Overview

  • Product name

    Anti-ETV2 antibody [EPR5229(3)]
    See all ETV2 primary antibodies
  • Description

    Rabbit monoclonal [EPR5229(3)] to ETV2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ETV2 aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: O00321

  • Positive control

    • HUVEC cell lysate of lent-virally expressed Human ETV2 and Human bone marrow stromal cell lysate lent-virally expressed mouse ETV2. HUVEC cells expressing exogenous Human ETV2.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 37 kDa).
ICC/IF 1/500.

Target

  • Relevance

    ETV2 is a member of the ETS family of transcription factors. It has been proposed that ETV2 acts downstream of BMP, notch and wnt signalling in blood and vessel specification. ER71 has also shown to be important for Flk1 expression as well as hematopoietic and vascular development.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • ER71 antibody
    • Ets related protein 71 antibody
    • ETS translocation variant 2 antibody
    • Ets variant gene 2 antibody
    • Ets-related protein 71 antibody
    • Ets-related protein 71, mouse, homolog of antibody
    • ETSRP71 antibody
    see all

Images

  • All lanes : Anti-ETV2 antibody [EPR5229(3)] (ab181847) at 1/10000 dilution

    Lane 1 : HUVEC cell lysate
    Lane 2 : HUVEC cell lysate of lent-virally expressed Human ETV2
    Lane 3 : Human bone marrow stromal cell lysate
    Lane 4 : Human bone marrow stromal cell lysate lent-virally expressed mouse ETV2

    Developed using the ECL technique.

    Predicted band size: 37 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?

  • Immunofluorescence analysis of paraformaldehyde-fixed HUVEC cells with exogenous Human ETV2 (upper panels) or without (lower panels), labeling ETV2 with ab181847 at a 1/500 dilution. As secondary antibody goat anti rabbit IgG (Alexa Fluor®488) was used at a 1/200. In blue DAPI staining.

References

This product has been referenced in:

  • Kabir AU  et al. Requisite endothelial reactivation and effective siRNA nanoparticle targeting of Etv2/Er71 in tumor angiogenesis. JCI Insight 3:N/A (2018). Read more (PubMed: 29669933) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181847.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up