Overview

  • Product name

    Anti-ETFB antibody - C-terminal
    See all ETFB primary antibodies
  • Description

    Goat polyclonal to ETFB - C-terminal
  • Host species

    Goat
  • Specificity

    ab219638 is expected to recognize both reported isoforms (NP_001976.1; NP_001014763.1).
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human ETFB aa 231-243 (C terminal) (Cysteine residue). (NP_001976.1; NP_001014763.1).
    Sequence:

    PQRTAGVKVETTE


    Database link: P38117

  • Positive control

    • Mouse liver lysate; Human liver tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab219638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 28 kDa).
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).
  • Tissue specificity

    Abundant in liver, heart and skeletal muscle. A weak expression is seen in the brain, placenta, lung, kidney and pancreas.
  • Involvement in disease

    Glutaric aciduria 2B
  • Sequence similarities

    Belongs to the ETF beta-subunit/FixA family.
  • Domain

    The recognition loop recognizes a hydrophobic patch at the surface of interacting dehydrogenases and acts as a static anchor at the interface.
  • Post-translational
    modifications

    Methylated. Trimethylation at Lys-200 and Lys-203 may negatively regulate the activity in electron transfer from Acyl-CoA dehydrogenases.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • Beta ETF antibody
    • Beta-ETF antibody
    • Electron transfer flavoprotein beta polypeptide antibody
    • Electron transfer flavoprotein beta subunit antibody
    • Electron transfer flavoprotein subunit beta antibody
    • Electron transferring flavoprotein beta polypeptide antibody
    • etfB antibody
    • ETFB_HUMAN antibody
    • FP585 antibody
    • MADD antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human liver tissue labeling ETFB with ab219638 at 3.75 µg/ml.

  • Anti-ETFB antibody - C-terminal (ab219638) at 0.01 µg/ml + Mouse liver lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 28 kDa



    Primary antibody incubation was 1 hour.

References

ab219638 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab219638.
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