise guarantee covers the use of ab240593 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).
  • Tissue specificity

    Abundant in liver, heart and skeletal muscle. A weak expression is seen in the brain, placenta, lung, kidney and pancreas.
  • Involvement in disease

    Glutaric aciduria 2B
  • Sequence similarities

    Belongs to the ETF beta-subunit/FixA family.
  • Domain

    The recognition loop recognizes a hydrophobic patch at the surface of interacting dehydrogenases and acts as a static anchor at the interface.
  • Post-translational
    modifications

    Methylated. Trimethylation at Lys-200 and Lys-203 may negatively regulate the activity in electron transfer from Acyl-CoA dehydrogenases.
  • Cellular localization

    Mitochondrion matrix.
  • Target information above from: UniProt accession P38117 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 617210 Cow
    • Entrez Gene: 2109 Human
    • Entrez Gene: 110826 Mouse
    • Entrez Gene: 100171463 Orangutan
    • Omim: 130410 Human
    • SwissProt: Q2TBV3 Cow
    • SwissProt: P38117 Human
    • SwissProt: Q9DCW4 Mouse
    • SwissProt: Q5RFK0 Orangutan
    • Unigene: 348531 Human
    • Unigene: 30200 Mouse
    see all
  • Alternative names

    • Beta ETF antibody
    • Beta-ETF antibody
    • Electron transfer flavoprotein beta polypeptide antibody
    • Electron transfer flavoprotein beta subunit antibody
    • Electron transfer flavoprotein subunit beta antibody
    • Electron transferring flavoprotein beta polypeptide antibody
    • etfB antibody
    • ETFB_HUMAN antibody
    • FP585 antibody
    • MADD antibody
    see all

Images

  • Immunoprecipitation - Anti-ETFB antibody (ab240593)
    Immunoprecipitation - Anti-ETFB antibody (ab240593)

    ETFB was immunoprecipitated from 1 mg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate prepared using NETN lysis buffer with ab240593 at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab240593 at 0.4 μg/mL.

    Lane 1: ab240593 IP in HEK-293T whole cell lysate.
    Lane 2: Control IgG (-).

    Detection: Chemiluminescence with an exposure time of 10 seconds.

     

     

  • Western blot - Anti-ETFB antibody (ab240593)
    Western blot - Anti-ETFB antibody (ab240593)
    All lanes : Anti-ETFB antibody (ab240593) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 50 µg per lane.


    Detection: Chemiluminescence with an exposure time of 10 seconds.

     

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab240593? Please let us know so that we can cite the reference in this datasheet.

    ab240593 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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