Recombinant Anti-ETS1 antibody [EPR21909] (ab220361)
![Western blot - Anti-ETS1 antibody [EPR21909] (ab220361)](https://www.abcam.com/ps/products/220/ab220361/Images/ab220361-316598-anti-ets1-antibody-epr21909-western-blot.jpg "Western blot - Anti-ETS1 antibody [EPR21909] (ab220361)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21909] to ETS1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-ETS1 antibody [EPR21909]
See all ETS1 primary antibodies -
Description
Rabbit monoclonal [EPR21909] to ETS1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseRatHumanWB MouseRatHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human and mouse lymph node lysates; KARPAS-299 and C6 whole cell lysates; Rat lung and spleen lysates. IHC-P: Human tonsil and Hodgkin's lymphoma tissues; Mouse thymus tissue; Rat spleen tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21909 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab220361 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ChIP |
Human
|
| IHC-P |
Mouse
Rat
Human
|
| WB |
Mouse
Rat
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
This antibody has low affinity in WB. We suggest large amount of samples to be loaded or a high sensitive WB experiment, such as super-sensitive ECL substrate, to be used with this antibody. |
|
| IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). This antibody has low affinity in WB. We suggest large amount of samples to be loaded or a high sensitive WB experiment, such as super-sensitive ECL substrate, to be used with this antibody. |
|
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Transcription factor. -
Sequence similarities
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain. -
Post-translational
modificationsSumoylated on Lys-15 and Lys-227, preferentially by SUMO2; which inhibits transcriptional activity.
Ubiquitinated; which induces proteasomal degradation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2113 Human
- Entrez Gene: 23871 Mouse
- Entrez Gene: 24356 Rat
- Omim: 164720 Human
- SwissProt: P14921 Human
- SwissProt: P27577 Mouse
- SwissProt: P41156 Rat
- Unigene: 369438 Human
see all -
Alternative names
- Avian erythroblastosis virus E26 (v ets) oncogene homolog 1 antibody
- C ets 1 protein antibody
- c-ets-1 antibody
see all
Images
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Western blot - Anti-ETS1 antibody [EPR21909] (ab220361)All lanes : Anti-ETS1 antibody [EPR21909] (ab220361) at 1/1000 dilution
Lane 1 : KARPAS-299 (human anaplastic large cell lymphoma cell line) whole cell lysate at 20 µg
Lane 2 : Rat lung lysate at 20 µg
Lane 3 : Rat spleen lysate at 20 µg
Lane 4 : C6 (rat glial tumor cell line) whole cell lysate at 10 µg
Lane 5 : Mouse lymph node lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDaExposure time : Lane 1: 125 seconds; Lanes 2,3 and 4: 3 minutes; Lane 5: 70 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate. This antibody has low affinity in WB. We suggest large amount of samples to be loaded or a high sensitive WB experiment, such as super-sensitive ECL substrate, to be used with this antibody.
Lower weak bands below 50 kDa represent other ETS1 isoforms.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (ab220361)](https://www.abcam.com/ps/products/220/ab220361/Images/ab220361-316593-anti-ets1-antibody-epr21909-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (ab220361)Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling ETS1 with ab220361 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on human tonsil (PMID: 23288305; PMID: 10377039). Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
We recommend that customers optimize their experimental conditions when testing on human spleen and thymus tissues.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Western blot - Anti-ETS1 antibody [EPR21909] (ab220361)](https://www.abcam.com/ps/products/220/ab220361/Images/ab220361-316597-anti-ets1-antibody-epr21909-western-blot.jpg)
Western blot - Anti-ETS1 antibody [EPR21909] (ab220361)Anti-ETS1 antibody [EPR21909] (ab220361) at 1/1000 dilution + Human lymph node lysate at 10 µg
Secondary
VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate. This antibody has low affinity in WB. We suggest large amount of samples to be loaded or a high sensitive WB experiment, such as super-sensitive ECL substrate, to be used with this antibody.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (ab220361)](https://www.abcam.com/ps/products/220/ab220361/Images/ab220361-316596-anti-ets1-antibody-epr21909-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (ab220361)Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling ETS1 with ab220361 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on rat spleen (PMID: 23288305; PMID: 10377039). Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (ab220361)](https://www.abcam.com/ps/products/220/ab220361/Images/ab220361-316595-anti-ets1-antibody-epr21909-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (ab220361)Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling ETS1 with ab220361 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on mouse thymus (PMID: 22307076; PMID: 3472202). Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (ab220361)](https://www.abcam.com/ps/products/220/ab220361/Images/ab220361-316594-anti-ets1-antibody-epr21909-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (ab220361)Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue labeling ETS1 with ab220361 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on human Hodgkin’s lymphoma (PMID: 23288305; PMID: 10377039). Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
We recommend that customers optimize their experimental conditions when testing on human spleen and thymus tissues.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-ETS1 antibody [EPR21909] (ab220361)](https://www.abcam.com/ps/products/220/ab220361/Images/ab220361-7-benefits-of-recombinant-antibodies.png)
Anti-ETS1 antibody [EPR21909] (ab220361)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab220361 has been referenced in 1 publication.
- Li Y et al. Long Non-coding RNA LINC01787 Drives Breast Cancer Progression via Disrupting miR-125b Generation. Front Oncol 9:1140 (2019). PubMed: 31750242