Anti-ETFDH antibody - C-terminal (ab224663)

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Overview

  • Product name

    Anti-ETFDH antibody - C-terminal
    See all ETFDH primary antibodies

  • Description

    Rabbit polyclonal to ETFDH - C-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment corresponding to Human ETFDH aa 318-617 (C terminal).
    Sequence:

    LVALGLVVGLDYQNPYLSPFREFQRWKHHPSIRPTLEGGKRIAYGARALN EGGFQSIPKLTFPGGLLIGCSPGFMNVPKIKGTHTAMKSGILAAESIFNQ LTSENLQSKTIGLHVTEYEDNLKNSWVWKELYSVRNIRPSCHGVLGVYGG MIYTGIFYWILRGMEPWTLKHKGSDFERLKPAKDCTPIEYPKPDGQISFD LLSSVALSGTNHEHDQPAHLTLRDDSIPVNRNLSIYDGPEQRFCPAGVYE FVPVEQGDGFRLQINAQNCVHCKTCDIKDPSQNINWVVPEGGGGPAYNGM


    Database link: Q16134

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Mouse liver and kidney tissue lysates; HEK-293T whole cell lysate. IHC-P: Human kidney and small intestine tissues. ICC/IF: PC-3 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab224663 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Detects a band of approximately 68 kDa (predicted molecular weight: 63,69 kDa).
IHC-P 1/20 - 1/200.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Accepts electrons from ETF and reduces ubiquinone.

  • Involvement in disease

    Defects in ETFDH are the cause of glutaric aciduria type 2C (GA2C) [MIM:231680]. GA2C is an autosomal recessively inherited disorder of fatty acid, amino acid, and choline metabolism. It is characterized by multiple acyl-CoA dehydrogenase deficiencies resulting in large excretion not only of glutaric acid, but also of lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids.

  • Sequence similarities

    Belongs to the ETF-QO/fixC family.
    Contains 1 4Fe-4S ferredoxin-type domain.

  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Electron transfer flavoprotein ubiquinone oxidoreductase antibody
      • Electron transfer flavoprotein-ubiquinone oxidoreductase antibody
      • electron transferring flavoprotein dehydrogenase antibody
      • Electron-transferring-flavoprotein dehydrogenase antibody
      • ETF dehydrogenase antibody
      • ETF QO antibody
      • ETF ubiquinone oxidoreductase antibody
      • ETF-QO antibody
      • ETF-ubiquinone oxidoreductase antibody
      • ETFD_HUMAN antibody
      • Etfdh antibody
      • MADD antibody
      • mitochondrial antibody
      see all

    Images

    • Western blot - Anti-ETFDH antibody - C-terminal (ab224663)
      Western blot - Anti-ETFDH antibody - C-terminal (ab224663)
      All lanes : Anti-ETFDH antibody - C-terminal (ab224663) at 1/500 dilution

      Lane 1 : Mouse liver tissue lysate
      Lane 2 : Mouse kidney tissue lysate
      Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution

      Predicted band size: 63,69 kDa
      Observed band size: 68 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFDH antibody - C-terminal (ab224663)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFDH antibody - C-terminal (ab224663)

      Paraffin-embedded human kidney tissue stained for ETFDH using ab224663 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFDH antibody - C-terminal (ab224663)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFDH antibody - C-terminal (ab224663)

      Paraffin-embedded human small intestine tissue stained for ETFDH using ab224663 at 1/100 dilution in immunohistochemical analysis.

    • Immunocytochemistry/ Immunofluorescence - Anti-ETFDH antibody - C-terminal (ab224663)
      Immunocytochemistry/ Immunofluorescence - Anti-ETFDH antibody - C-terminal (ab224663)

      PC-3 (human prostate adenocarcinoma cell line) cells stained for ETFDH (green) using ab224663 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

    References

    ab224663 has not yet been referenced specifically in any publications.

    Publishing research using ab224663? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Western blot abreview for Anti-ETFDH antibody - C-terminal

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Euglena Cell lysate - whole cell (Euglena gracilis whole cell)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    15 µg
    Treatment
    RNAi for EgETFDH
    Specification
    Euglena gracilis whole cell
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    昌美 中澤

    Verified customer

    Submitted May 08 2018

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