Key features and details
- Goat polyclonal to ETFB - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ETFB antibody - C-terminal
See all ETFB primary antibodies
DescriptionGoat polyclonal to ETFB - C-terminal
Specificityab219638 is expected to recognize both reported isoforms (NP_001976.1; NP_001014763.1).
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig, Orangutan
- Mouse liver lysate; Human liver tissue.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab219638 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 28 kDa).|
|IHC-P||Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionThe electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).
Tissue specificityAbundant in liver, heart and skeletal muscle. A weak expression is seen in the brain, placenta, lung, kidney and pancreas.
Involvement in diseaseGlutaric aciduria 2B
Sequence similaritiesBelongs to the ETF beta-subunit/FixA family.
DomainThe recognition loop recognizes a hydrophobic patch at the surface of interacting dehydrogenases and acts as a static anchor at the interface.
modificationsMethylated. Trimethylation at Lys-200 and Lys-203 may negatively regulate the activity in electron transfer from Acyl-CoA dehydrogenases.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- Beta ETF antibody
- Beta-ETF antibody
- Electron transfer flavoprotein beta polypeptide antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human liver tissue labeling ETFB with ab219638 at 3.75 µg/ml.
Anti-ETFB antibody - C-terminal (ab219638) at 0.01 µg/ml + Mouse liver lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 28 kDa
Primary antibody incubation was 1 hour.
ab219638 has not yet been referenced specifically in any publications.