RecombinantRabMAb

Recombinant Anti-ETV3 antibody [EPR8658] (ab154810)

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Western blot - Anti-ETV3 antibody [EPR8658] (ab154810)
  • Flow Cytometry (Intracellular) - Anti-ETV3 antibody [EPR8658] (ab154810)
  • Anti-ETV3 antibody [EPR8658] (ab154810)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8658] to ETV3
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-ETV3 antibody [EPR8658]
    See all ETV3 primary antibodies

  • Description

    Rabbit monoclonal [EPR8658] to ETV3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human ETV3. The exact sequence is proprietary.

  • Positive control

    • HepG2, HeLa, SH-SY5Y and U937 cell lysates; Hela cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/50000. Predicted molecular weight: 57 kDa.
Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/50000. Predicted molecular weight: 57 kDa.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.

    • Target

    • Function

      Transcriptional repressor that contribute to growth arrest during terminal macrophage differentiation by repressing target genes involved in Ras-dependent proliferation. Represses MMP1 promoter activity.

    • Sequence similarities

      Belongs to the ETS family.
      Contains 1 ETS DNA-binding domain.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • AI414410 antibody
        • bA110J1.4 antibody
        • ETS domain transcriptional repressor PE1 antibody
        • ETS family transcriptional repressor antibody
        • ETS translocation variant 3 antibody
        • ets variant 3 antibody
        • ets variant gene 3 antibody
        • ets variant gene 3, ETS family transcriptional repressor antibody
        • ETV3 antibody
        • ETV3_HUMAN antibody
        • ME PE1 antibody
        • METS antibody
        • Mitogenic Ets transcriptional suppressor antibody
        • Oncogene PE1 antibody
        • PE 1 antibody
        • PE-1 antibody
        • PE1 antibody
        see all

      Images

      • Western blot - Anti-ETV3 antibody [EPR8658] (ab154810)
        Western blot - Anti-ETV3 antibody [EPR8658] (ab154810)
        All lanes : Anti-ETV3 antibody [EPR8658] (ab154810) at 1/10000 dilution

        Lane 1 : HepG2 lysate
        Lane 2 : HeLa lysate
        Lane 3 : SHSY5Y lysate
        Lane 4 : U937 lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 57 kDa

      • Flow Cytometry (Intracellular) - Anti-ETV3 antibody [EPR8658] (ab154810)
        Flow Cytometry (Intracellular) - Anti-ETV3 antibody [EPR8658] (ab154810)
        Flow cytometric analysis of permeabilized Hela cells labeling ETV3 with ab154810 at 1/10 dilution (red) or a negative rabbit IgG (control) (green).
      • Anti-ETV3 antibody [EPR8658] (ab154810)
        Anti-ETV3 antibody [EPR8658] (ab154810)

      References (0)

      Publishing research using ab154810? Please let us know so that we can cite the reference in this datasheet.

      ab154810 has not yet been referenced specifically in any publications.

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