Recombinant Anti-ETHE1 antibody [EPR11697] - BSA and Azide free (ab249805)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11697] to ETHE1 - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-ETHE1 antibody [EPR11697] - BSA and Azide free
See all ETHE1 primary antibodies -
Description
Rabbit monoclonal [EPR11697] to ETHE1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
ab249805 is the carrier-free version of ab174302.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR11697 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Conjugation kits
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab249805 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 28 kDa.
|
Notes |
---|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 28 kDa. |
Target
-
Function
Probably plays an important role in metabolic homeostasis in mitochondria. May function as a nuclear-cytoplasmic shuttling protein that binds transcription factor RELA/NFKB3 in the nucleus and exports it to the cytoplasm. Suppresses p53-induced apoptosis by preventing nuclear localization of RELA. -
Tissue specificity
Ubiquitously expressed. -
Involvement in disease
Defects in ETHE1 are a cause of ethylmalonic encephalopathy (EE) [MIM:602473]. EE is an autosomal recessive disorder characterized by neurodevelopmental delay and regression, recurrent petechiae, acrocyanosis, diarrhea, leading to death in the first decade of life. It is also associated with persistent lactic acidemia and ethylmalonic and methylsuccinic aciduria. -
Sequence similarities
Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family. -
Cellular localization
Cytoplasm. Nucleus. Mitochondrion matrix. According to PubMed:12398897, it is cytoplasmic and nuclear. According to PubMed:14732903, it is found in the mitochondrial matrix. - Information by UniProt
-
Database links
- Entrez Gene: 23474 Human
- Entrez Gene: 66071 Mouse
- Entrez Gene: 292710 Rat
- Omim: 608451 Human
- SwissProt: O95571 Human
- SwissProt: Q9DCM0 Mouse
- Unigene: 7486 Human
- Unigene: 29553 Mouse
-
Alternative names
- Ethe1 antibody
- ETHE1 protein, mitochondrial precursor antibody
- ETHE1_HUMAN antibody
see all
Images
-
All lanes : Anti-HPS1 antibody [EP8429(2)] (ab174301) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : SW480 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 28 kDaThis data was developed using ab174302, the same antibody clone in a different buffer formulation.
-
This data was developed using ab174302, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded cervical carcinoma tissue labeling ETHE1 with ab174302 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
This data was developed using ab174302, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded colon tissue labeling ETHE1 with ab174302 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab249805 has not yet been referenced specifically in any publications.