Key features and details
- Rabbit polyclonal to ETV6 / Tel
- Suitable for: ELISA, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ETV6 / Tel antibody
See all ETV6 / Tel primary antibodies
DescriptionRabbit polyclonal to ETV6 / Tel
Specificityab64909 detects endogenous levels of total ETV6 / Tel protein.
Tested applicationsSuitable for: ELISA, ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide derived from internal sequence of human ETV6/ Tel.
- Purchase matching WB positive control:Recombinant Human ETV6 / Tel protein
- HeLa cells. Extracts from HeLa cells, HepG2 cells and Jurkat cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab64909 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/500 - 1/1000.|
|WB||1/500 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).|
FunctionTranscriptional repressor; binds to the DNA sequence 5'-CCGGAAGT-3'.
Involvement in diseaseNote=A chromosomal aberration involving ETV6 is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with PDGFRB. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).
Note=Chromosomal aberrations involving ETV6 are found in a form of acute myeloid leukemia (AML). Translocation t(12;22)(p13;q11) with MN1; translocation t(4;12)(q12;p13) with CHIC2.
Note=Chromosomal aberrations involving ETV6 are found in childhood acute lymphoblastic leukemia (ALL). Translocations t(12;21)(p12;q22) and t(12;21)(p13;q22) with RUNX1/AML1.
Note=A chromosomal aberration involving ETV6 is found in a form of pre-B acute myeloid leukemia. Translocation t(9;12)(p24;p13) with JAK2.
Note=A chromosomal aberration involving ETV6 is found in myelodysplastic syndrome (MDS) with basophilia. Translocation t(5;12)(q31;p13) with ACSL6.
Note=A chromosomal aberration involving ETV6 is found in acute eosinophilic leukemia (AEL). Translocation t(5;12)(q31;p13) with ACSL6.
Note=A chromosomal aberration involving ETV6 is found in myelodysplastic syndrome (MDS). Translocation t(1;12)(p36.1;p13) with MDS2.
Defects in ETV6 are a cause of myeloproliferative disorder chronic with eosinophilia (MPE) [MIM:131440]. A hematologic disorder characterized by malignant eosinophils proliferation. Note=A chromosomal aberration involving ETV6 is found in many instances of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;12) with PDGFRB on chromosome 5 creating an ETV6-PDGFRB fusion protein.
Defects in ETV6 are a cause of acute myelogenous leukemia (AML) [MIM:601626]. AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development.
Note=A chromosomal aberration involving ETV6 is found in acute lymphoblastic leukemia. Translocation t(9;12)(p13;p13) with PAX5.
Sequence similaritiesBelongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain.
modificationsPhosphorylation of Ser-257 by MAPK14 (p38) inhibits ETV6 transcriptional repression.
- Information by UniProt
- ETS related protein Tel1 antibody
- ETS translocation variant 6 antibody
- Ets variant 6 antibody
All lanes : Anti-ETV6 / Tel antibody (ab64909) at 1/500 dilution
Lane 1 : extracts from HeLa cells
Lane 2 : extracts from HepG2 cells
Lane 3 : extracts from Jurkat cells
Lane 4 : extracts from Jurkat cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 53 kDa
Observed band size: 53 kDa
ab64909, at 1/500 dilution, staining HeLa cells by immunofluorescence in the absence (left) or presence (right) of immunsing peptide.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab64909 has been referenced in 1 publication.
- Unnikrishnan A et al. A quantitative proteomics approach identifies ETV6 and IKZF1 as new regulators of an ERG-driven transcriptional network. Nucleic Acids Res 44:10644-10661 (2016). PubMed: 27604872