Anti-ETFB antibody (ab240593)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-ETFB antibody
See all ETFB primary antibodies -
Description
Rabbit polyclonal to ETFB -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow, Orangutan
-
Immunogen
Synthetic peptide within Human ETFB aa 205-255. The exact sequence is proprietary.
Database link: P38117 -
Positive control
- WB: HeLa, HEK-293T, Jurkat, TMCK-1 and NIH/3T3 whole cell lysate. IP: HEK-293T whole cell lysate.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab240593 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
-
Function
The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). -
Tissue specificity
Abundant in liver, heart and skeletal muscle. A weak expression is seen in the brain, placenta, lung, kidney and pancreas. -
Involvement in disease
Glutaric aciduria 2B -
Sequence similarities
Belongs to the ETF beta-subunit/FixA family. -
Domain
The recognition loop recognizes a hydrophobic patch at the surface of interacting dehydrogenases and acts as a static anchor at the interface. -
Post-translational
modificationsMethylated. Trimethylation at Lys-200 and Lys-203 may negatively regulate the activity in electron transfer from Acyl-CoA dehydrogenases. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
-
Database links
- Entrez Gene: 617210 Cow
- Entrez Gene: 2109 Human
- Entrez Gene: 110826 Mouse
- Entrez Gene: 100171463 Orangutan
- Omim: 130410 Human
- SwissProt: Q2TBV3 Cow
- SwissProt: P38117 Human
- SwissProt: Q9DCW4 Mouse
see all -
Alternative names
- Beta ETF antibody
- Beta-ETF antibody
- Electron transfer flavoprotein beta polypeptide antibody
see all
Images
-
Immunoprecipitation - Anti-ETFB antibody (ab240593)ETFB was immunoprecipitated from 1 mg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate prepared using NETN lysis buffer with ab240593 at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab240593 at 0.4 μg/mL.
Lane 1: ab240593 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG (-).Detection: Chemiluminescence with an exposure time of 10 seconds.
-
Western blot - Anti-ETFB antibody (ab240593)All lanes : Anti-ETFB antibody (ab240593) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.Detection: Chemiluminescence with an exposure time of 10 seconds.
Datasheets and documents
References
ab240593 has not yet been referenced specifically in any publications.
