Anti-ETV6 / Tel antibody (ab64909)
Key features and details
- Rabbit polyclonal to ETV6 / Tel
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ETV6 / Tel antibody
See all ETV6 / Tel primary antibodies -
Description
Rabbit polyclonal to ETV6 / Tel -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide derived from internal sequence of human ETV6/ Tel.
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Positive control
- HeLa cells. Extracts from HeLa cells, HepG2 cells and Jurkat cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab64909 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/500 - 1/1000.
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WB |
1/500 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
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Notes |
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ICC/IF
1/500 - 1/1000. |
WB
1/500 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa). |
Target
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Function
Transcriptional repressor; binds to the DNA sequence 5'-CCGGAAGT-3'. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Note=A chromosomal aberration involving ETV6 is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with PDGFRB. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).
Note=Chromosomal aberrations involving ETV6 are found in a form of acute myeloid leukemia (AML). Translocation t(12;22)(p13;q11) with MN1; translocation t(4;12)(q12;p13) with CHIC2.
Note=Chromosomal aberrations involving ETV6 are found in childhood acute lymphoblastic leukemia (ALL). Translocations t(12;21)(p12;q22) and t(12;21)(p13;q22) with RUNX1/AML1.
Note=A chromosomal aberration involving ETV6 is found in a form of pre-B acute myeloid leukemia. Translocation t(9;12)(p24;p13) with JAK2.
Note=A chromosomal aberration involving ETV6 is found in myelodysplastic syndrome (MDS) with basophilia. Translocation t(5;12)(q31;p13) with ACSL6.
Note=A chromosomal aberration involving ETV6 is found in acute eosinophilic leukemia (AEL). Translocation t(5;12)(q31;p13) with ACSL6.
Note=A chromosomal aberration involving ETV6 is found in myelodysplastic syndrome (MDS). Translocation t(1;12)(p36.1;p13) with MDS2.
Defects in ETV6 are a cause of myeloproliferative disorder chronic with eosinophilia (MPE) [MIM:131440]. A hematologic disorder characterized by malignant eosinophils proliferation. Note=A chromosomal aberration involving ETV6 is found in many instances of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;12) with PDGFRB on chromosome 5 creating an ETV6-PDGFRB fusion protein.
Defects in ETV6 are a cause of acute myelogenous leukemia (AML) [MIM:601626]. AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development.
Note=A chromosomal aberration involving ETV6 is found in acute lymphoblastic leukemia. Translocation t(9;12)(p13;p13) with PAX5. -
Sequence similarities
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain. -
Post-translational
modificationsPhosphorylation of Ser-257 by MAPK14 (p38) inhibits ETV6 transcriptional repression. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2120 Human
- Entrez Gene: 14011 Mouse
- Entrez Gene: 312777 Rat
- Omim: 600618 Human
- SwissProt: P41212 Human
- SwissProt: P97360 Mouse
- Unigene: 504765 Human
- Unigene: 269995 Mouse
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Alternative names
- ETS related protein Tel1 antibody
- ETS translocation variant 6 antibody
- Ets variant 6 antibody
see all
Images
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All lanes : Anti-ETV6 / Tel antibody (ab64909) at 1/500 dilution
Lane 1 : extracts from HeLa cells
Lane 2 : extracts from HepG2 cells
Lane 3 : extracts from Jurkat cells
Lane 4 : extracts from Jurkat cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 53 kDa
Observed band size: 53 kDa -
ab64909, at 1/500 dilution, staining HeLa cells by immunofluorescence in the absence (left) or presence (right) of immunsing peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab64909 has been referenced in 1 publication.
- Unnikrishnan A et al. A quantitative proteomics approach identifies ETV6 and IKZF1 as new regulators of an ERG-driven transcriptional network. Nucleic Acids Res 44:10644-10661 (2016). PubMed: 27604872