Application
Western blot
Loading amount
32 µg
Gel Running Conditions
Reduced Denaturing (10% gel, semi-dry transfer)
Sample
Chicken Cell lysate - whole cell (LMH cells overexpressing chicken Ets1A and B)
Specification
LMH cells overexpressing chicken Ets1A and B
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS/0.1%Tween 20/1%milk
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: anti-mouse IgG HRP-linked antibody
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2500
Detection
Detection method
20xLumiGlo
Bands
Specific: ~50, ~55 kDa Non-specific: ~25 kDa
Exposure
2 minute(s) and 30 second(s)
Additional data
Additional Notes
Samples are identified as follow:
1 LMH cells not transfected
2 LMH transfected with empty vector
3. LMH transfected with chicken Ets1A
4. LMH transfected with chicken Ets1B
5 empty lane
Abcam user community
Verified customer
Submitted Feb 21 2014