Overview

  • Product name
    Anti-ETHE1 antibody [EPR11697]
    See all ETHE1 primary antibodies
  • Description
    Rabbit monoclonal [EPR11697] to ETHE1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ETHE1 aa 200 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: O95571

  • Positive control
    • HepG2, HeLa, SW480 and Jurkat cell lysates; Cervical carcinoma and colon tissues
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab174302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
IHC-P 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function
      Probably plays an important role in metabolic homeostasis in mitochondria. May function as a nuclear-cytoplasmic shuttling protein that binds transcription factor RELA/NFKB3 in the nucleus and exports it to the cytoplasm. Suppresses p53-induced apoptosis by preventing nuclear localization of RELA.
    • Tissue specificity
      Ubiquitously expressed.
    • Involvement in disease
      Defects in ETHE1 are a cause of ethylmalonic encephalopathy (EE) [MIM:602473]. EE is an autosomal recessive disorder characterized by neurodevelopmental delay and regression, recurrent petechiae, acrocyanosis, diarrhea, leading to death in the first decade of life. It is also associated with persistent lactic acidemia and ethylmalonic and methylsuccinic aciduria.
    • Sequence similarities
      Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.
    • Cellular localization
      Cytoplasm. Nucleus. Mitochondrion matrix. According to PubMed:12398897, it is cytoplasmic and nuclear. According to PubMed:14732903, it is found in the mitochondrial matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • Ethe1 antibody
      • ETHE1 protein, mitochondrial precursor antibody
      • ETHE1_HUMAN antibody
      • ethylmalonic encephalopathy 1 antibody
      • Ethylmalonic encephalopathy protein 1 antibody
      • hepatoma subtracted clone one antibody
      • Hepatoma subtracted clone one protein antibody
      • HSCO antibody
      • mitochondrial antibody
      • Protein ETHE1 antibody
      • Protein ETHE1 mitochondrial antibody
      • YF13H12 antibody
      see all

    Images

    • All lanes : Anti-HPS1 antibody [EP8429(2)] (ab174301) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : SW480 cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 28 kDa

    • Immunohistochemical analysis of paraffin-embedded cervical carcinoma tissue labeling ETHE1 with ab174302 at 1/100 dilution.

    • Immunohistochemical analysis of paraffin-embedded colon tissue labeling ETHE1 with ab174302 at 1/100 dilution.

    References

    This product has been referenced in:
    • Zhang S  et al. hsa-miR-29c-3p regulates biological function of colorectal cancer by targeting SPARC. Oncotarget 8:104508-104524 (2017). Read more (PubMed: 29262657) »

    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab174302.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up