RecombinantRabMAb

Recombinant Anti-ETS1 antibody [EPR21909] (ab220361)

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Western blot - Anti-ETS1 antibody [EPR21909] (ab220361)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (ab220361)
  • Western blot - Anti-ETS1 antibody [EPR21909] (ab220361)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (ab220361)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (ab220361)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (ab220361)
  • Anti-ETS1 antibody [EPR21909] (ab220361)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21909] to ETS1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-ETS1 antibody [EPR21909]
    See all ETS1 primary antibodies

  • Description

    Rabbit monoclonal [EPR21909] to ETS1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human and mouse lymph node lysates; KARPAS-299 and C6 whole cell lysates; Rat lung and spleen lysates. IHC-P: Human tonsil and Hodgkin's lymphoma tissues; Mouse thymus tissue; Rat spleen tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab220361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

This antibody has low affinity in WB. We suggest large amount of samples to be loaded or a high sensitive WB experiment, such as super-sensitive ECL substrate, to be used with this antibody.  
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

This antibody has low affinity in WB. We suggest large amount of samples to be loaded or a high sensitive WB experiment, such as super-sensitive ECL substrate, to be used with this antibody.  
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Transcription factor.

  • Sequence similarities

    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
    Contains 1 PNT (pointed) domain.

  • Post-translational
    modifications

    Sumoylated on Lys-15 and Lys-227, preferentially by SUMO2; which inhibits transcriptional activity.
    Ubiquitinated; which induces proteasomal degradation.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Avian erythroblastosis virus E26 (v ets) oncogene homolog 1 antibody
    • C ets 1 protein antibody
    • c-ets-1 antibody
    • ETS 1 antibody
    • Ets protein antibody
    • ETS proto-oncogene 1, transcription factor antibody
    • ETS1 antibody
    • ETS1 oncogene antibody
    • ETS1 protein antibody
    • ETS1_HUMAN antibody
    • EWSR 2 antibody
    • EWSR2 antibody
    • FLJ10768 antibody
    • Oncogene ETS1 antibody
    • P54 antibody
    • Protein C-ets-1 antibody
    • v ets avian erythroblastosis virus E2 oncogene homolog antibody
    • v ets avian erythroblastosis virus E2 oncogene homolog 1 antibody
    • v ets avian erythroblastosis virus E26 oncogene homolog 1 antibody
    • v ets erythroblastosis virus E26 oncogene homolog 1 antibody
    • v-ets erythroblastosis virus E26 oncogene homolog 1 antibody
    see all

Images

  • Western blot - Anti-ETS1 antibody [EPR21909] (ab220361)
    Western blot - Anti-ETS1 antibody [EPR21909] (ab220361)
    All lanes : Anti-ETS1 antibody [EPR21909] (ab220361) at 1/1000 dilution

    Lane 1 : KARPAS-299 (human anaplastic large cell lymphoma cell line) whole cell lysate at 20 µg
    Lane 2 : Rat lung lysate at 20 µg
    Lane 3 : Rat spleen lysate at 20 µg
    Lane 4 : C6 (rat glial tumor cell line) whole cell lysate at 10 µg
    Lane 5 : Mouse lymph node lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa



    Exposure time : Lane 1: 125 seconds; Lanes 2,3 and 4: 3 minutes; Lane 5: 70 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate. This antibody has low affinity in WB. We suggest large amount of samples to be loaded or a high sensitive WB experiment, such as super-sensitive ECL substrate, to be used with this antibody.  

    Lower weak bands below 50 kDa represent other ETS1 isoforms.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (ab220361)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (ab220361)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling ETS1 with ab220361 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on human tonsil (PMID: 23288305; PMID: 10377039). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    We recommend that customers optimize their experimental conditions when testing on human spleen and thymus tissues.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-ETS1 antibody [EPR21909] (ab220361)
    Western blot - Anti-ETS1 antibody [EPR21909] (ab220361)
    Anti-ETS1 antibody [EPR21909] (ab220361) at 1/1000 dilution + Human lymph node lysate at 10 µg

    Secondary
    VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate. This antibody has low affinity in WB. We suggest large amount of samples to be loaded or a high sensitive WB experiment, such as super-sensitive ECL substrate, to be used with this antibody.  

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (ab220361)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (ab220361)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling ETS1 with ab220361 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on rat spleen (PMID: 23288305; PMID: 10377039). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (ab220361)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (ab220361)

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling ETS1 with ab220361 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on mouse thymus (PMID: 22307076; PMID: 3472202). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (ab220361)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (ab220361)

    Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue labeling ETS1 with ab220361 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on human Hodgkin’s lymphoma (PMID: 23288305; PMID: 10377039). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    We recommend that customers optimize their experimental conditions when testing on human spleen and thymus tissues.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-ETS1 antibody [EPR21909] (ab220361)
    Anti-ETS1 antibody [EPR21909] (ab220361)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (5)

Publishing research using ab220361? Please let us know so that we can cite the reference in this datasheet.

ab220361 has been referenced in 5 publications.

  • Li R  et al. ETS Proto-Oncogene 1 Suppresses MicroRNA-128 Transcription to Promote Osteogenic Differentiation Through the HOXA13/ß-Catenin Axis. Front Physiol 12:626248 (2021). PubMed: 33746773
  • Zhu Y  et al. Methylation status of CpG sites in the NOTCH4 promoter region regulates NOTCH4 expression in patients with tetralogy of Fallot. Mol Med Rep 22:4412-4422 (2020). PubMed: 33000281
  • Liu J  et al. Histone Demethylase KDM3A Promotes Cervical Cancer Malignancy Through the ETS1/KIF14/Hedgehog Axis. Onco Targets Ther 13:11957-11973 (2020). PubMed: 33239895
  • Dai J  et al. Long Noncoding RNA LINC01426 Sequesters microRNA-519d-5p to Promote Non-Small Cell Lung Cancer Progression by Increasing ETS1 Expression. Cancer Manag Res 12:12697-12708 (2020). PubMed: 33335425
  • Li Y  et al. Long Non-coding RNA LINC01787 Drives Breast Cancer Progression via Disrupting miR-125b Generation. Front Oncol 9:1140 (2019). PubMed: 31750242

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