Product nameAnti-ETS2 antibody
See all ETS2 primary antibodies
DescriptionRabbit polyclonal to ETS2
Tested applicationsSuitable for: WB, ICC/IF, IHC-P, IPmore details
Species reactivityReacts with: Rat, Human
Recombinant fragment within Human ETS2. The exact sequence is proprietary. Center region.
Database link: P15036
- WB: HeLa whole cell lysate. IHC-P: Rat forebrain tissue. ICC/IF: HeLa cells. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab272866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000.|
|ICC/IF||1/100 - 1/1000.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|IP||1/100 - 1/500.|
Sequence similaritiesBelongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain.
- Information by UniProt
- C ets 2 protein antibody
- ETS2 antibody
- ETS2_HUMAN antibody
Paraffin-embedded rat forebrain tissue stained for ETS2 using ab272866 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ETS2 using ab272866 at 1/500 dilution (Green) in ICC/IF. Alpha-tubulin filaments (Red).
Anti-ETS2 antibody (ab272866) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg
HRP-conjugated anti-rabbit IgG antibody
10% SDS PAGE
IP Sample: HeLa whole cell lysate/extract
All lanes : Anti-ETS2 antibody (ab272866) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Control with 2 µg of preimmune rabbit IgG.
Lane 3 : Immunoprecipitation of ETS2 protein by 2 µg of ab272866
Lysates/proteins at 40 µg per lane.
All lanes : anti-rabbit IgG HRP
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab272866 has not yet been referenced specifically in any publications.