Anti-ETV3 antibody [EPR8658] Recombinant (ab154810)

Product name

Anti-ETV3 antibody [EPR8658]
See all ETV3 primary antibodies
Description

Rabbit monoclonal [EPR8658] to ETV3
Host species

Rabbit
Tested applications

Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human ETV3. The exact sequence is proprietary.
Positive control
- HepG2, HeLa, SH-SY5Y and U937 cell lysates; Hela cells.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR8658
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab154810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/10000 - 1/50000. Predicted molecular weight: 57 kDa.
Flow Cyt		1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Application

Abreviews

Notes

WB

1/10000 - 1/50000. Predicted molecular weight: 57 kDa.

Flow Cyt

1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Application notes

Is unsuitable for ICC/IF,IHC-P or IP.

Function

Transcriptional repressor that contribute to growth arrest during terminal macrophage differentiation by repressing target genes involved in Ras-dependent proliferation. Represses MMP1 promoter activity.
Sequence similarities

Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Cellular localization

Nucleus.
Target information above from: UniProt accession P41162 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 2117 Human
- Entrez Gene: 27049 Mouse
- Entrez Gene: 295297 Rat
- Omim: 164873 Human
- SwissProt: P41162 Human
- SwissProt: Q8R4Z4 Mouse
- Unigene: 105636 Human
- Unigene: 219460 Mouse
- Unigene: 225088 Rat
see all
Alternative names
- AI414410 antibody
- bA110J1.4 antibody
- ETS domain transcriptional repressor PE1 antibody
- ETS family transcriptional repressor antibody
- ETS translocation variant 3 antibody
- ets variant 3 antibody
- ets variant gene 3 antibody
- ets variant gene 3, ETS family transcriptional repressor antibody
- ETV3 antibody
- ETV3_HUMAN antibody
- ME PE1 antibody
- METS antibody
- Mitogenic Ets transcriptional suppressor antibody
- Oncogene PE1 antibody
- PE 1 antibody
- PE-1 antibody
- PE1 antibody
see all

Western blot - Anti-ETV3 antibody [EPR8658] (ab154810)

All lanes : Anti-ETV3 antibody [EPR8658] (ab154810) at 1/10000 dilution

Lane 1 : HepG2 lysate
Lane 2 : HeLa lysate
Lane 3 : SHSY5Y lysate
Lane 4 : U937 lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 57 kDa
Flow Cytometry - Anti-ETV3 antibody [EPR8658] (ab154810)

Flow cytometric analysis of permeabilized Hela cells labeling ETV3 with ab154810 at 1/10 dilution (red) or a negative rabbit IgG (control) (green).

ab154810 has not yet been referenced specifically in any publications.

Publishing research using ab154810? Please let us know so that we can cite the reference in this datasheet.

Anti-ETV3 antibody [EPR8658] (ab154810)