Product nameAnti-ETFDH antibody [3H2BG1]
See all ETFDH primary antibodies
DescriptionMouse monoclonal [3H2BG1] to ETFDH
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Cow, Human
The details of the immunogen for this antibody are not available.
- Human cell, heart and liver lysates, Rat liver lysates, Mouse liver lysates, Bovine heart mitochondria lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal heart muscle.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline
Concentration information loading...
PurityAmmonium Sulphate Precipitation
Purification notesProduced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation. Near homogeneity as judged by SDS-PAGE.
Light chain typekappa
Our Abpromise guarantee covers the use of ab126576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa.|
|IHC-P||Use a concentration of 5 µg/ml.|
FunctionAccepts electrons from ETF and reduces ubiquinone.
Involvement in diseaseDefects in ETFDH are the cause of glutaric aciduria type 2C (GA2C) [MIM:231680]. GA2C is an autosomal recessively inherited disorder of fatty acid, amino acid, and choline metabolism. It is characterized by multiple acyl-CoA dehydrogenase deficiencies resulting in large excretion not only of glutaric acid, but also of lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids.
Sequence similaritiesBelongs to the ETF-QO/fixC family.
Contains 1 4Fe-4S ferredoxin-type domain.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- Electron transfer flavoprotein ubiquinone oxidoreductase antibody
- Electron transfer flavoprotein-ubiquinone oxidoreductase antibody
- electron transferring flavoprotein dehydrogenase antibody
All lanes : Anti-ETFDH antibody [3H2BG1] (ab126576) at 1 µg/ml
Lane 1 : Human heart lysate at 20 µg
Lane 2 : Human liver lysate at 20 µg
Lane 3 : Human cell lysate at 20 µg
Lane 4 : Rat liver lysate at 20 µg
Lane 5 : Mouse liver lysate at 20 µg
Lane 6 : Bovine heart mitochondria
at 10 µg
All lanes : GAM-HRP at 1/3000 dilution
Predicted band size: 68 kDa
The high background signal in Mouse tissue sample was caused by the direct reaction between the Mouse IgG in Mouse tissue preps and the goat anti-Mouse secondary antibody.
IHC image of ETFDH staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab126576, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This product has been referenced in:
- Fisher-Wellman KH et al. Respiratory Phenomics across Multiple Models of Protein Hyperacylation in Cardiac Mitochondria Reveals a Marginal Impact on Bioenergetics. Cell Rep 26:1557-1572.e8 (2019). Read more (PubMed: 30726738) »
- Zhu M et al. Riboflavin-responsive multiple Acyl-CoA dehydrogenation deficiency in 13 cases, and a literature review in mainland Chinese patients. J Hum Genet N/A:N/A (2014). Human . Read more (PubMed: 24522293) »