Overview

  • Product name

    Anti-ETS2 antibody [OTI1H4]
    See all ETS2 primary antibodies
  • Description

    Mouse monoclonal [OTI1H4] to ETS2
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 90-349 of Human ETS2 produced in E.coli (NP_005230).

  • Positive control

    • HEK293T cells transfected with pCMV6-ENTRY ETS2 cDNA; Human pancreas, thyroid carcinoma, endometrium adenocarcinoma and tonsil tissues.
  • General notes

    The clone number has been updated from 1H4 to OTI1H4, both clone numbers name the same clone.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

Properties

Applications

Our Abpromise guarantee covers the use of ab140371 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/4000. Predicted molecular weight: 53 kDa.
IHC-P 1/150.

Target

  • Sequence similarities

    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
    Contains 1 PNT (pointed) domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • C ets 2 protein antibody
    • ETS2 antibody
    • ETS2_HUMAN antibody
    • ETS2IT1 antibody
    • Oncogene ETS 2 antibody
    • Oncogene ETS2 antibody
    • Protein C-ets-2 antibody
    • v ets avian erythroblastosis virus E2 oncogene homolog 2 antibody
    • V ets avian erythroblastosis virus E26 oncogene homolog 2 antibody
    • V ets erythroblastosis virus E26 oncogene homolog 2 antibody
    see all

Images

  • All lanes : Anti-ETS2 antibody [OTI1H4] (ab140371) at 1/4000 dilution

    Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cells transfected with pCMV6-ENTRY ETS2 cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 53 kDa



    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC208418)
  • Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labelling ETS2 with ab140371 at 1/150 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labelling ETS2 with ab140371 at 1/150 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human endometrium adenocarcinoma tissue labelling ETS2 with ab140371 at 1/150 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling ETS2 with ab140371 at 1/150 dilution.

References

ab140371 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
10% FBS - 3% BSA in PBS 1X as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Human Cell (Jurkat cells)
Specification
Jurkat cells
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde

Mr. Karagiannis Fotis

Verified customer

Submitted Jul 22 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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