Recombinant Anti-ETV2 antibody [EPR5229(3)] - BSA and Azide free (ab250521)
![Immunocytochemistry/ Immunofluorescence - Anti-ETV2 antibody [EPR5229(3)] - BSA and Azide free (ab250521)](https://www.abcam.com/ps/products/250/ab250521/Images/ab250521-355818-anti-etv2-antibody-epr5229-3-immunocytochemistry-immunfluorescence-huvec-human.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ETV2 antibody [EPR5229(3)] - BSA and Azide free (ab250521)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5229(3)] to ETV2 - BSA and Azide free
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-ETV2 antibody [EPR5229(3)] - BSA and Azide free
See all ETV2 primary antibodies -
Description
Rabbit monoclonal [EPR5229(3)] to ETV2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: HUVEC cells with exogenous Human ETV2.
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General notes
ab250521 is the carrier-free version of ab181847.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5229(3) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250521 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 40 kDa (predicted molecular weight: 37 kDa).
|
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| ICC/IF |
Use at an assay dependent concentration.
|
| Notes |
|---|
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WB
Use at an assay dependent concentration. Detects a band of approximately 40 kDa (predicted molecular weight: 37 kDa). |
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ICC/IF
Use at an assay dependent concentration. |
Target
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Relevance
ETV2 is a member of the ETS family of transcription factors. It has been proposed that ETV2 acts downstream of BMP, notch and wnt signalling in blood and vessel specification. ER71 has also shown to be important for Flk1 expression as well as hematopoietic and vascular development. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 2116 Human
- Entrez Gene: 14008 Mouse
- Omim: 609358 Human
- SwissProt: O00321 Human
- SwissProt: P41163 Mouse
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Alternative names
- ER71 antibody
- Ets related protein 71 antibody
- ETS translocation variant 2 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ETV2 antibody [EPR5229(3)] - BSA and Azide free (ab250521)
Immunofluorescence analysis of paraformaldehyde-fixed HUVEC cells with exogenous Human ETV2 (upper panels) or without (lower panels), labeling ETV2 with ab181847 at a 1/500 dilution. As secondary antibody goat anti rabbit IgG (Alexa Fluor®488) was used at a 1/200. In blue DAPI staining.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181847).
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![Anti-ETV2 antibody [EPR5229(3)] - BSA and Azide free (ab250521)](https://www.abcam.com/ps/products/250/ab250521/Images/ab250521-2-benefits-of-recombinant-antibodies.png)
Anti-ETV2 antibody [EPR5229(3)] - BSA and Azide free (ab250521)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab250521 has not yet been referenced specifically in any publications.
