Product nameEuropium Conjugation Kit
Europium Conjugation Kit (ab269946) allows antibodies or proteins to be quickly and easily conjugated to high quality 200 nm Europium (Eu) chelate microspheres. The particles have been specially treated to enhance the handling of the beads and permit easy covalent attachment of antibodies and other proteins.
This product is manufactured by Expedeon, an Abcam company. It was previously called 200nm Europium Conjugation Kit. Expedeon product code 1200-0120 (1 reaction midi kit) is the same as the 40 µg size of this product.
The Eu microspheres in this kit are freeze dried. The conjugation reaction is initiated simply by reconstituting the dry mixture with your antibody, which becomes attached (via lysine residues) to the specially treated surface.
It takes 30 seconds to set up the conjugation, the hands-on time for the conjugation procedure is about 3 minutes and the conjugate is ready to use within 35 minutes. You simply pipette the biomolecule into a vial containing the particles, allow the conjugation to occur and then centrifuge to buffer exchange.
The surface treatment makes the particles resistant to aggregation and the Eu fluorescence signal allows you to reach a higher sensitivity in your immunoassay.
Additionally, unlike passive methods the conjugation procedure has only a weak dependence on the isoelectric point of the antibody. Consequently, extensive trials at different pH values are not required; all antibodies can be conjugated at one of two pHs, both of which are supplied in this kit (Reaction Buffers A and B).
We recommend that your antibody is in 10 – 50 mM MES, HEPES or MOPS at pH 6 – 7 (with no other components e.g. salt or azide) prior to conjugation.
For incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Use the FAQ to learn more about the technology, or about conjugating other proteins and peptides to Europium.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 40 µg 10x Europium Quencher 1 vial 1x Reaction Buffer A 1 vial 1x Reaction Buffer B 1 vial 1x Resuspension Buffer 1 vial 200 nm Europium Mini vial (Lyophilized) 1 vial
ab269946 has not yet been referenced specifically in any publications.