Anti-ethanolamine kinase antibody (ab236615)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-ethanolamine kinase antibody -
Description
Rabbit polyclonal to ethanolamine kinase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human ethanolamine kinase aa 90-258.
Sequence:MANYIHVPPGSPEVPKLNVTVQDQEEHRCREGALSLLQHLRPHWDPQEVT LQLFTDGITNKLIGCYVGNTMEDVVLVRIYGNKTELLVDRDEEVKSFRVL QAHGCAPQLYCTFNNGLCYEFIQGEALDPKHVCNPAIFSLSSLTLCKGKT TRCFGLTGCRGSRLLLSFF
Database link: Q9HBU6-2 -
Positive control
- WB: A549, A431, HepG2 and MCF7 whole cell lysate. ICC/IF: A549 cells. IHC-P: Human thyroid tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.03% Proclin
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab236615 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/2000. | |
| IHC-P | 1/20 - 1/200. | |
| ICC/IF | 1/50 - 1/200. |
Target
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Function
Highly specific for ethanolamine phosphorylation. May be a rate-controlling step in phosphatidylethanolamine biosynthesis. -
Tissue specificity
Expressed in kidney, liver, placenta, heart, leukocyte, ovary and testis. -
Pathway
Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 1/3. -
Sequence similarities
Belongs to the choline/ethanolamine kinase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 55500 Human
- Omim: 609858 Human
- SwissProt: Q9HBU6 Human
- Unigene: 29464 Human
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Alternative names
- 1110061E11Rik antibody
- 4930555L11Rik antibody
- AI195356 antibody
see all
Images
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Western blot - Anti-ethanolamine kinase antibody (ab236615)All lanes : Anti-ethanolamine kinase antibody (ab236615) at 1/500 dilution
Lane 1 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 2 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ethanolamine kinase antibody (ab236615)Paraffin-embedded human thyroid tissue stained for ethanolamine kinase with ab236615 at a 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-ethanolamine kinase antibody (ab236615)A549 (Human lung carcinoma cell line) cells stained for ethanolamine kinase (Green) using ab236615 at a 1/100 dilution in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
References
ab236615 has not yet been referenced specifically in any publications.
