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    etfdh-antibody-c-terminal-ab224663.pdf

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Anti-ETFDH antibody - C-terminal (ab224663)

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Western blot - Anti-ETFDH antibody - C-terminal (ab224663)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFDH antibody - C-terminal (ab224663)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFDH antibody - C-terminal (ab224663)
  • Immunocytochemistry/ Immunofluorescence - Anti-ETFDH antibody - C-terminal (ab224663)

Key features and details

  • Rabbit polyclonal to ETFDH - C-terminal
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-ETFDH antibody - C-terminal
    See all ETFDH primary antibodies
  • Description

    Rabbit polyclonal to ETFDH - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human ETFDH aa 318-617 (C terminal).
    Sequence:

    LVALGLVVGLDYQNPYLSPFREFQRWKHHPSIRPTLEGGKRIAYGARALN EGGFQSIPKLTFPGGLLIGCSPGFMNVPKIKGTHTAMKSGILAAESIFNQ LTSENLQSKTIGLHVTEYEDNLKNSWVWKELYSVRNIRPSCHGVLGVYGG MIYTGIFYWILRGMEPWTLKHKGSDFERLKPAKDCTPIEYPKPDGQISFD LLSSVALSGTNHEHDQPAHLTLRDDSIPVNRNLSIYDGPEQRFCPAGVYE FVPVEQGDGFRLQINAQNCVHCKTCDIKDPSQNINWVVPEGGGGPAYNGM


    Database link: Q16134
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Mouse liver and kidney tissue lysates; HEK-293T whole cell lysate. IHC-P: Human kidney and small intestine tissues. ICC/IF: PC-3 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Mouse liver tissue lysate - total protein (ab29301)
    • Mouse kidney normal tissue lysate - total protein (ab29305)

Applications

Our Abpromise guarantee covers the use of ab224663 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Detects a band of approximately 68 kDa (predicted molecular weight: 63,69 kDa).
IHC-P 1/20 - 1/200.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Accepts electrons from ETF and reduces ubiquinone.
  • Involvement in disease

    Defects in ETFDH are the cause of glutaric aciduria type 2C (GA2C) [MIM:231680]. GA2C is an autosomal recessively inherited disorder of fatty acid, amino acid, and choline metabolism. It is characterized by multiple acyl-CoA dehydrogenase deficiencies resulting in large excretion not only of glutaric acid, but also of lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids.
  • Sequence similarities

    Belongs to the ETF-QO/fixC family.
    Contains 1 4Fe-4S ferredoxin-type domain.
  • Cellular localization

    Mitochondrion inner membrane.
  • Target information above from: UniProt accession Q16134 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2110 Human
    • Entrez Gene: 66841 Mouse
    • Omim: 231675 Human
    • SwissProt: Q16134 Human
    • SwissProt: Q921G7 Mouse
    • Unigene: 155729 Human
    • Unigene: 28336 Mouse
    • Alternative names

      • Electron transfer flavoprotein ubiquinone oxidoreductase antibody
      • Electron transfer flavoprotein-ubiquinone oxidoreductase antibody
      • electron transferring flavoprotein dehydrogenase antibody
      • Electron-transferring-flavoprotein dehydrogenase antibody
      • ETF dehydrogenase antibody
      • ETF QO antibody
      • ETF ubiquinone oxidoreductase antibody
      • ETF-QO antibody
      • ETF-ubiquinone oxidoreductase antibody
      • ETFD_HUMAN antibody
      • Etfdh antibody
      • MADD antibody
      • mitochondrial antibody
      see all

    Images

    • Western blot - Anti-ETFDH antibody - C-terminal (ab224663)
      Western blot - Anti-ETFDH antibody - C-terminal (ab224663)
      All lanes : Anti-ETFDH antibody - C-terminal (ab224663) at 1/500 dilution

      Lane 1 : Mouse liver tissue lysate
      Lane 2 : Mouse kidney tissue lysate
      Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution

      Predicted band size: 63,69 kDa
      Observed band size: 68 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFDH antibody - C-terminal (ab224663)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFDH antibody - C-terminal (ab224663)

      Paraffin-embedded human kidney tissue stained for ETFDH using ab224663 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFDH antibody - C-terminal (ab224663)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFDH antibody - C-terminal (ab224663)

      Paraffin-embedded human small intestine tissue stained for ETFDH using ab224663 at 1/100 dilution in immunohistochemical analysis.

    • Immunocytochemistry/ Immunofluorescence - Anti-ETFDH antibody - C-terminal (ab224663)
      Immunocytochemistry/ Immunofluorescence - Anti-ETFDH antibody - C-terminal (ab224663)

      PC-3 (human prostate adenocarcinoma cell line) cells stained for ETFDH (green) using ab224663 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab224663? Please let us know so that we can cite the reference in this datasheet.

    ab224663 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-ETFDH antibody - C-terminal

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Euglena Cell lysate - whole cell (Euglena gracilis whole cell)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    15 µg
    Treatment
    RNAi for EgETFDH
    Specification
    Euglena gracilis whole cell
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    昌美 中澤

    Verified customer

    Submitted May 08 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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