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v ets avian erythroblastosis virus E2 oncogene homolog 2 antibody
V ets avian erythroblastosis virus E26 oncogene homolog 2 antibody
V ets erythroblastosis virus E26 oncogene homolog 2 antibody
Western blot - Anti-ETS2 (phospho T72) antibody (ab153653)
All lanes : Anti-ETS2 (phospho T72) antibody (ab153653) at 1 µg/ml
Lane 1 : Human kidney tissue lysate - total protein (ab30203) Lane 2 : Human kidney tissue lysate - total protein (ab30203) with Immunising peptide at 1 µg/ml Lane 3 : Human kidney tissue lysate - total protein (ab30203) with Control peptide at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
The predicted molecular weight of ETS2 is 53 kDa (SwissProt), however we expect to observe a banding pattern at 70 kDa.
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab153653 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab153653 was tested using an Indirect ELISA approach. The wells were coated with peptide (1µg/ml at 100µl/well) overnight at 4°C, followed by a 5% BSA blocking step for 1 hour at room temperature. The primary Ab was then added at a dilution range of 1- 0.00025µg/ml (100µl/well) for 1hr at room temperature. A HRP-conjugated anti-rabbit IgG (heavy and light chain) was used as a secondary antibody at 1:20,000 dilution for 1hr at room temperature.