Recombinant Anti-ETV3 antibody [EPR8658] (ab154810)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8658] to ETV3
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ETV3 antibody [EPR8658]
See all ETV3 primary antibodies -
Description
Rabbit monoclonal [EPR8658] to ETV3 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human ETV3. The exact sequence is proprietary.
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Positive control
- HepG2, HeLa, SH-SY5Y and U937 cell lysates; Hela cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8658 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154810 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 57 kDa.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/50000. Predicted molecular weight: 57 kDa. |
Target
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Function
Transcriptional repressor that contribute to growth arrest during terminal macrophage differentiation by repressing target genes involved in Ras-dependent proliferation. Represses MMP1 promoter activity. -
Sequence similarities
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2117 Human
- Entrez Gene: 27049 Mouse
- Entrez Gene: 295297 Rat
- Omim: 164873 Human
- SwissProt: P41162 Human
- SwissProt: Q8R4Z4 Mouse
- Unigene: 105636 Human
- Unigene: 219460 Mouse
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Alternative names
- AI414410 antibody
- bA110J1.4 antibody
- ETS domain transcriptional repressor PE1 antibody
see all
Images
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All lanes : Anti-ETV3 antibody [EPR8658] (ab154810) at 1/10000 dilution
Lane 1 : HepG2 lysate
Lane 2 : HeLa lysate
Lane 3 : SHSY5Y lysate
Lane 4 : U937 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 57 kDa -
Intracellular flow cytometric analysis of permeabilized Hela cells labeling ETV3 with ab154810 at 1/10 dilution (red) or a negative rabbit IgG (control) (green).
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab154810 has not yet been referenced specifically in any publications.