Recombinant Anti-ETS2 antibody [EPR22419] (ab219948)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22419] to ETS2
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-ETS2 antibody [EPR22419]
See all ETS2 primary antibodies -
Description
Rabbit monoclonal [EPR22419] to ETS2 -
Host species
Rabbit -
Specificity
This antibody is not recommended for human in IHC.
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Tested applications
Suitable for: WB, IHC-P, ICC/IF, IPmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: LPS treated RAW 264.7 cell lysate; K562 whole cell lysate; Human thymus lysate. IHC-P: Mouse and rat thymus tissue. ICC/IF: LPS treated RAW 264.7 cells. IP: K562 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22419 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry reagents
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab219948 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 50-55 kDa (predicted molecular weight: 53 kDa).
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IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
1/500.
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IP |
1/30.
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Notes |
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WB
1/1000. Detects a band of approximately 50-55 kDa (predicted molecular weight: 53 kDa). |
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/500. |
IP
1/30. |
Target
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Sequence similarities
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2114 Human
- Entrez Gene: 23872 Mouse
- Entrez Gene: 304063 Rat
- Omim: 164740 Human
- SwissProt: P15036 Human
- SwissProt: P15037 Mouse
- Unigene: 644231 Human
- Unigene: 290207 Mouse
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Alternative names
- C ets 2 protein antibody
- ETS2 antibody
- ETS2_HUMAN antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling ETS2 with ab219948 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining on mouse thymus (PMID: 3472202, PMID:26926107) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -
Immunohistochemical analysis of paraffin-embedded rat thymus tissue labeling ETS2 with ab219948 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining on rat thymus (PMID: 3472202, PMID:26926107) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling ETS2 with ab219948 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining in RAW 264.7 cells treated with lipopolysaccharide (500 ng/ml) for 6 hours. The nuclear counterstain is DAPI (blue).
Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red).
Secondary antibody contril: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution. -
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling ETS2 with ab219948 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Negative control: No staining on rat cerebellum (PMID: 3472202) is observed. Counterstained with hematoxylin.
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling ETS2 with ab219948 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Negative control: No staining on mouse cerebrum (PMID: 3472202) is observed. Counterstained with hematoxylin.
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -
ETS2 was immunoprecipitated from 0.35 mg K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate with ab219948 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab219948 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: K562 whole cell lysate 10 µg (Input).
Lane 2: ab219948 IP in K562 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab219948 in K562 whole cell lysate.Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.The two immunoprecipitated bands represent non-phosphorylated and phosphorylated ETS2.
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All lanes : Anti-ETS2 antibody [EPR22419] (ab219948) at 1/1000 dilution
Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 20 µg
Lane 2 : Human thymus at 10 µg
Secondary
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 53 kDa
Observed band size: 53 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 26 seconds, Lane 2: 3 minutes.
Lane 1 was developed using a higher sensitivity ECL substrate.
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All lanes : Anti-ETS2 antibody [EPR22419] (ab219948) at 1/1000 dilution
Lane 1 : Untreated RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
Lane 2 : RAW 264.7 treated with 500ng/ml lipopolysaccharides(LPS) for 6 hours
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 53 kDa
Observed band size: 50-55 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID: 15590657). The doublets represent ETS2 and its phosphorylated form.
This blot was developed using a higher sensitivity ECL substrate.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab219948 has been referenced in 2 publications.
- Gu L et al. TLR13 contributes to skeletal muscle atrophy by increasing insulin resistance in chronic kidney disease. Cell Prolif 55:e13181 (2022). PubMed: 35088922
- Feng D et al. Chromatin organizer SATB1 controls the cell identity of CD4+ CD8+ double-positive thymocytes by regulating the activity of super-enhancers. Nat Commun 13:5554 (2022). PubMed: 36138028