Anti-ETS1 antibody [mAbcam63302] (ab63302)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-ETS1 antibody [mAbcam63302]
See all ETS1 primary antibodies -
Description
Mouse monoclonal [mAbcam63302] to ETS1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Pig
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Immunogen
Recombinant fragment corresponding to Human ETS1 aa 100-250.
(Peptide available asab101223) -
Positive control
- Purchase matching WB positive control:Recombinant Human ETS1 protein
- This antibody gave a positive signal in the following human lysates: HepG2 Nuclear; Jurkat Nuclear; HeLa Nuclear; MCF7 Whole Cell.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Buffer: 1x PBS -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
mAbcam63302 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab63302 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 10 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa). |
Target
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Function
Transcription factor. -
Sequence similarities
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain. -
Post-translational
modificationsSumoylated on Lys-15 and Lys-227, preferentially by SUMO2; which inhibits transcriptional activity.
Ubiquitinated; which induces proteasomal degradation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2113 Human
- Entrez Gene: 23871 Mouse
- Entrez Gene: 24356 Rat
- Omim: 164720 Human
- SwissProt: P14921 Human
- SwissProt: P27577 Mouse
- SwissProt: P41156 Rat
- Unigene: 369438 Human
see all -
Alternative names
- Avian erythroblastosis virus E26 (v ets) oncogene homolog 1 antibody
- C ets 1 protein antibody
- c-ets-1 antibody
see all
Images
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![Western blot - Anti-ETS1 antibody [mAbcam63302] (ab63302)](https://www.abcam.com/ps/products/63/ab63302/Images/ab63302-76544-anti-ets1-antibody-mabcam63302-western-blot.jpg)
Western blot - Anti-ETS1 antibody [mAbcam63302] (ab63302)All lanes : Anti-ETS1 antibody [mAbcam63302] (ab63302) at 10 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate
Lane 2 :Jurkat nuclear extract lysate (ab14844)
Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 55,60 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa (possible isoform)
Exposure time: 2 minutes
Ab63302 is predicted to react with both isoforms of ETS1. c-ETS-1A has a predicted molecular weight of 50-kDa, whereas c-ETS-1B has a predicted molecular weight of 41-kDa. The ETS1 protein sequence contains several phosphorylation sites (Swissprot), which might explain the banding pattern observed.
Protocols
Datasheets and documents
References
ab63302 has not yet been referenced specifically in any publications.
