Key features and details
- Rabbit polyclonal to ETS1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ETS1 antibody
See all ETS1 primary antibodies
DescriptionRabbit polyclonal to ETS1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Medaka fish
Synthetic peptide, corresponding to a region within amino acids 175-225 of Human ETS1 (NP_005229.1).
- Jurkat whole cell lysate
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab124282 was affinity purified using an epitope specific to ETS1 immobilized on solid support .
Our Abpromise guarantee covers the use of ab124282 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000.|
|IP||Use at 2-10 µg/mg of lysate.|
Sequence similaritiesBelongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain.
modificationsSumoylated on Lys-15 and Lys-227, preferentially by SUMO2; which inhibits transcriptional activity.
Ubiquitinated; which induces proteasomal degradation.
- Information by UniProt
- Avian erythroblastosis virus E26 (v ets) oncogene homolog 1 antibody
- C ets 1 protein antibody
- c-ets-1 antibody
All lanes : Anti-ETS1 antibody (ab124282) at 0.1 µg/ml
Lane 1 : Jurkat whole cell lysate at 50 µg
Lane 2 : Jurkat whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : HeLa whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 3 minutes
ab124282 at 1 µg/ml staining ETS1 in Jurkat whole cell lysate immunoprecipitated using ab124282 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: control IgG.
Predicted band size : 50 kDa. Exposure time: 30 seconds
ab124282 has been referenced in 3 publications.
- Laporte AN et al. HDAC and Proteasome Inhibitors Synergize to Activate Pro-Apoptotic Factors in Synovial Sarcoma. PLoS One 12:e0169407 (2017). WB ; Human . PubMed: 28056055
- Laporte AN et al. Death by HDAC Inhibition in Synovial Sarcoma Cells. Mol Cancer Ther 16:2656-2667 (2017). PubMed: 28878027
- Pallai R et al. Ets1 and Elk1 transcription factors regulate cancerous inhibitor of protein phosphatase 2A expression in cervical and endometrial carcinoma cells. Transcription 3:323-35 (0). PubMed: 23117818