Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab23465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 50 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/375000.

Target

  • Function
    Transcriptional repressor; binds to the DNA sequence 5'-CCGGAAGT-3'.
  • Tissue specificity
    Ubiquitous.
  • Involvement in disease
    Note=A chromosomal aberration involving ETV6 is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with PDGFRB. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).
    Note=Chromosomal aberrations involving ETV6 are found in a form of acute myeloid leukemia (AML). Translocation t(12;22)(p13;q11) with MN1; translocation t(4;12)(q12;p13) with CHIC2.
    Note=Chromosomal aberrations involving ETV6 are found in childhood acute lymphoblastic leukemia (ALL). Translocations t(12;21)(p12;q22) and t(12;21)(p13;q22) with RUNX1/AML1.
    Note=A chromosomal aberration involving ETV6 is found in a form of pre-B acute myeloid leukemia. Translocation t(9;12)(p24;p13) with JAK2.
    Note=A chromosomal aberration involving ETV6 is found in myelodysplastic syndrome (MDS) with basophilia. Translocation t(5;12)(q31;p13) with ACSL6.
    Note=A chromosomal aberration involving ETV6 is found in acute eosinophilic leukemia (AEL). Translocation t(5;12)(q31;p13) with ACSL6.
    Note=A chromosomal aberration involving ETV6 is found in myelodysplastic syndrome (MDS). Translocation t(1;12)(p36.1;p13) with MDS2.
    Defects in ETV6 are a cause of myeloproliferative disorder chronic with eosinophilia (MPE) [MIM:131440]. A hematologic disorder characterized by malignant eosinophils proliferation. Note=A chromosomal aberration involving ETV6 is found in many instances of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;12) with PDGFRB on chromosome 5 creating an ETV6-PDGFRB fusion protein.
    Defects in ETV6 are a cause of acute myelogenous leukemia (AML) [MIM:601626]. AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development.
    Note=A chromosomal aberration involving ETV6 is found in acute lymphoblastic leukemia. Translocation t(9;12)(p13;p13) with PAX5.
  • Sequence similarities
    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
    Contains 1 PNT (pointed) domain.
  • Post-translational
    modifications
    Phosphorylation of Ser-257 by MAPK14 (p38) inhibits ETV6 transcriptional repression.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ETS related protein Tel1 antibody
    • ETS translocation variant 6 antibody
    • Ets variant 6 antibody
    • Ets variant gene 6 (TEL oncogene) antibody
    • ETS-related protein Tel1 antibody
    • ETV 6 antibody
    • ETV6 antibody
    • ETV6_HUMAN antibody
    • Tel antibody
    • TEL oncogene antibody
    • TEL/ABL antibody
    • TEL1 antibody
    • TEL1 oncogene antibody
    • Transcription factor ETV 6 antibody
    • Transcription factor ETV6 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling ETV6 / Tel at 4µg/ml. The arrows indicate epithelial cells of fundic glands staining positively.

References

This product has been referenced in:
  • Diakos C  et al. TEL-AML1 regulation of survivin and apoptosis via miRNA-494 and miRNA-320a. Blood 116:4885-93 (2010). WB ; Human . Read more (PubMed: 20807887) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your enquiry and your interest in our products.

I have conducted a search for you at our website and found 5 antibodies against ETV6 / Tel:

ab23465,

ab54705,

ab64909,

ab74361,

ab123445

None of them have been specifically tested for ChIP application. However, generally those antibodies which are suitable for ELISA/IHC (which require the recognition of the native form of the target protein ab23465, ab54705, ab64909) may also work in ChIP but optimization is essential and highly recommended.

If you wish to test any of these antibodies, we could certainly consider offering a discount code for you for testing. The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount.


If you need any further assistance in the future, please do not hesitate to contact me.

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Question
Answer

No. This antibody recognizes the C-terminal portion of TEL.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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