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RecombinantRabMAb

Recombinant Anti-ETV3 antibody [EPR8658] (ab154810)

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Western blot - Anti-ETV3 antibody [EPR8658] (ab154810)
  • Flow Cytometry (Intracellular) - Anti-ETV3 antibody [EPR8658] (ab154810)
  • Anti-ETV3 antibody [EPR8658] (ab154810)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8658] to ETV3
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-ETV3 antibody [EPR8658]
    See all ETV3 primary antibodies

  • Description

    Rabbit monoclonal [EPR8658] to ETV3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human ETV3. The exact sequence is proprietary.

  • Positive control

    • HepG2, HeLa, SH-SY5Y and U937 cell lysates; Hela cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/50000. Predicted molecular weight: 57 kDa.
Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/50000. Predicted molecular weight: 57 kDa.
Application notes
Is unsuitable for ICC/IF,IHC-P or IP.

Target

  • Function

    Transcriptional repressor that contribute to growth arrest during terminal macrophage differentiation by repressing target genes involved in Ras-dependent proliferation. Represses MMP1 promoter activity.

  • Sequence similarities

    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • AI414410 antibody
      • bA110J1.4 antibody
      • ETS domain transcriptional repressor PE1 antibody
      • ETS family transcriptional repressor antibody
      • ETS translocation variant 3 antibody
      • ets variant 3 antibody
      • ets variant gene 3 antibody
      • ets variant gene 3, ETS family transcriptional repressor antibody
      • ETV3 antibody
      • ETV3_HUMAN antibody
      • ME PE1 antibody
      • METS antibody
      • Mitogenic Ets transcriptional suppressor antibody
      • Oncogene PE1 antibody
      • PE 1 antibody
      • PE-1 antibody
      • PE1 antibody
      see all

    Images

    • Western blot - Anti-ETV3 antibody [EPR8658] (ab154810)
      Western blot - Anti-ETV3 antibody [EPR8658] (ab154810)
      All lanes : Anti-ETV3 antibody [EPR8658] (ab154810) at 1/10000 dilution

      Lane 1 : HepG2 lysate
      Lane 2 : HeLa lysate
      Lane 3 : SHSY5Y lysate
      Lane 4 : U937 lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 57 kDa

    • Flow Cytometry (Intracellular) - Anti-ETV3 antibody [EPR8658] (ab154810)
      Flow Cytometry (Intracellular) - Anti-ETV3 antibody [EPR8658] (ab154810)

      Intracellular flow cytometric analysis of permeabilized Hela cells labeling ETV3 with ab154810 at 1/10 dilution (red) or a negative rabbit IgG (control) (green).

       

    • Anti-ETV3 antibody [EPR8658] (ab154810)
      Anti-ETV3 antibody [EPR8658] (ab154810)

    References (0)

    Publishing research using ab154810? Please let us know so that we can cite the reference in this datasheet.

    ab154810 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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