Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 10 nM
Product nameEthanol Assay Kit
See all Ethanol kits
Sample typeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
Sensitivity> 10 nM
Range2 µM - 200 µM
Assay time1h 00m
Ethanol Assay Kit ab65343 provides a simple, rapid, and sensitive method for accurate quantification of ethanol concentration in a variety of biological samples such as serum, plasma, other body fluids, foods, beverages and growth media.
In the ethanol assay protocl, alcohol oxidase oxidizes ethanol to generate H2O2 which reacts with a probe to generate color (absorbance max 570 nm) and fluorescence (Ex/Em 535/587 nm).
The kit detects 0.1-10 ppm alcohol (~10-800 nM).
Ethanol assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min at 37ºC
- analyze with microplate reader
This product is manufactured by BioVision, an Abcam company and was previously called K620 Ethanol Colorimetric/Fluorometric Assay Kit. K620-100 is the same size as the 100 test size of ab65343.
PLEASE NOTE: Extreme care should be taken to ensure that no alcohol vapors (ethanol, methanol, propanol) are in the laboratory air where this assay is to be performed. Alcohol vapors in the air will be rapidly absorbed by kit components resulting in very high background making the kit unusable. Laboratories where HPLC equipment and solvents are standing or where alcohol is used to wipe down laboratory benches or equipment are inappropriate locations to perform this assay.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests 2000 tests Ethanol Assay Buffer WM 1 x 25ml 20 x 25ml Ethanol Enzyme Mix Green 1 vial 20 vials Ethanol Probe (in DMSO, anhydrous) Red 1 x 200µl 20 x 200µl Ethanol Standard Yellow 1 x 500µl 20 x 500µl
RelevanceAlcohol (ethanol C2H5OH) is a clear colorless liquid rapidly absorbed from the gastrointestinal tract and distributed through the body. Ethanol has a depressive effect on the central nervous system and because of its psychoactive effects, it is considered a drug. Ethanol is metabolized by the body as an enerfy-providing carbohydrate nutrient, as it metabolized into acetyl CoA, an intermediate common with glucose than can be used for energy in the citric acid cycle (TCA) or for biosynthesis. Ethanol within the human body is converted into acetaldehyde by alcohol dehydrogenase and then into acetic acid by acetaldehyde dehydriogenase. Acetaldehyde, the product of the first step of the alcohol breakdown, is linked to most of the toxic clinical effects of alcohol.
- Ethyl alcohol
Ethanol in blood plasma was analyzed using ethanol assay kit (ab65343). Ethanol concentrations were determined 60 minutes after urethane administration in P0/1 mice and rats.
0.5 nmol of ethanol was added to diluted human biological fluids (1/10) and spiked samples were measured fluorimetrically (background subtracted, duplicates; +/- SD).
5 nmol of ethanol was added to diluted human biological fluids (1/10) and spiked samples were measured colourimetrically (background subtracted, duplicates; +/- SD).
Standard curves with background signal subtracted (duplicates; +/- SD).
Ethanol Standard Curve. Ethanol standard was diluted as described and the assay was performed according to the kit instructions.
ab65343 has been referenced in 21 publications.
- Sampedro-Piquero P et al. Treadmill Exercise Buffers Behavioral Alterations Related to Ethanol Binge-Drinking in Adolescent Mice. Brain Sci 10:N/A (2020). PubMed: 32825478
- Brunori G et al. NOP Receptor Antagonists Decrease Alcohol Drinking in the Dark in C57BL/6J Mice. Alcohol Clin Exp Res 43:2167-2178 (2019). PubMed: 31386211
- Cananzi SG & Mayhan WG In Utero Exposure to Alcohol Impairs Reactivity of Cerebral Arterioles and Increases Susceptibility of the Brain to Damage Following Ischemia/Reperfusion in Adulthood. Alcohol Clin Exp Res 43:607-616 (2019). PubMed: 30748017
- McCarter KD et al. Influence of low-dose alcohol consumption on post-ischemic inflammation: Role of cystathionine ?-lyase. Alcohol 76:81-89 (2019). PubMed: 30597416
- Xu G et al. Dose-Dependent Influences of Ethanol on Ischemic Stroke: Role of Inflammation. Front Cell Neurosci 13:6 (2019). PubMed: 30853895