Overview

  • Product name

  • Description

    Rabbit polyclonal to ETS2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Synthetic peptide corresponding to Human ETS2 aa 146-173 conjugated to keyhole limpet haemocyanin.
    Sequence:

    RFLELAPDFVGDILWEHLEQMIKENQEK


    Database link: P15036

  • Positive control

    • IHC-P: Human tonsil tissue. WB: MCF7 cell lysate. Flow Cytometry: MCF7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab230519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/75. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 53 kDa.
Flow Cyt 1/10 - 1/50.

Target

Images

  • Anti-ETS2 antibody (ab230519) at 1/1000 dilution + MCF7 (Human breast adenocarcinoma cell line) cell lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 53 kDa

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for ETS2 with ab230519 at a 1/75 dilution in immunohistochemical analysis.

  • Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma cell line) cells, labeling ETS2 with ab230519 at a 1/10 dilution (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab230519 has not yet been referenced specifically in any publications.

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