Product nameAnti-ETFDH antibody - C-terminal
See all ETFDH primary antibodies
DescriptionRabbit polyclonal to ETFDH - C-terminal
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human ETFDH aa 318-617 (C terminal).
LVALGLVVGLDYQNPYLSPFREFQRWKHHPSIRPTLEGGKRIAYGARALN EGGFQSIPKLTFPGGLLIGCSPGFMNVPKIKGTHTAMKSGILAAESIFNQ LTSENLQSKTIGLHVTEYEDNLKNSWVWKELYSVRNIRPSCHGVLGVYGG MIYTGIFYWILRGMEPWTLKHKGSDFERLKPAKDCTPIEYPKPDGQISFD LLSSVALSGTNHEHDQPAHLTLRDDSIPVNRNLSIYDGPEQRFCPAGVYE FVPVEQGDGFRLQINAQNCVHCKTCDIKDPSQNINWVVPEGGGGPAYNGM
Database link: Q16134
- WB: Mouse liver and kidney tissue lysates; HEK-293T whole cell lysate. IHC-P: Human kidney and small intestine tissues. ICC/IF: PC-3 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224663 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Detects a band of approximately 68 kDa (predicted molecular weight: 63,69 kDa).|
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
FunctionAccepts electrons from ETF and reduces ubiquinone.
Involvement in diseaseDefects in ETFDH are the cause of glutaric aciduria type 2C (GA2C) [MIM:231680]. GA2C is an autosomal recessively inherited disorder of fatty acid, amino acid, and choline metabolism. It is characterized by multiple acyl-CoA dehydrogenase deficiencies resulting in large excretion not only of glutaric acid, but also of lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids.
Sequence similaritiesBelongs to the ETF-QO/fixC family.
Contains 1 4Fe-4S ferredoxin-type domain.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- Electron transfer flavoprotein ubiquinone oxidoreductase antibody
- Electron transfer flavoprotein-ubiquinone oxidoreductase antibody
- electron transferring flavoprotein dehydrogenase antibody
All lanes : Anti-ETFDH antibody - C-terminal (ab224663) at 1/500 dilution
Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 63,69 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?
Paraffin-embedded human kidney tissue stained for ETFDH using ab224663 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human small intestine tissue stained for ETFDH using ab224663 at 1/100 dilution in immunohistochemical analysis.
PC-3 (human prostate adenocarcinoma cell line) cells stained for ETFDH (green) using ab224663 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
ab224663 has not yet been referenced specifically in any publications.