Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab26096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Transcription factor.
  • Sequence similarities
    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
    Contains 1 PNT (pointed) domain.
  • Post-translational
    modifications
    Sumoylated on Lys-15 and Lys-227, preferentially by SUMO2; which inhibits transcriptional activity.
    Ubiquitinated; which induces proteasomal degradation.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Avian erythroblastosis virus E26 (v ets) oncogene homolog 1 antibody
    • C ets 1 protein antibody
    • c-ets-1 antibody
    • ETS 1 antibody
    • Ets protein antibody
    • ETS proto-oncogene 1, transcription factor antibody
    • ETS1 antibody
    • ETS1 oncogene antibody
    • ETS1 protein antibody
    • ETS1_HUMAN antibody
    • EWSR 2 antibody
    • EWSR2 antibody
    • FLJ10768 antibody
    • Oncogene ETS1 antibody
    • P54 antibody
    • Protein C-ets-1 antibody
    • v ets avian erythroblastosis virus E2 oncogene homolog antibody
    • v ets avian erythroblastosis virus E2 oncogene homolog 1 antibody
    • v ets avian erythroblastosis virus E26 oncogene homolog 1 antibody
    • v ets erythroblastosis virus E26 oncogene homolog 1 antibody
    • v-ets erythroblastosis virus E26 oncogene homolog 1 antibody
    see all

Images

  • Anti-ETS1 antibody (ab26096) at 5 µg/ml + HepG2 cell lysate

    Predicted band size: 50 kDa
    Observed band size: 56 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling ETS1 with ab26096 at a concentration of 4-8µg/ml. The arrows indicate positively labelled epithelial cells of pancreatic acini. Magnification: 400X.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling ETS1 with ab26096 at a concentration of 4-8µg/ml. Positively labelled epithelial cells of renal tubules are indicated by arrows. Magnification: 400X.

  • ICC/IF image of ab26096 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26096, 10µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Zhang Y  et al. ETS1 is associated with cisplatin resistance through IKKa/NF-?B pathway in cell line MDA-MB-231. Cancer Cell Int 18:86 (2018). Read more (PubMed: 29950928) »
  • Wu S  et al. Novel variants in MLL confer to bladder cancer recurrence identified by whole-exome sequencing. Oncotarget 7:2629-45 (2016). Read more (PubMed: 26625313) »
See all 7 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us.

The antibody ab26096 has not been tested in Chip application so I am sorry we are unable to confirm its use. This however does not means the antibody cannot be used it only means we do not have tested data. If you are interested in testing this antibody then I would recommend availing the 100% Abtrial discount, please click the link below for more information;

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Thank you for your enquiry. The method used with ab26096 was heat-mediated antigen retrieval with citrate buffer pH 6.0. Please do not hesitate to contact us if you need anything further.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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