Product nameAnti-ETS1 (phospho T38) antibody
See all ETS1 primary antibodies
DescriptionRabbit polyclonal to ETS1 (phospho T38)
SpecificityDetects endogenous levels of ETS1 only when phosphorylated at threonine 38.
Tested applicationsSuitable for: IHC-P, ELISA, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic phosphopeptide from around the phosphorylation site of threonine 38 (LLTPPS) of human ETS1
- Human breast carcinoma tissue. IF/ICC: HepG2 cell line.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAffinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab59179 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 50 kDa).|
Sequence similaritiesBelongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain.
modificationsSumoylated on Lys-15 and Lys-227, preferentially by SUMO2; which inhibits transcriptional activity.
Ubiquitinated; which induces proteasomal degradation.
- Information by UniProt
- Avian erythroblastosis virus E26 (v ets) oncogene homolog 1 antibody
- C ets 1 protein antibody
- c-ets-1 antibody
All lanes : Anti-ETS1 (phospho T38) antibody (ab59179) at 1/1000 dilution
Lane 1 : Human A2058 whole cell lysate
Lane 2 : Human A375 whole cell lysate
Lysates/proteins at 50 µg per lane.
All lanes : HRP-conjugated Hourse anti-rabbit IgG polyclonal at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa (possible non-specific binding), 180 kDa (possible non-specific binding), 70 kDa (possible non-specific binding), 90 kDa (possible non-specific binding)
Exposure time: 5 minutes
ICC/IF image of ab59179 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59179, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue using ab59179 at 1/50 dilution. Left: untreated. Right: sample treated with immunising peptide.
This product has been referenced in:
- Yang Z et al. Regulation of cisplatin-resistant head and neck squamous cell carcinoma by the SRC/ETS-1 signaling pathway. BMC Cancer 19:485 (2019). Read more (PubMed: 31118072) »
- Sinh ND et al. Ets1 and ESE1 reciprocally regulate expression of ZEB1/ZEB2, dependent on ERK1/2 activity, in breast cancer cells. Cancer Sci 108:952-960 (2017). Read more (PubMed: 28247944) »