Key features and details
- Mouse monoclonal [mAbcam63302] to ETS1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ETS1 antibody [mAbcam63302]
See all ETS1 primary antibodies
DescriptionMouse monoclonal [mAbcam63302] to ETS1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Pig
Recombinant fragment corresponding to Human ETS1 aa 100-250.
(Peptide available as
- Purchase matching WB positive control:Recombinant Human ETS1 protein
- This antibody gave a positive signal in the following human lysates: HepG2 Nuclear; Jurkat Nuclear; HeLa Nuclear; MCF7 Whole Cell.
This antibody clone is manufactured by Abcam.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Buffer: 1x PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab63302 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 10 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).|
Sequence similaritiesBelongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain.
modificationsSumoylated on Lys-15 and Lys-227, preferentially by SUMO2; which inhibits transcriptional activity.
Ubiquitinated; which induces proteasomal degradation.
- Information by UniProt
- Avian erythroblastosis virus E26 (v ets) oncogene homolog 1 antibody
- C ets 1 protein antibody
- c-ets-1 antibody
All lanes : Anti-ETS1 antibody [mAbcam63302] (ab63302) at 10 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate
Lane 2 : Jurkat nuclear extract lysate (ab14844)
Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 55,60 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa (possible isoform)
Exposure time: 2 minutes
Ab63302 is predicted to react with both isoforms of ETS1. c-ETS-1A has a predicted molecular weight of 50-kDa, whereas c-ETS-1B has a predicted molecular weight of 41-kDa. The ETS1 protein sequence contains several phosphorylation sites (Swissprot), which might explain the banding pattern observed.
ab63302 has not yet been referenced specifically in any publications.