Product nameAnti-ETS1 antibody
See all ETS1 primary antibodies
DescriptionRabbit polyclonal to ETS1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Dog, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide within Human ETS1 aa 391-441. The exact sequence is proprietary. (NP_005229.1).
Database link: P14921
- WB: Jurkat whole cell lysate. IP: Jurkat whole cell lysate.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab225868 in the following tested applications.
|WB||1/2000 - 1/10000. Predicted molecular weight: 50 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
Sequence similaritiesBelongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain.
modificationsSumoylated on Lys-15 and Lys-227, preferentially by SUMO2; which inhibits transcriptional activity.
Ubiquitinated; which induces proteasomal degradation.
- Information by UniProt
- Avian erythroblastosis virus E26 (v ets) oncogene homolog 1 antibody
- C ets 1 protein antibody
- c-ets-1 antibody
All lanes : Anti-ETS1 antibody (ab225868) at 0.1 µg/ml
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 2 : Jurkat whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 50 kDa
Exposure time: 3 minutes
ETS1 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225868 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225868 at 1 µg/ml.
Lane 1: ab225868 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
ab225868 has not yet been referenced specifically in any publications.