Key features and details
- Rabbit polyclonal to ETS1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ETS1 antibody
See all ETS1 primary antibodies
DescriptionRabbit polyclonal to ETS1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Dog, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide within Human ETS1 aa 391-441. The exact sequence is proprietary. (NP_005229.1).
Database link: P14921
- WB: Jurkat whole cell lysate. IP: Jurkat whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab225868 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 50 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
Sequence similaritiesBelongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain.
modificationsSumoylated on Lys-15 and Lys-227, preferentially by SUMO2; which inhibits transcriptional activity.
Ubiquitinated; which induces proteasomal degradation.
- Information by UniProt
- Avian erythroblastosis virus E26 (v ets) oncogene homolog 1 antibody
- C ets 1 protein antibody
- c-ets-1 antibody
All lanes : Anti-ETS1 antibody (ab225868) at 0.1 µg/ml
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 2 : Jurkat whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 50 kDa
Exposure time: 3 minutes
ETS1 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225868 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225868 at 1 µg/ml.
Lane 1: ab225868 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab225868 has been referenced in 1 publication.
- Xu H et al. Long non-coding RNA PAXIP1-AS1 facilitates cell invasion and angiogenesis of glioma by recruiting transcription factor ETS1 to upregulate KIF14 expression. J Exp Clin Cancer Res 38:486 (2019). PubMed: 31823805